Bioefficacy of Nga-Mon () Fresh and Dry Leaf: Assessment of Antioxidant, Antimutagenicity, and Anti-Inflammatory Potential

Overview

Journal Plants (Basel)

Date 2023 Jun 10

PMID 37299189

Authors

Payungsak Tantipaiboonwong

Komsak Pintha

Wittaya Chaiwangyen

Maitree Suttajit

Chakkrit Khanaree

Orawan Khantamat

Affiliations

Soon will be listed here.

Abstract

Perilla leaves are known to be a rich source of polyphenols, which have been shown to exhibit various biological effects. This study aimed to compare the bioefficacies and bioactivities of fresh (PLE) and dry (PLE) Thai perilla (Nga-mon) leaf extracts. Phytochemical analysis indicated that both PLE and PLE were abundant in rosmarinic acid and bioactive phenolic compounds. PLE, which had higher levels of rosmarinic acid but lower levels of ferulic acid and luteolin than PLE, exhibited greater effectiveness in a free radical scavenging assay. Furthermore, both extracts were found to suppress intracellular ROS generation and exhibit antimutagenic activity against food-borne carcinogens in . They also attenuated lipopolysaccharide-induced inflammation in RAW 264.7 cells by inhibiting the expression of nitric oxide, iNOS, COX-2, TNF-α, IL-1β, and IL-6 through the suppression of NF-κB activation and translocation. However, PLE exhibited a higher ability to suppress cellular ROS production and higher antimutagenic and anti-inflammatory activities than PLE, which can be attributed to its combination of phytochemical components. Overall, PLE and PLE have the potential to serve as natural bioactive antioxidant, antimutagenic, and anti-inflammatory agents to achieve potential health benefits.

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References

Sarkar D, Fisher P . Molecular mechanisms of aging-associated inflammation. Cancer Lett. 2005; 236(1):13-23. DOI: 10.1016/j.canlet.2005.04.009. View

Ahmed H . Ethnomedicinal, Phytochemical and Pharmacological Investigations of (L.) Britt. Molecules. 2019; 24(1). PMC: 6337106. DOI: 10.3390/molecules24010102. View

Aderem A, Ulevitch R . Toll-like receptors in the induction of the innate immune response. Nature. 2000; 406(6797):782-7. DOI: 10.1038/35021228. View

Aktan F . iNOS-mediated nitric oxide production and its regulation. Life Sci. 2004; 75(6):639-53. DOI: 10.1016/j.lfs.2003.10.042. View

Walsh L . Mast cells and oral inflammation. Crit Rev Oral Biol Med. 2003; 14(3):188-98. DOI: 10.1177/154411130301400304. View

Yahfoufi N, Alsadi N, Jambi M, Matar C . The Immunomodulatory and Anti-Inflammatory Role of Polyphenols. Nutrients. 2018; 10(11). PMC: 6266803. DOI: 10.3390/nu10111618. View

Kim S, Vetrivel P, Kim H, Ha S, Gowda Saralamma V, Kim G . (Dowijigi) inhibits lipopolysaccharide-induced inflammation in RAW264.7 macrophages by suppressing the NF-κB signaling pathway. Exp Ther Med. 2020; 19(3):2161-2170. PMC: 7027351. DOI: 10.3892/etm.2020.8472. View

Akatsuka R, Ito M . Content and distribution of prunasin in Perilla frutescens. J Nat Med. 2022; 77(1):207-218. DOI: 10.1007/s11418-022-01654-x. View

Lee H, Han J . Anti-inflammatory Effect of Perilla frutescens (L.) Britton var. frutescens Extract in LPS-stimulated RAW 264.7 Macrophages. Prev Nutr Food Sci. 2014; 17(2):109-15. PMC: 3866756. DOI: 10.3746/pnf.2012.17.2.109. View

10.

Ooppachai C, Limtrakul Dejkriengkraikul P, Yodkeeree S . Dicentrine Potentiates TNF-α-Induced Apoptosis and Suppresses Invasion of A549 Lung Adenocarcinoma Cells via Modulation of NF-κB and AP-1 Activation. Molecules. 2019; 24(22). PMC: 6891634. DOI: 10.3390/molecules24224100. View

11.

Khantamat O, Dukaew N, Karinchai J, Chewonarin T, Pitchakarn P, Temviriyanukul P . Safety and bioactivity assessment of aqueous extract of Thai Henna ( Linn.) Leaf. J Toxicol Environ Health A. 2020; 84(7):298-312. DOI: 10.1080/15287394.2020.1866129. View

12.

Shin T, Kim S, Kim Y, Park H, Chae B, Jung H . Inhibitory effect of mast cell-mediated immediate-type allergic reactions in rats by Perilla frutescens. Immunopharmacol Immunotoxicol. 2000; 22(3):489-500. DOI: 10.3109/08923970009026007. View

13.

Nair P, McKinnon R, Miners J . Cytochrome P450 structure-function: insights from molecular dynamics simulations. Drug Metab Rev. 2016; 48(3):434-52. DOI: 10.1080/03602532.2016.1178771. View

14.

Deguchi Y, Ito M . Caffeic acid and rosmarinic acid contents in genus Perilla. J Nat Med. 2020; 74(4):834-839. DOI: 10.1007/s11418-020-01418-5. View

15.

Karin M, Lawrence T, Nizet V . Innate immunity gone awry: linking microbial infections to chronic inflammation and cancer. Cell. 2006; 124(4):823-35. DOI: 10.1016/j.cell.2006.02.016. View

16.

Yu H, Qiu J, Ma L, Hu Y, Li P, Wan J . Phytochemical and phytopharmacological review of Perilla frutescens L. (Labiatae), a traditional edible-medicinal herb in China. Food Chem Toxicol. 2016; 108(Pt B):375-391. DOI: 10.1016/j.fct.2016.11.023. View

17.

Huang B, Lin C, Chen Y, Kao S . Anti-inflammatory effects of Perilla frutescens leaf extract on lipopolysaccharide-stimulated RAW264.7 cells. Mol Med Rep. 2014; 10(2):1077-83. DOI: 10.3892/mmr.2014.2298. View

18.

Li J, Li Z, Gu L, Choi K, Kim D, Kim H . The promotion of hair regrowth by topical application of a Perilla frutescens extract through increased cell viability and antagonism of testosterone and dihydrotestosterone. J Nat Med. 2017; 72(1):96-105. DOI: 10.1007/s11418-017-1116-3. View

19.

Jiang F, Li M, Wang H, Ding B, Zhang C, Ding Z . Coelonin, an Anti-Inflammation Active Component of and Its Potential Mechanism. Int J Mol Sci. 2019; 20(18). PMC: 6770560. DOI: 10.3390/ijms20184422. View

20.

Paige J, Jaffrey S . Pharmacologic manipulation of nitric oxide signaling: targeting NOS dimerization and protein-protein interactions. Curr Top Med Chem. 2007; 7(1):97-114. DOI: 10.2174/156802607779318253. View