On the Use of Deuterated Organic Solvents Without TMS to Report H/C NMR Spectral Data of Organic Compounds: Current State of the Method, Its Pitfalls and Benefits, and Related Issues

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2023 Jun 10

PMID 37298845

Authors

Ryszard B Nazarski

Affiliations

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Abstract

The quite popular, simple but imperfect method of referencing NMR spectra to residual H and C signals of TMS-free deuterated organic solvents (referred to as Method A) is critically discussed for six commonly used NMR solvents with respect to their δ and δ values that exist in the literature. Taking into account the most reliable data, it was possible to recommend 'best' δ values for such secondary internal standards. The position of these reference points on the δ scale strongly depends on the concentration and type of analyte under study and the solvent medium used. For some solvents, chemically induced shifts (CISs) of residual H lines were considered, also taking into account the formation of 1:1 molecular complexes (for CDCl). Typical potential errors that can occur as a result of improper application of Method A are considered in detail. An overview of all found δ values adopted by users of this method revealed a discrepancy of up to 1.9 ppm in δ reported for CDCl, most likely caused by the CIS mentioned above. The drawbacks of Method A are discussed in relation to the classical use of an internal standard (Method B), two 'instrumental' schemes in which Method A is often implicitly applied, that is, the default Method C using H lock frequencies and Method D based on values, recommended by the IUPAC but only occasionally used for H/C spectra, and external referencing (Method E). Analysis of current needs and opportunities for NMR spectrometers led to the conclusion that, for the most accurate application of Method A, it is necessary to (a) use dilute solutions in a single NMR solvent and (b) to report δ data applied for the reference H/C signals to the nearest 0.001/0.01 ppm to ensure the precise characterization of new synthesized or isolated organic systems, especially those with complex or unexpected structures. However, the use of TMS in Method B is strongly recommended in all such cases.

Citing Articles

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PMID: 39942701 PMC: 11819931. DOI: 10.3390/molecules30030597.

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