Statistical Optimization of P(3HB-co-3HHx) Copolymers Production by Cupriavidus Necator PHB4/pBBR_CnPro-phaC and Its Properties Characterization

Overview

Journal Sci Rep

Specialty Science

Date 2023 Jun 2

PMID 37268758

Authors

Chanaporn Trakunjae

Antika Boondaeng

Waraporn Apiwatanapiwat

Phornphimon Janchai

Soon Zher Neoh

Kumar Sudesh

Pilanee Vaithanomsat

Affiliations

Soon will be listed here.

Abstract

Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] is a bacterial copolymer in the polyhydroxyalkanoates (PHAs) family, a next-generation bioplastic. Our research team recently engineered a newly P(3HB-co-3HHx)-producing bacterial strain, Cupriavidus necator PHB4/pBBR_CnPro-phaC. This strain can produce P(3HB-co-2 mol% 3HHx) using crude palm kernel oil (CPKO) as a sole carbon substrate. However, the improvement of P(3HB-co-3HHx) copolymer production by this strain has not been studied so far. Thus, this study aims to enhance the production of P(3HB-co-3HHx) copolymers containing higher 3HHx monomer compositions using response surface methodology (RSM). Three significant factors for P(3HB-co-3HHx) copolymers production, i.e., CPKO concentration, sodium hexanoate concentration, and cultivation time, were studied in the flask scale. As a result, a maximum of 3.6 ± 0.4 g/L of P(3HB-co-3HHx) with 4 mol% 3HHx compositions was obtained using the RSM optimized condition. Likewise, the higher 3HHx monomer composition (5 mol%) was obtained when scaling up the fermentation in a 10L-stirrer bioreactor. Furthermore, the produced polymer's properties were similar to marketable P(3HB-co-3HHx), making this polymer suitable for a wide range of applications.

Citing Articles

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PMID: 38658595 PMC: 11043324. DOI: 10.1038/s41598-024-59439-z.

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