Specific Expression of a Gene Encoding a Novel Calcium-binding Protein, CAF-1, During Transition of Dictyostelium Cells from Growth to Differentiation

Overview

Journal Dev Growth Differ

Specialties Biology
Reproductive Medicine

Date 2023 Jun 7

PMID 37281249

Authors

Fumiyoshi Abe

Yasuo Maeda

Affiliations

Soon will be listed here.

Abstract

The gene expressions involved in the transition from cell proliferation to differentiation were analyzed, using synchronized Dictyostelium discoideum Ax-2 cells and the differential plaque hybridization method. As one of the genes (cDNA) specifically expressed when Ax-2 cells were starved just before the putative shift (PS)-point (putative shift point; a switchover point from growth to differentiation in the cell cycle), calfumirin-1 (CAF-1) was cloned, which encoded a novel calcium-binding protein with E-F hand. Although CAF-1 mRNA was slightly expressed in vegetatively growing cells, the expression was markedly increased in response to starvation of cells just before the PS-point. Northern analysis using non-synchronized Ax-2 cells showed that the CAF-1 mRNA is predominantly expressed within a few hours of starvation. Such a starvation-induced early expression of the CAF-1 mRNA raised a possibility that CAF-1 might be one of Ca -binding proteins involved in the phase-shift of cells from growth to differentiation.

Citing Articles

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PMID: 16857047 PMC: 1634994. DOI: 10.1186/1471-2164-7-183.

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