A Lysosome-Targeted Tetrazine for Organelle-Specific Click-to-Release Chemistry in Antigen Presenting Cells

Overview

Journal J Am Chem Soc

Publisher American Chemical Society

Specialty Chemistry

Date 2023 Jun 3

PMID 37269296

Authors

Nina A M Ligthart

Mark A R de Geus

Merel A T van de Plassche

Diana Torres Garcia

Marjolein M E Isendoorn

Luuk Reinalda

Danielle Ofman

Tyrza van Leeuwen

Sander I van Kasteren

Affiliations

Soon will be listed here.

Abstract

Bioorthogonal deprotections are readily used to control biological function in a cell-specific manner. To further improve the spatial resolution of these reactions, we here present a lysosome-targeted tetrazine for an organelle-specific deprotection reaction. We show that -cyclooctene deprotection with this reagent can be used to control the biological activity of ligands for invariant natural killer T cells in the lysosome to shed light on the processing pathway in antigen presenting cells. We then use the lysosome-targeted tetrazine to show that long peptide antigens used for CD8 T cell activation do not pass through this organelle, suggesting a role for the earlier endosomal compartments for their processing.

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