Effects of Undifferentiated Callus Extracts from L, L and L in Normal and Malignant Human Skin Cells

Overview

Journal Heliyon

Specialty Social Sciences

Date 2023 Jun 9

PMID 37292297

Authors

Anette Gjorloff Wingren

Riyam Ziyad Faik

Anna Holefors

Edina Filecovic

Anna Gustafsson

Affiliations

Soon will be listed here.

Abstract

Background And Objectives: The occurrence of non-melanoma and melanoma skin cancers is currently increasing rapidly with one in every three cancers diagnosed as a skin cancer. A useful strategy to control the progression of skin cancer could be the use of plant flavonoids that suppress pro-inflammatory cytokines involved in tumor initiation and progression. In this study, the anti-inflammatory and antioxidant activity of undifferentiated callus extracts from L, L and L was investigated both in normal and malignant skin cells.

Methods: Antioxidant activity of the extracts was analyzed by using the Trolox Equivalent Antioxidant Capacity (TEAC) assay. High-Performance Thin-Layer Chromatography (HPTLC) was performed to demonstrate the phytochemical profile, and the total flavonoid content was analyzed with an aluminum chloride colorimetric method. The anti-inflammatory effect was investigated by cell treatments using the plant extracts. Thereafter, the possible suppression of induced IL-6 response was measured from the cultured skin cancer cell lines A2058 and A431, and normal primary keratinocytes with Enzyme-Linked Immunosorbent Assay (ELISA).

Results: The HPTLC analysis assessed that the extracts contained a complex phytochemical profile that was rich in phenolic and flavonoid compounds. Dose response assays showed that concentrations between 15 and 125 μg/mL of all three plant extracts could be used to investigate an effect on the IL-6 production. The extract had the most pronounced anti-inflammatory effect, which significantly inhibited induced IL-6 production in both normal keratinocytes and skin cells derived from epidermal carcinoma. The extract from also had the highest flavonoid content and showed the highest antioxidant activity of the three extracts tested.

Conclusion: All in all, we have confirmed that undifferentiated callus extracts of possess properties such as antioxidant and anti-inflammatory activities in both normal and malignant keratinocytes, and thus could be a promising agent controlling the pro-inflammatory IL-6 production.

Citing Articles

A review of the botany, phytochemistry, pharmacology, synthetic biology and comprehensive utilization of .

Zhang X, Liu M, Wang Z, Wang P, Kong L, Wu J Front Pharmacol. 2024; 15:1417655.

PMID: 39055491 PMC: 11269164. DOI: 10.3389/fphar.2024.1417655.

Potential Anti-Tumorigenic Properties of Diverse Medicinal Plants against the Majority of Common Types of Cancer.

Albahri G, Badran A, Abdel Baki Z, Alame M, Hijazi A, Daou A Pharmaceuticals (Basel). 2024; 17(5).

PMID: 38794144 PMC: 11124340. DOI: 10.3390/ph17050574.

References

Jones P, Baylin S . The fundamental role of epigenetic events in cancer. Nat Rev Genet. 2002; 3(6):415-28. DOI: 10.1038/nrg816. View

Rose-John S, Scheller J, Elson G, Jones S . Interleukin-6 biology is coordinated by membrane-bound and soluble receptors: role in inflammation and cancer. J Leukoc Biol. 2006; 80(2):227-36. DOI: 10.1189/jlb.1105674. View

Kao W, Lin C, Lee L, Lee P, Hung C, Lin Y . Investigation of MMP-2 and -9 in a highly invasive A431 tumor cell sub-line selected from a Boyden chamber assay. Anticancer Res. 2008; 28(4B):2109-20. View

Ponte L, Pavan I, Mancini M, da Silva L, Morelli A, Severino M . The Hallmarks of Flavonoids in Cancer. Molecules. 2021; 26(7). PMC: 8038160. DOI: 10.3390/molecules26072029. View

Rigby C, Roy S, Deep G, Guillermo-Lagae R, Jain A, Dhar D . Role of p53 in silibinin-mediated inhibition of ultraviolet B radiation-induced DNA damage, inflammation and skin carcinogenesis. Carcinogenesis. 2016; 38(1):40-50. PMC: 5219048. DOI: 10.1093/carcin/bgw106. View

Giustizieri M, Mascia F, Frezzolini A, De Pita O, Chinni L, Giannetti A . Keratinocytes from patients with atopic dermatitis and psoriasis show a distinct chemokine production profile in response to T cell-derived cytokines. J Allergy Clin Immunol. 2001; 107(5):871-7. DOI: 10.1067/mai.2001.114707. View

Luo Y, Zheng S . Hall of Fame among Pro-inflammatory Cytokines: Interleukin-6 Gene and Its Transcriptional Regulation Mechanisms. Front Immunol. 2017; 7:604. PMC: 5165036. DOI: 10.3389/fimmu.2016.00604. View

Gospodaryov D, Yurkevych I, Jafari M, Lushchak V, Lushchak O . Lifespan extension and delay of age-related functional decline caused by Rhodiola rosea depends on dietary macronutrient balance. Longev Healthspan. 2014; 2(1):5. PMC: 3922952. DOI: 10.1186/2046-2395-2-5. View

Frankova J, Juranova J, Biedermann D, Ulrichova J . Influence of silymarin components on keratinocytes and 3D reconstructed epidermis. Toxicol In Vitro. 2021; 74:105162. DOI: 10.1016/j.tiv.2021.105162. View

10.

Kartini , Piyaviriyakul S, Thongpraditchote S, Siripong P, Vallisuta O . Effects of Extracts and Its Chemical Compounds on Proliferation of Cancer Cells and Cytokines Production of Lipopolysaccharide-activated THP-1 Macrophages. Pharmacogn Mag. 2017; 13(51):393-399. PMC: 5551355. DOI: 10.4103/pm.pm_406_16. View

11.

Park K, Chang I . Anti-inflammatory activity of aucubin by inhibition of tumor necrosis factor-alpha production in RAW 264.7 cells. Planta Med. 2004; 70(8):778-9. DOI: 10.1055/s-2004-827211. View

12.

Prasad R, Paudel S, Raina K, Agarwal R . Silibinin and non-melanoma skin cancers. J Tradit Complement Med. 2020; 10(3):236-244. PMC: 7340873. DOI: 10.1016/j.jtcme.2020.02.003. View

13.

Hung J, Yang C, Tsai Y, Huang H, Huang M . Antiproliferative activity of aucubin is through cell cycle arrest and apoptosis in human non-small cell lung cancer A549 cells. Clin Exp Pharmacol Physiol. 2008; 35(9):995-1001. DOI: 10.1111/j.1440-1681.2008.04935.x. View

14.

Apalla Z, Lallas A, Sotiriou E, Lazaridou E, Ioannides D . Epidemiological trends in skin cancer. Dermatol Pract Concept. 2017; 7(2):1-6. PMC: 5424654. DOI: 10.5826/dpc.0702a01. View

15.

Batra P, Sharma A . Anti-cancer potential of flavonoids: recent trends and future perspectives. 3 Biotech. 2017; 3(6):439-459. PMC: 3824783. DOI: 10.1007/s13205-013-0117-5. View

16.

Ciazynska M, Olejniczak-Staruch I, Sobolewska-Sztychny D, Narbutt J, Skibinska M, Lesiak A . Ultraviolet Radiation and Chronic Inflammation-Molecules and Mechanisms Involved in Skin Carcinogenesis: A Narrative Review. Life (Basel). 2021; 11(4). PMC: 8068112. DOI: 10.3390/life11040326. View

17.

Chahar M, Sharma N, Dobhal M, Joshi Y . Flavonoids: A versatile source of anticancer drugs. Pharmacogn Rev. 2011; 5(9):1-12. PMC: 3210013. DOI: 10.4103/0973-7847.79093. View

18.

Lin C, Tsai P, Kandaswami C, Chang G, Cheng C, Huang C . Role of tissue transglutaminase 2 in the acquisition of a mesenchymal-like phenotype in highly invasive A431 tumor cells. Mol Cancer. 2011; 10:87. PMC: 3150327. DOI: 10.1186/1476-4598-10-87. View

19.

Panossian A, Wikman G, Sarris J . Rosenroot (Rhodiola rosea): traditional use, chemical composition, pharmacology and clinical efficacy. Phytomedicine. 2010; 17(7):481-93. DOI: 10.1016/j.phymed.2010.02.002. View

20.

Dobrovolskaia M, Kozlov S . Inflammation and cancer: when NF-kappaB amalgamates the perilous partnership. Curr Cancer Drug Targets. 2005; 5(5):325-44. DOI: 10.2174/1568009054629645. View