Effect of Continuous Chelation on the Dentinal Tubule Penetration of a Calcium Silicate-based Root Canal Sealer: a Confocal Laser Microscopy Study

Overview

Journal BMC Oral Health

Publisher Biomed Central

Specialty Dentistry

Date 2023 Jun 9

PMID 37296374

Authors

Reham Hassan

Nehal Nabil Roshdy

Affiliations

Soon will be listed here.

Abstract

Background: This study aimed to evaluate the effect of various irrigation protocols on the penetration depth of a calcium silicate-based sealer into dentinal tubules using confocal laser scanning microscopy (CLSM).

Methods: Twenty single-rooted mandibular premolars were endodontically prepared and divided into the following two groups according to the irrigation protocol used (n = 10): Group I: NaOCl + EDTA and Group II: continuous chelation (NaOCl/Dual Rinse). Obturation was performed with the warm vertical compaction technique using TotalFill HiFlow bioceramic sealer mixed with a fluorophore dye. Samples were observed using CLSM at 10× to measure the percentage of sealer penetration and its maximum depth into the dentinal tubules. Data were analysed using one-way ANOVA followed by Tukey's post-hoc test. The significance level was set at p < 0.05 within all tests.

Results: Comparing the overall results of all sections tested, no statistically significant differences existed between the groups regarding the percentage of sealer penetration (p = 0.612) and maximum depth of penetration (p > 0.05).

Conclusions: With both types of irrigation used, dentinal tubule penetration was higher in the coronal section than in the apical section. Continuous chelation using NaOCl/Dual Rinse HEDP performed better in the coronal segments, while irrigation using NaOCl + EDTA promoted a higher percentage of sealer penetration in the apical segment.

Citing Articles

Effect of various final irrigation solutions at different temperatures on dentin tubule penetration of bioceramic-based root canal sealer.

Arili Ozturk E, Canakci B, Turan Gokduman C BMC Oral Health. 2025; 25(1):352.

PMID: 40055658 PMC: 11887344. DOI: 10.1186/s12903-025-05727-7.

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