Impact of Fermentation Conditions on Physicochemical Properties, Antioxidant Activity, and Sensory Properties of Apple-Tomato Pulp

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2023 Jun 10

PMID 37298839

Authors

Jing Yuan

Haiyan Zhang

Chaozhen Zeng

Juan Song

Yuwen Mu

Sanjiang Kang

Affiliations

Soon will be listed here.

Abstract

The aim of the study was to optimize the conditions [inoculum size (4, 6, and 8%), fermentation temperature (31, 34, and 37 °C), and apple: tomato ratio (2:1, 1:1, and 1:2)] on the viable cell count and sensory evaluation in apple-tomato pulp by response surface methodology (RSM), and determine the physicochemical properties, antioxidant activity, and sensory properties during fermentation. The optimal treatment parameters obtained were an inoculum size of 6.5%, a temperature of 34.5 °C, and an apple: tomato ratio of 1:1. After fermentation, the viable cell count reached 9.02 lg(CFU/mL), and the sensory evaluation score was 32.50. During the fermentation period, the pH value, total sugar, and reducing sugar decreased by 16.67%, 17.15%, and 36.05%, respectively. However, the total titratable acid (TTA), viable cell count, total phenol content (TPC), and total flavone content (TFC) increased significantly by 13.64%, 9.04%, 21.28%, and 22.22%, respectively. The antioxidant activity [2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging ability, 2,2'-azino-di(2-ethyl-benzthiazoline-sulfonic acid-6) ammonium salt (ABTS) free-radical scavenging ability, and ferric-reducing antioxidant capacity power (FRAP)] also increased by 40.91%, 22.60%, and 3.65%, respectively, during fermentation. A total of 55 volatile flavour compounds were detected using HS-SPME-GC-MS among the uninoculated samples and fermented samples before and after fermentation. The results showed that fermentation increased the types and total amount of volatile components in apple-tomato pulp, and eight new alcohols and seven new esters were formed. Alcohols, esters, and acids were the main volatile components in apple-tomato pulp, accounting for 57.39%, 10.27%, and 7.40% of the total volatile substances, respectively.

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References

Leonard W, Zhang P, Ying D, Adhikari B, Fang Z . Fermentation transforms the phenolic profiles and bioactivities of plant-based foods. Biotechnol Adv. 2021; 49:107763. DOI: 10.1016/j.biotechadv.2021.107763. View

Filannino P, Cagno R, Gobbetti M . Metabolic and functional paths of lactic acid bacteria in plant foods: get out of the labyrinth. Curr Opin Biotechnol. 2017; 49:64-72. DOI: 10.1016/j.copbio.2017.07.016. View

Fijan S . Microorganisms with claimed probiotic properties: an overview of recent literature. Int J Environ Res Public Health. 2014; 11(5):4745-67. PMC: 4053917. DOI: 10.3390/ijerph110504745. View

Yang J, Sun Y, Gao T, Wu Y, Sun H, Zhu Q . Fermentation and Storage Characteristics of "Fuji" Apple Juice Using and : Microbial Growth, Metabolism of Bioactives and Bioactivities. Front Nutr. 2022; 9:833906. PMC: 8864132. DOI: 10.3389/fnut.2022.833906. View

Badel S, Bernardi T, Michaud P . New perspectives for Lactobacilli exopolysaccharides. Biotechnol Adv. 2010; 29(1):54-66. DOI: 10.1016/j.biotechadv.2010.08.011. View

Ren Y, Li L . The influence of protease hydrolysis of lactic acid bacteria on the fermentation induced soybean protein gel: Protein molecule, peptides and amino acids. Food Res Int. 2022; 156:111284. DOI: 10.1016/j.foodres.2022.111284. View

Toi M, Hirota S, Tomotaki A, Sato N, Hozumi Y, Anan K . Probiotic Beverage with Soy Isoflavone Consumption for Breast Cancer Prevention: A Case-control Study. Curr Nutr Food Sci. 2013; 9(3):194-200. PMC: 3744907. DOI: 10.2174/15734013113099990001. View

Liao W, Shen J, Manickam S, Li S, Tao Y, Li D . Investigation of blueberry juice fermentation by mixed probiotic strains: Regression modeling, machine learning optimization and comparison with fermentation by single strain in the phenolic and volatile profiles. Food Chem. 2022; 405(Pt B):134982. DOI: 10.1016/j.foodchem.2022.134982. View

Laaksonen O, Kahala M, Marsol-Vall A, Blasco L, Jarvenpaa E, Rosenvald S . Impact of lactic acid fermentation on sensory and chemical quality of dairy analogues prepared from lupine (Lupinus angustifolius L.) seeds. Food Chem. 2021; 346:128852. DOI: 10.1016/j.foodchem.2020.128852. View

10.

Peng W, Meng D, Yue T, Wang Z, Gao Z . Effect of the apple cultivar on cloudy apple juice fermented by a mixture of Lactobacillus acidophilus, Lactobacillus plantarum, and Lactobacillus fermentum. Food Chem. 2020; 340:127922. DOI: 10.1016/j.foodchem.2020.127922. View

11.

Kober M, Bowe W . The effect of probiotics on immune regulation, acne, and photoaging. Int J Womens Dermatol. 2017; 1(2):85-89. PMC: 5418745. DOI: 10.1016/j.ijwd.2015.02.001. View

12.

Nayak B, Liu R, Tang J . Effect of processing on phenolic antioxidants of fruits, vegetables, and grains--a review. Crit Rev Food Sci Nutr. 2014; 55(7):887-919. DOI: 10.1080/10408398.2011.654142. View

13.

Wu Y, Li S, Tao Y, Li D, Han Y, Show P . Fermentation of blueberry and blackberry juices using Lactobacillus plantarum, Streptococcus thermophilus and Bifidobacterium bifidum: Growth of probiotics, metabolism of phenolics, antioxidant capacity in vitro and sensory evaluation. Food Chem. 2021; 348:129083. DOI: 10.1016/j.foodchem.2021.129083. View

14.

Silanikove N, Leitner G, Merin U . The Interrelationships between Lactose Intolerance and the Modern Dairy Industry: Global Perspectives in Evolutional and Historical Backgrounds. Nutrients. 2015; 7(9):7312-31. PMC: 4586535. DOI: 10.3390/nu7095340. View

15.

Yoon K, Woodams E, Hang Y . Probiotication of tomato juice by lactic acid bacteria. J Microbiol. 2005; 42(4):315-8. View

16.

Erel O . A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004; 37(4):277-85. DOI: 10.1016/j.clinbiochem.2003.11.015. View

17.

Bouayed J, Hoffmann L, Bohn T . Total phenolics, flavonoids, anthocyanins and antioxidant activity following simulated gastro-intestinal digestion and dialysis of apple varieties: Bioaccessibility and potential uptake. Food Chem. 2014; 128(1):14-21. DOI: 10.1016/j.foodchem.2011.02.052. View

18.

Li T, Jiang T, Liu N, Wu C, Xu H, Lei H . Biotransformation of phenolic profiles and improvement of antioxidant capacities in jujube juice by select lactic acid bacteria. Food Chem. 2020; 339:127859. DOI: 10.1016/j.foodchem.2020.127859. View

19.

Perjessy J, Hegyi F, Nagy-Gasztonyi M, Zalan Z . Effect of the lactic acid fermentation by probiotic strains on the sour cherry juice and its bioactive compounds. Food Sci Technol Int. 2021; 28(5):408-420. DOI: 10.1177/10820132211018044. View

20.

Rehman M, Jawaid P, Uchiyama H, Kondo T . Comparison of free radicals formation induced by cold atmospheric plasma, ultrasound, and ionizing radiation. Arch Biochem Biophys. 2016; 605:19-25. DOI: 10.1016/j.abb.2016.04.005. View