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Linc-ROR Regulates and by Competitively Binding MiR-145-5p to Affect the Proliferation and Migration of Gastric Cancer Cells

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Journal Cell Transplant
Date 2023 Jun 12
PMID 37306240
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Abstract

Gastric cancer is the third leading cause of cancer-related deaths worldwide, and research on gastric cancer pathogenesis is fundamental. Long intergenic non-coding RNAs (lincRNAs) control cancer initiation and progression through several mechanisms, with the competitive endogenous RNA (ceRNA) regulatory network being the most common. In this study, hybridization revealed that long intergenic non-protein coding RNA-regulator of reprogramming (linc-ROR) was highly expressed in gastric cancer cells and was mainly cytoplasmic-positive. Cell counting kit-8 (CCK-8), plate colony formation, wound healing, and Transwell assay revealed that linc-ROR knockdown impedes the growth, proliferation, and migration of gastric cancer cells, while linc-ROR overexpression promoted gastric cancer cell growth, migration, and colony formation ability. Combined with previous studies, the molecular mechanism axis of linc-RORmiR-145-5-5p// was verified. The expression of linc-ROR knockdown significantly suppressed the protein expression of POU5F1 and SOX2. Co-transfection with linc-ROR siRNA reverses the carcinogenic effect of the miR-145-5p inhibitor on gastric cancer cell proliferation, cloning, and migration. These findings lay a foundation for developing novel targets for gastric cancer treatment.

Citing Articles

Role of miRNA‑145‑5p in cancer (Review).

Chen Z, Qin Y Oncol Rep. 2025; 53(3).

PMID: 39886965 PMC: 11800069. DOI: 10.3892/or.2025.8872.

References
1.
Hou L, Tu J, Cheng F, Yang H, Yu F, Wang M . Long noncoding RNA ROR promotes breast cancer by regulating the TGF-β pathway. Cancer Cell Int. 2018; 18:142. PMC: 6145201. DOI: 10.1186/s12935-018-0638-4. View

2.
. Japanese gastric cancer treatment guidelines 2014 (ver. 4). Gastric Cancer. 2016; 20(1):1-19. PMC: 5215069. DOI: 10.1007/s10120-016-0622-4. View

3.
Yu X, Ding H, Shi Y, Yang L, Zhou J, Yan Z . Downregulated Expression of linc-ROR in Gastric Cancer and Its Potential Diagnostic and Prognosis Value. Dis Markers. 2020; 2020:7347298. PMC: 7607276. DOI: 10.1155/2020/7347298. View

4.
Pastushenko I, Blanpain C . EMT Transition States during Tumor Progression and Metastasis. Trends Cell Biol. 2018; 29(3):212-226. DOI: 10.1016/j.tcb.2018.12.001. View

5.
Liu H, Zou Y, Zhu W, Sen-Liu , Zhang G, Ma R . lncRNA THAP7-AS1, transcriptionally activated by SP1 and post-transcriptionally stabilized by METTL3-mediated m6A modification, exerts oncogenic properties by improving CUL4B entry into the nucleus. Cell Death Differ. 2021; 29(3):627-641. PMC: 8901790. DOI: 10.1038/s41418-021-00879-9. View