Alpha 2.0
Login Register
» Articles » PMID: 3729376

Enzymatic Characterization of Oral and Non-oral Black-pigmented Bacteroides Species

Overview
Journal Antonie Van Leeuwenhoek
Publisher Springer
Specialty Microbiology
Date 1986 Jan 1
PMID 3729376
Citations 9
Authors
A J van Winkelhoff
T J van Steenbergen
N Kippuw
J de Graaff
Affiliations
Soon will be listed here.
Abstract

The purpose of this study was to investigate the production of various enzymes by oral and non-oral black-pigmented Bacteroides species using chromogenic substrates. The 19 substrates present in the API ZYM system did not differentiate between B. melaninogenicus, B. denticola, B. loescheii and B. levii. The asaccharolytic black-pigmented Bacteroides species showed each species specific enzyme activity, however, differences were based on one enzyme only as far as B. asaccharolyticus and B. endodontalis are concerned. An extended number of 40 chromogenic substrates were tested in order to find more species specific enzyme. With a set of 20 substrates it appeared to be possible to discriminate between all species tested. The possibility to use enzymes for the identification of black-pigmented Bacteroides is discussed.

Citing Articles

Rapid characterization of periodontal bacterial isolates by using fluorogenic substrate tests.

Maiden M, Tanner A, Macuch P J Clin Microbiol. 1996; 34(2):376-84.

PMID: 8789019 PMC: 228801. DOI: 10.1128/jcm.34.2.376-384.1996.

Effects of N-acetylglucosamine on carbohydrate fermentation by Streptococcus mutans NCTC 10449 and Streptococcus sobrinus SL-1.

Homer K, Patel R, Beighton D Infect Immun. 1993; 61(1):295-302.

PMID: 8418050 PMC: 302718. DOI: 10.1128/iai.61.1.295-302.1993.

Isolation and characterization of a protease from Bacteroides gingivalis.

Fujimura S, Nakamura T Infect Immun. 1987; 55(3):716-20.

PMID: 3546137 PMC: 260399. DOI: 10.1128/iai.55.3.716-720.1987.

Enzymatic and antigenic characterization of immunoglobulin A1 proteases from Bacteroides and Capnocytophaga spp.

Frandsen E, Reinholdt J, Kilian M Infect Immun. 1987; 55(3):631-8.

PMID: 3546133 PMC: 260386. DOI: 10.1128/iai.55.3.631-638.1987.

Response of a Streptococcus sanguis strain to arginine-containing peptides.

Rogers A, Zilm P, Gully N, Pfennig A Infect Immun. 1988; 56(3):687-92.

PMID: 3343053 PMC: 259346. DOI: 10.1128/iai.56.3.687-692.1988.

References
1.
Kilian M . Rapid identification of Actinomycetaceae and related bacteria. J Clin Microbiol. 1978; 8(2):127-33. PMC: 275167. DOI: 10.1128/jcm.8.2.127-133.1978. View
2.
Marler L, Allen S, Siders J . Rapid enzymatic characterization of clinically encountered anaerobic bacteria with the API ZYM system. Eur J Clin Microbiol. 1984; 3(4):294-300. DOI: 10.1007/BF01977476. View
3.
Laughon B, Syed S, Loesche W . API ZYM system for identification of Bacteroides spp., Capnocytophaga spp., and spirochetes of oral origin. J Clin Microbiol. 1982; 15(1):97-102. PMC: 272032. DOI: 10.1128/jcm.15.1.97-102.1982. View
4.
van Steenbergen T, Vlaanderen C, de Graaff J . Deoxyribonucleic acid homologies among strains of Bacteroides melaninogenicus and related species. J Appl Bacteriol. 1982; 53(2):269-76. DOI: 10.1111/j.1365-2672.1982.tb04685.x. View
5.
Slots J . Enzymatic characterization of some oral and nonoral gram-negative bacteria with the API ZYM system. J Clin Microbiol. 1981; 14(3):288-94. PMC: 271956. DOI: 10.1128/jcm.14.3.288-294.1981. View