Neutrophil Extracellular Trap Burden Correlates with the Stenosis of Coronary Atherosclerosis

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2023 Jun 12

PMID 37304868

Authors

Yan Wang

Mao Yang

Yuanyuan Xu

Shujun Yan

Enze Jin

Xueqi Li

Affiliations

Soon will be listed here.

Abstract

Background: Coronary atherosclerosis diseases (CADs) are associated with chronic inflammation. Neutrophil extracellular traps (NETs) are a type of novel proinflammatory cytokines whose levels are dramatically elevated in acute coronary syndrome. We conducted this study to further evaluate the association between circulating NET-associated markers and CAD in Chinese adults.

Methods: A total of 174 patients with CAD and 55 healthy controls were screened using percutaneous coronary intervention or coronary computed tomography angiography. Blood lipid levels, blood glucose levels, and blood cell counts were determined using commercial kits. Serum levels of myeloperoxidase (MPO) and neutrophil elastase (NE) were measured using ELISA. Double-stranded DNA (dsDNA) in serum was quantified using the Quant-iT PicoGreen assay. We also compared the circulating NET levels with various parameters in the study subjects.

Results: The levels of serum NET markers, dsDNA, MPO, and NE, were significantly elevated in patients with CAD, particularly in the severe group, consistent with the increase in neutrophil counts. The levels of NET markers correlated with the risk factors of AS, increasing with the number of risk factors. NET markers were identified as independent risk factors for severe coronary stenosis and also as predictors of severe CAD.

Conclusion: NETs may be related to AS and serve as indicators or predictors of stenosis in patients with severe CAD.

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References

Doring Y, Drechsler M, Wantha S, Kemmerich K, Lievens D, Vijayan S . Lack of neutrophil-derived CRAMP reduces atherosclerosis in mice. Circ Res. 2012; 110(8):1052-6. DOI: 10.1161/CIRCRESAHA.112.265868. View

Kats D, Patel M, Palta P, Meyer M, Gross A, Whitsel E . Social support and cognition in a community-based cohort: the Atherosclerosis Risk in Communities (ARIC) study. Age Ageing. 2016; 45(4):475-80. PMC: 5006245. DOI: 10.1093/ageing/afw060. View

Quan W, Hu P, Zhao X, Lianhua C, Batu B . Expression level of lncRNA PVT1 in serum of patients with coronary atherosclerosis disease and its clinical significance. Eur Rev Med Pharmacol Sci. 2020; 24(11):6333-6337. DOI: 10.26355/eurrev_202006_21531. View

Meegan J, Yang X, Coleman D, Jannaway M, Yuan S . Neutrophil-mediated vascular barrier injury: Role of neutrophil extracellular traps. Microcirculation. 2017; 24(3). PMC: 5404986. DOI: 10.1111/micc.12352. View

Grayson P, Kaplan M . At the Bench: Neutrophil extracellular traps (NETs) highlight novel aspects of innate immune system involvement in autoimmune diseases. J Leukoc Biol. 2015; 99(2):253-64. PMC: 4718195. DOI: 10.1189/jlb.5BT0615-247R. View

Glass C, Witztum J . Atherosclerosis. the road ahead. Cell. 2001; 104(4):503-16. DOI: 10.1016/s0092-8674(01)00238-0. View

Brinkmann V, Reichard U, Goosmann C, Fauler B, Uhlemann Y, Weiss D . Neutrophil extracellular traps kill bacteria. Science. 2004; 303(5663):1532-5. DOI: 10.1126/science.1092385. View

Mangold A, Alias S, Scherz T, Hofbauer T, Jakowitsch J, Panzenbock A . Coronary neutrophil extracellular trap burden and deoxyribonuclease activity in ST-elevation acute coronary syndrome are predictors of ST-segment resolution and infarct size. Circ Res. 2014; 116(7):1182-92. DOI: 10.1161/CIRCRESAHA.116.304944. View

Nauseef W, Borregaard N . Neutrophils at work. Nat Immunol. 2014; 15(7):602-11. DOI: 10.1038/ni.2921. View

10.

Farmer J, Torre-Amione G . Atherosclerosis and inflammation. Curr Atheroscler Rep. 2002; 4(2):92-8. DOI: 10.1007/s11883-002-0031-5. View

11.

Wang Y, Li M, Stadler S, Correll S, Li P, Wang D . Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation. J Cell Biol. 2009; 184(2):205-13. PMC: 2654299. DOI: 10.1083/jcb.200806072. View

12.

Sabanoglu C, Inanc I . C-reactive protein to albumin ratio predicts for severity of coronary artery disease and ischemia. Eur Rev Med Pharmacol Sci. 2022; 26(20):7623-7631. DOI: 10.26355/eurrev_202210_30038. View

13.

Li Y, Zhang C, Wang X, Liu D . Progression of atherosclerosis in ApoE-knockout mice fed on a high-fat diet. Eur Rev Med Pharmacol Sci. 2016; 20(18):3863-3867. View

14.

Warnatsch A, Ioannou M, Wang Q, Papayannopoulos V . Inflammation. Neutrophil extracellular traps license macrophages for cytokine production in atherosclerosis. Science. 2015; 349(6245):316-20. PMC: 4854322. DOI: 10.1126/science.aaa8064. View

15.

Fuchs T, Brill A, Duerschmied D, Schatzberg D, Monestier M, Myers Jr D . Extracellular DNA traps promote thrombosis. Proc Natl Acad Sci U S A. 2010; 107(36):15880-5. PMC: 2936604. DOI: 10.1073/pnas.1005743107. View

16.

Alves J, Ames P . Atherosclerosis, oxidative stress and auto-antibodies in systemic lupus erythematosus and primary antiphospholipid syndrome. Immunobiology. 2003; 207(1):23-8. DOI: 10.1078/0171-2985-00215. View

17.

Kianoush S, Bittencourt M, Lotufo P, Bensenor I, Jones S, DeFilippis A . Association Between Smoking and Serum GlycA and High-Sensitivity C-Reactive Protein Levels: The Multi-Ethnic Study of Atherosclerosis (MESA) and Brazilian Longitudinal Study of Adult Health (ELSA-Brasil). J Am Heart Assoc. 2017; 6(8). PMC: 5586473. DOI: 10.1161/JAHA.117.006545. View

18.

Qin J, Fu S, Speake C, Greenbaum C, Odegard J . NETosis-associated serum biomarkers are reduced in type 1 diabetes in association with neutrophil count. Clin Exp Immunol. 2016; 184(3):318-22. PMC: 4872375. DOI: 10.1111/cei.12783. View

19.

Jia H, Dai J, Hou J, Xing L, Ma L, Liu H . Effective anti-thrombotic therapy without stenting: intravascular optical coherence tomography-based management in plaque erosion (the EROSION study). Eur Heart J. 2016; 38(11):792-800. DOI: 10.1093/eurheartj/ehw381. View

20.

Wang H, Wang Q, Venugopal J, Wang J, Kleiman K, Guo C . Obesity-induced Endothelial Dysfunction is Prevented by Neutrophil Extracellular Trap Inhibition. Sci Rep. 2018; 8(1):4881. PMC: 5861102. DOI: 10.1038/s41598-018-23256-y. View