Co-interaction of Nitrofuran Antibiotics and the Saponin-rich Extract on Gram-negative Bacteria and Colon Epithelial Cells

Overview

Journal World J Microbiol Biotechnol

Publisher Springer

Specialties Biotechnology
Microbiology

Date 2023 Jun 5

PMID 37273071

Authors

Adam Grzywaczyk

Wojciech Smulek

Anna Olejnik

Urszula Guzik

Agnieszka Nowak

Ewa Kaczorek

Affiliations

Soon will be listed here.

Abstract

Large-scale use of nitrofurans is associated with a number of risks related to a growing resistance to these compounds and the toxic effects following from their increasing presence in wastewater and the environment. The aim of the study was to investigate an impact of natural surfactant, saponins from Sapindus mukorossi, on antimicrobial properties of nitrofuran antibiotics. Measurements of bacterial metabolic activity indicated a synergistic bactericidal effect in samples with nitrofurantoin or furazolidone, to which saponins were added. Their addition led to more than 50% greater reduction in viable cells than in the samples without saponins. On the other hand, no toxic effect against human colon epithelial cell was observed. It was found that exposure to antibiotics and surfactants caused the cell membranes to be dominated by branched fatty acids. Moreover, the presence of saponins reduced the hydrophobicity of the cell surface making them almost completely hydrophilic. The results have confirmed a high affinity of saponins to the cells of Pseudomonas strains. Their beneficial synergistic effect on the action of antibiotics from the nitrofuran group was also demonstrated. This result opens promising prospects for the use of saponins from S. mukorossi as an adjuvant to reduce the emission of antibiotics into the environment.

References

Ambalam P, Kondepudi K, Nilsson I, Wadstrom T, Ljungh A . Bile stimulates cell surface hydrophobicity, Congo red binding and biofilm formation of Lactobacillus strains. FEMS Microbiol Lett. 2012; 333(1):10-9. DOI: 10.1111/j.1574-6968.2012.02590.x. View

Asokan G, Ramadhan T, Ahmed E, Sanad H . WHO Global Priority Pathogens List: A Bibliometric Analysis of Medline-PubMed for Knowledge Mobilization to Infection Prevention and Control Practices in Bahrain. Oman Med J. 2019; 34(3):184-193. PMC: 6505350. DOI: 10.5001/omj.2019.37. View

Gorny D, Guzik U, Hupert-Kocurek K, Wojcieszynska D . Naproxen ecotoxicity and biodegradation by Bacillus thuringiensis B1(2015b) strain. Ecotoxicol Environ Saf. 2018; 167:505-512. DOI: 10.1016/j.ecoenv.2018.10.067. View

Jurek I, Goral I, Gesinski K, Wojciechowski K . Effect of saponins from quinoa on a skin-mimetic lipid monolayer containing cholesterol. Steroids. 2018; 147:52-57. DOI: 10.1016/j.steroids.2018.11.008. View

Knudsen D, Jutfelt F, Sundh H, Sundell K, Koppe W, Frokiaer H . Dietary soya saponins increase gut permeability and play a key role in the onset of soyabean-induced enteritis in Atlantic salmon ( Salmo salar L.). Br J Nutr. 2008; 100(1):120-9. DOI: 10.1017/S0007114507886338. View

Liao Y, Li Z, Zhou Q, Sheng M, Qu Q, Shi Y . Saponin surfactants used in drug delivery systems: A new application for natural medicine components. Int J Pharm. 2021; 603:120709. DOI: 10.1016/j.ijpharm.2021.120709. View

Lorent J, Lins L, Domenech O, Quetin-Leclercq J, Brasseur R, Mingeot-Leclercq M . Domain formation and permeabilization induced by the saponin α-hederin and its aglycone hederagenin in a cholesterol-containing bilayer. Langmuir. 2014; 30(16):4556-69. DOI: 10.1021/la4049902. View

Muniyan A, Ravi K, Mohan U, Panchamoorthy R . Characterization and in vitro antibacterial activity of saponin-conjugated silver nanoparticles against bacteria that cause burn wound infection. World J Microbiol Biotechnol. 2017; 33(7):147. DOI: 10.1007/s11274-017-2309-3. View

Nadanaciva S, Dillman K, Gebhard D, Shrikhande A, Will Y . High-content screening for compounds that affect mtDNA-encoded protein levels in eukaryotic cells. J Biomol Screen. 2010; 15(8):937-48. DOI: 10.1177/1087057110373547. View

10.

Nowak A, Mrozik A . Facilitation of Co-Metabolic Transformation and Degradation of Monochlorophenols by sp. CF600 and Changes in Its Fatty Acid Composition. Water Air Soil Pollut. 2016; 227:83. PMC: 4752961. DOI: 10.1007/s11270-016-2775-5. View

11.

Pacholak A, Burlaga N, Guzik U, Kaczorek E . Investigation of the bacterial cell envelope nanomechanical properties after long-term exposure to nitrofurans. J Hazard Mater. 2020; 407:124352. DOI: 10.1016/j.jhazmat.2020.124352. View

12.

Pacholak A, Juzwa W, Zgola-Grzeskowiak A, Kaczorek E . Multi-faceted analysis of bacterial transformation of nitrofurantoin. Sci Total Environ. 2023; 874:162422. DOI: 10.1016/j.scitotenv.2023.162422. View

13.

Polianciuc S, Gurzau A, Kiss B, Stefan M, Loghin F . Antibiotics in the environment: causes and consequences. Med Pharm Rep. 2020; 93(3):231-240. PMC: 7418837. DOI: 10.15386/mpr-1742. View

14.

Rojewska M, Smulek W, Prochaska K, Kaczorek E . Combined Effect of Nitrofurantoin and Plant Surfactant on Bacteria Phospholipid Membrane. Molecules. 2020; 25(11). PMC: 7321062. DOI: 10.3390/molecules25112527. View

15.

Rojewska M, Smulek W, Grzywaczyk A, Kaczorek E, Prochaska K . Study of Interactions between Saponin Biosurfactant and Model Biological Membranes: Phospholipid Monolayers and Liposomes. Molecules. 2023; 28(4). PMC: 9961525. DOI: 10.3390/molecules28041965. View

16.

Ropponen H, Richter R, Hirsch A, Lehr C . Mastering the Gram-negative bacterial barrier - Chemical approaches to increase bacterial bioavailability of antibiotics. Adv Drug Deliv Rev. 2021; 172:339-360. DOI: 10.1016/j.addr.2021.02.014. View

17.

Smulek W, Kaczorek E . Factors Influencing the Bioavailability of Organic Molecules to Bacterial Cells-A Mini-Review. Molecules. 2022; 27(19). PMC: 9570905. DOI: 10.3390/molecules27196579. View

18.

Smulek W, Zdarta A, Luczak M, Krawczyk P, Jesionowski T, Kaczorek E . Sapindus saponins' impact on hydrocarbon biodegradation by bacteria strains after short- and long-term contact with pollutant. Colloids Surf B Biointerfaces. 2016; 142:207-213. DOI: 10.1016/j.colsurfb.2016.02.049. View

19.

Smulek W, Zdarta A, Grzywaczyk A, Guzik U, Siwinska-Ciesielczyk K, Ciesielczyk F . Evaluation of the physico-chemical properties of hydrocarbons-exposed bacterial biomass. Colloids Surf B Biointerfaces. 2020; 196:111310. DOI: 10.1016/j.colsurfb.2020.111310. View

20.

Sreij R, Prevost S, Dargel C, Dattani R, Hertle Y, Wrede O . Interaction of the Saponin Aescin with Ibuprofen in DMPC Model Membranes. Mol Pharm. 2018; 15(10):4446-4461. DOI: 10.1021/acs.molpharmaceut.8b00421. View