Comparative Performance Evaluation of Blood Film Microscopy for the Diagnosis of Bovine Trypanosomosis by Some Laboratories in North-central Nigeria

Overview

Journal Open Vet J

Specialty Veterinary Medicine

Date 2023 Jun 12

PMID 37304603

Authors

Jones Soladoye Akinbobola

Solomon Oluwole Okaiyeto

Anthony Kojo Sackey

Lushaikyaa Allam

Bisalla Mohammed

Jerome Nyhalah Dinga

Prosper C Chukwuemeka

Lucas Cunningham

Affiliations

Soon will be listed here.

Abstract

Background: Due to its affordability in disease-affected communities and suitability for field application, microscopy has historically been considered the gold standard for field diagnosis of trypanosomosis in rural settings.

Aim: This works aims to compare the performance of microscopists on bovine trypanosome microscopy by organizing the first comparative assessment on a correct reading of slides by laboratory professionals using the read slide results and a structured interviewer-administered questionnaire in North-central Nigeria.

Methods: Ten participants were addressed, as they were sent a panel of two slides (Slide 1: No Trypanosome present; Slide 2: Trypanosome present) and a questionnaire.

Results: All participants greater than 41 years old reported correctly the presence and absence of parasites on slides. Only 3/8 of microscopists from routine diagnostic laboratories reported correctly the presence of the parasite.

Conclusion: Our study confirmed errors in reading slides. Therefore, training of microscopists besides a nationwide quality assessment is recommended.

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