Alteration of Colonic Bacterial and Fungal Composition and Their Inter- and Intra-Kingdom Interaction in Patients with Adenomas with Low-Grade Dysplasia

Overview

Journal Microorganisms

Specialty Microbiology

Date 2023 Jun 15

PMID 37317301

Authors

Ding Heng

Min Zhang

Yuhan Yuan

Xinyun Qiu

Affiliations

Soon will be listed here.

Abstract

Colorectal cancer (CRC) develops from pre-cancerous cellular lesions in the gut epithelium and mainly originates from specific types of colonic adenomas with dysplasia. However, gut microbiota signatures among sampling sites in patients with colorectal adenomas with low-grade dysplasia (ALGD) and normal control (NC) remain uncharacterized. To characterize gut microbial and fungal profiles in ALGD and normal colorectal mucosa tissues. We used and gene sequencing and bioinformatics analysis on the microbiota of ALGD and normal colorectal mucosa from 40 subjects. Bacterial sequences in the ALGD group showed an increase in , and several genera, including , and compared to the NC group. Fungal sequences in the ALGD group showed an increase in , and , while several orders, families, and genera, including , and were decreased. The study found various interactions between intestinal bacteria and fungi. The bacterial functional analysis showed increased glycogen and vanillin degradation pathways in the ALGD group. Meanwhile, the fungal functional analysis showed a decrease in pathways related to the biosynthesis of gondoate and stearate, as well as degradation of glucose, starch, glycogen, sucrose, L-tryptophan, and pantothenate, and an increase in the octane oxidation pathway in the ALGD group. The mucosal microbiota in ALGD exhibits altered fungal and microbial composition compared to the NC mucosa, potentially contributing to the development of intestinal cancer by regulating specific metabolic pathways. Therefore, these changes in microbiota and metabolic pathways may be potential markers for diagnosing and treating colorectal adenoma and carcinoma.

References

Sobhani I . DNA Methylation Is a Main Key for Bacteria-Related Colon Carcinogenesis. Microorganisms. 2021; 9(12). PMC: 8707774. DOI: 10.3390/microorganisms9122574. View

Wheatley R, Diaz Caballero J, van der Schalk T, De Winter F, Shaw L, Kapel N . Gut to lung translocation and antibiotic mediated selection shape the dynamics of Pseudomonas aeruginosa in an ICU patient. Nat Commun. 2022; 13(1):6523. PMC: 9681761. DOI: 10.1038/s41467-022-34101-2. View

Gubatan J, Boye T, Temby M, Sojwal R, Holman D, Sinha S . Gut Microbiome in Inflammatory Bowel Disease: Role in Pathogenesis, Dietary Modulation, and Colitis-Associated Colon Cancer. Microorganisms. 2022; 10(7). PMC: 9316834. DOI: 10.3390/microorganisms10071371. View

Wu J, Xu S, Xiang C, Cao Q, Li Q, Huang J . Tongue Coating Microbiota Community and Risk Effect on Gastric Cancer. J Cancer. 2018; 9(21):4039-4048. PMC: 6218773. DOI: 10.7150/jca.25280. View

Qian L, Gao R, Hong L, Pan C, Li H, Huang J . Association analysis of dietary habits with gut microbiota of a native Chinese community. Exp Ther Med. 2018; 16(2):856-866. PMC: 6090428. DOI: 10.3892/etm.2018.6249. View

Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett W . Metagenomic biomarker discovery and explanation. Genome Biol. 2011; 12(6):R60. PMC: 3218848. DOI: 10.1186/gb-2011-12-6-r60. View

Rinninella E, Tohumcu E, Raoul P, Fiorani M, Cintoni M, Mele M . The role of diet in shaping human gut microbiota. Best Pract Res Clin Gastroenterol. 2023; 62-63:101828. DOI: 10.1016/j.bpg.2023.101828. View

Kazmierczak-Siedlecka K, Dvorak A, Folwarski M, Daca A, Przewlocka K, Makarewicz W . Fungal Gut Microbiota Dysbiosis and Its Role in Colorectal, Oral, and Pancreatic Carcinogenesis. Cancers (Basel). 2020; 12(5). PMC: 7281455. DOI: 10.3390/cancers12051326. View

Zhu Y, Shi T, Lu X, Xu Z, Qu J, Zhang Z . Fungal-induced glycolysis in macrophages promotes colon cancer by enhancing innate lymphoid cell secretion of IL-22. EMBO J. 2021; 40(11):e105320. PMC: 8167358. DOI: 10.15252/embj.2020105320. View

10.

Kim D, Jung J, Oh H, Jee S, Park S, Lee S . Comparison of sampling methods in assessing the microbiome from patients with ulcerative colitis. BMC Gastroenterol. 2021; 21(1):396. PMC: 8614001. DOI: 10.1186/s12876-021-01975-3. View

11.

Klymiuk I, Singer G, Castellani C, Trajanoski S, Obermuller B, Till H . Characterization of the Luminal and Mucosa-Associated Microbiome along the Gastrointestinal Tract: Results from Surgically Treated Preterm Infants and a Murine Model. Nutrients. 2021; 13(3). PMC: 8004827. DOI: 10.3390/nu13031030. View

12.

Chattopadhyay I, Dhar R, Pethusamy K, Seethy A, Srivastava T, Sah R . Exploring the Role of Gut Microbiome in Colon Cancer. Appl Biochem Biotechnol. 2021; 193(6):1780-1799. DOI: 10.1007/s12010-021-03498-9. View

13.

Mims T, Al Abdallah Q, Stewart J, Watts S, White C, Rousselle T . The gut mycobiome of healthy mice is shaped by the environment and correlates with metabolic outcomes in response to diet. Commun Biol. 2021; 4(1):281. PMC: 7935979. DOI: 10.1038/s42003-021-01820-z. View

14.

Yang J, Howe A, Lee J, Yoo K, Park J . An Improved Approach to Identify Bacterial Pathogens to Human in Environmental Metagenome. J Microbiol Biotechnol. 2020; 30(9):1335-1342. PMC: 9728302. DOI: 10.4014/jmb.2005.05033. View

15.

Saito K, Koido S, Odamaki T, Kajihara M, Kato K, Horiuchi S . Metagenomic analyses of the gut microbiota associated with colorectal adenoma. PLoS One. 2019; 14(2):e0212406. PMC: 6386391. DOI: 10.1371/journal.pone.0212406. View

16.

Fang C, Chen J, Hsu B, Hussain B, Rathod J, Lee K . Colorectal Cancer Stage-Specific Fecal Bacterial Community Fingerprinting of the Taiwanese Population and Underpinning of Potential Taxonomic Biomarkers. Microorganisms. 2021; 9(8). PMC: 8401100. DOI: 10.3390/microorganisms9081548. View

17.

Qiu X, Zhao X, Cui X, Mao X, Tang N, Jiao C . Characterization of fungal and bacterial dysbiosis in young adult Chinese patients with Crohn's disease. Therap Adv Gastroenterol. 2020; 13:1756284820971202. PMC: 7672770. DOI: 10.1177/1756284820971202. View

18.

Zhang Y, Yu X, Yu E, Wang N, Cai Q, Shuai Q . Changes in gut microbiota and plasma inflammatory factors across the stages of colorectal tumorigenesis: a case-control study. BMC Microbiol. 2018; 18(1):92. PMC: 6114884. DOI: 10.1186/s12866-018-1232-6. View

19.

Vallianou N, Kounatidis D, Christodoulatos G, Panagopoulos F, Karampela I, Dalamaga M . Mycobiome and Cancer: What Is the Evidence?. Cancers (Basel). 2021; 13(13). PMC: 8269322. DOI: 10.3390/cancers13133149. View

20.

Sharma N, Sarode S, Sarode G, Patil S, Pal J . Dietary Choices Modulate Colorectal Cancer Stem Cells: A Role of FXR Nuclear Receptor. Nutr Cancer. 2020; 73(7):1253-1260. DOI: 10.1080/01635581.2020.1792949. View