Self-cloning of the Catalase Gene in Environmental Isolates Improves Their Colony-forming Abilities on Agar Media

Overview

Journal Microbes Environ

Specialty Environmental Health

Date 2023 Jun 11

PMID 37302843

Authors

Motoyuki Watanabe

Kensuke Igarashi

Souichiro Kato

Yoichi Kamagata

Wataru Kitagawa

Affiliations

Soon will be listed here.

Abstract

Hydrogen peroxide (HO) inhibits microbial growth at a specific concentration. However, we previously isolated two environmental bacterial strains that exhibited sensitivity to a lower HO concentration in agar plates. Putative catalase genes, which degrade HO, were detected in their genomes. We herein elucidated the characteristics of these putative genes and their products using a self-cloning technique. The products of the cloned genes were identified as functional catalases. The up-regulation of their expression increased the colony-forming ability of host cells under HO pressure. The present results demonstrated high sensitivity to HO even in microbes possessing functional catalase genes.

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