Berbamine, a Bioactive Alkaloid, Suppresses Equine Herpesvirus Type 1 and

Overview

Journal Front Vet Sci

Specialty Veterinary Medicine

Date 2023 Jun 12

PMID 37303732

Authors

Zeyu Li

Yuanxiu He

Lijuan Ge

Ran Quan

Junzhen Chen

Yue Hu

Ruixue Sa

Jianhua Liu

Duoliang Ran

Qiang Fu

Huijun Shi

Affiliations

Soon will be listed here.

Abstract

Equine herpesvirus type 1 (EHV-1) poses a global threat to equines. The anticancer agent berbamine (BBM), a bioactive alkaloid, has been shown to inhibit viral infection. However, whether BBM can inhibit EHV-1 infection remains unclear. This study investigated the effect of BBM treatment on EHV-1 infection. Quantitative PCR (qPCR), immunoblotting, the Reed-Muench method, and pathological examination were employed to study the ability of BBM to inhibit EHV-1 infection, viral DNA replication, viral protein production, virion secretion, and cytopathogenesis and . The studies revealed that 10 μM BBM effectively suppressed EHV-1 viral entry into cells, viral DNA replication, and virion secretion, while the studies verified the ability of BBM to suppress EHV-1-induced damage of brain and lung tissues and animal mortality. These findings strongly suggest that BBM could be a serious contender in the therapeutic control of EHV-1 infection of equines.

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PMID: 39767659 PMC: 11673035. DOI: 10.3390/biomedicines12122752.

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