Identification and Characterization of a Vancomycin Intermediate-Resistant Isolated from Guangzhou, China

Overview

Journal Infect Drug Resist

Publisher Dove Medical Press

Date 2023 Jun 14

PMID 37313263

Authors

Wanyang Dong

Qi Peng

Xiaohua Tang

Tian Zhong

Shunan Lin

Ziling Zhi

Jingyi Ye

Bixia Yang

Ning Sun

Wenchang Yuan

Affiliations

Soon will be listed here.

Abstract

Background: is an opportunistic pathogen that belongs to coagulase-negative (CoNS). Increasing infection and multi-drug resistance cases caused by this strain have been reported and thus it poses a great health threat.

Methods: The third-generation sequencing technology was performed on a SH-1 isolated from a clinical sample to analyze the drug resistance genes, which included vancomycin resistance related genes. In addition, antimicrobial susceptibility tests, transmission electron microscopy and Triton X-100 stimulated autolysis were conducted to understand its biological characteristics.

Results: The study shows that this clinical isolate is a vancomycin intermediate-resistant strain. Genome comparison also revealed that WalK(N70K) and WalK(R280Q) mutations may contribute to the vancomycin resistant phenotype. Besides, SH-1 exhibit common features of thicker cell wall and decreased autolytic activity.

Conclusion: SH-1 with WalKR mutations shows typical characteristics of vancomycin resistant strains. Combining the genome features and biological properties, our findings may provide important information for the understanding of the molecular mechanism of to vancomycin intermediate-resistance.

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