Phenotypic Variation in Clinical Isolates Did Not Affect Disinfection Efficacy Using Short-Term UV-C Radiation

Overview

Journal Microorganisms

Specialty Microbiology

Date 2023 Jun 15

PMID 37317306

Authors

Birte Knobling

Gefion Franke

Laura Carlsen

Cristina Belmar Campos

Henning Buttner

Eva M Klupp

Philipp Maximilian Maurer

Johannes K Knobloch

Affiliations

Soon will be listed here.

Abstract

Pigmentation, catalase activity and biofilm formation are virulence factors that cause resistance of to environmental stress factors including disinfectants. In recent years, automatic UV-C room disinfection gained greater importance in enhanced disinfection procedures to improve disinfection success in hospitals. In this study, we evaluated the effect of naturally occurring variations in the expression of virulence factors in clinical isolates on tolerance against UV-C radiation. Quantification of staphyloxanthin expression, catalase activity and biofilm formation for nine genetically different clinical isolates as well as reference strain ATCC 6538 were performed using methanol extraction, a visual approach assay and a biofilm assay, respectively. Log reduction values (LRV) were determined after irradiation of artificially contaminated ceramic tiles with 50 and 22 mJ/cm UV-C using a commercial UV-C disinfection robot. A wide variety of virulence factor expression was observed, indicating differential regulation of global regulatory networks. However, no direct correlation with the strength of expression with UV-C tolerance was observed for either staphyloxanthin expression, catalase activity or biofilm formation. All isolates were effectively reduced with LRVs of 4.75 to 5.94. UV-C disinfection seems therefore effective against a wide spectrum of strains independent of occurring variations in the expression of the investigated virulence factors. Due to only minor differences, the results of frequently used reference strains seem to be representative also for clinical isolates in .

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