A Longitudinal Study of Blastocystis in Dairy Calves from Birth Through 24 months Demonstrates Dynamic Shifts in Infection Rates and Subtype Prevalence and Diversity by Age

Overview

Journal Parasit Vectors

Publisher Biomed Central

Specialties Parasitology
Tropical Medicine

Date 2023 Jun 1

PMID 37264466

Authors

Monica Santin

Aleksey Molokin

Jenny G Maloney

Affiliations

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Abstract

Blastocystis is a common microeukaryotic intestinal parasite in humans and other animal hosts globally. However, no large-scale longitudinal study has ever been conducted for Blastocystis. To understand patterns of infection prevalence and subtype diversity and their relationship with host age, we have conducted the most comprehensive longitudinal study of Blastocystis infection ever performed. Dairy calves from a herd located in Maryland, USA, were followed from birth through 24 months of age, and 990 individual fecal samples from 30 calves were collected over the study period, representing three age groups (pre-weaned, post-weaned, and heifer). All samples were screened for Blastocystis via PCR, and subtype determination was performed using next-generation amplicon sequencing. Associations between age group and infection status were assessed using logistic regression analyses. Blastocystis infection prevalence increased with time, significant associations were observed between age groups and infection risk, and a cumulative prevalence of 100% was observed among the study population during the 24-month period. Thirteen previously reported subtypes (ST1-6, ST10, ST14, ST21, ST23-26) and one potentially novel subtype were observed. Diversity within ST10 supports the need for division of the subtype into new subtype designations. Associations between subtype and age group were explored, and relationships between subtypes and infection chronicity are described. While subtype diversity increased with age in the study population, distinct patterns of individual subtype prevalence and chronicity were observed, supporting the importance of subtype discrimination in studies of host infection and disease. The data from this study represent a significant advance in our understanding of Blastocystis infection dynamics within a single host population over time and can be used to inform future studies of Blastocystis epidemiology in both humans and other animal hosts.

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References

Osorio-Pulgarin M, Higuera A, Beltran-Alzate J, Sanchez-Jimenez M, Ramirez J . Epidemiological and Molecular Characterization of Infection in Children Attending Daycare Centers in Medellín, Colombia. Biology (Basel). 2021; 10(7). PMC: 8301444. DOI: 10.3390/biology10070669. View

van Hattem J, Arcilla M, Schultsz C, Bootsma M, Verhaar N, Rebers S . Carriage of Blastocystis spp. in travellers - A prospective longitudinal study. Travel Med Infect Dis. 2018; 27:87-91. DOI: 10.1016/j.tmaid.2018.06.005. View

Scanlan P, Knight R, Song S, Ackermann G, Cotter P . Prevalence and genetic diversity of Blastocystis in family units living in the United States. Infect Genet Evol. 2016; 45:95-97. DOI: 10.1016/j.meegid.2016.08.018. View

Ajjampur S, Tan K . Pathogenic mechanisms in Blastocystis spp. - Interpreting results from in vitro and in vivo studies. Parasitol Int. 2016; 65(6 Pt B):772-779. DOI: 10.1016/j.parint.2016.05.007. View

Frealle E, El Safadi D, Cian A, Aubry E, Certad G, Osman M . Acute blastocystis-associated appendicular peritonitis in a child, Casablanca, Morocco. Emerg Infect Dis. 2014; 21(1):91-4. PMC: 4285265. DOI: 10.3201/eid2101.140544. View

Abarca N, Santin M, Ortega S, Maloney J, George N, Molokin A . Molecular Detection and Characterization of sp. and in Cattle in Northern Spain. Vet Sci. 2021; 8(9). PMC: 8473172. DOI: 10.3390/vetsci8090191. View

Tan K . New insights on classification, identification, and clinical relevance of Blastocystis spp. Clin Microbiol Rev. 2008; 21(4):639-65. PMC: 2570156. DOI: 10.1128/CMR.00022-08. View

Maloney J, Molokin A, Segui R, Maravilla P, Martinez-Hernandez F, Villalobos G . Identification and Molecular Characterization of Four New Subtypes Designated ST35-ST38. Microorganisms. 2023; 11(1). PMC: 9860945. DOI: 10.3390/microorganisms11010046. View

Nigro L, Larocca L, Massarelli L, Patamia I, Minniti S, Palermo F . A placebo-controlled treatment trial of Blastocystis hominis infection with metronidazole. J Travel Med. 2003; 10(2):128-30. DOI: 10.2310/7060.2003.31714. View

10.

Rojas-Velazquez L, Maloney J, Molokin A, Moran P, Serrano-Vazquez A, Gonzalez E . Use of next-generation amplicon sequencing to study Blastocystis genetic diversity in a rural human population from Mexico. Parasit Vectors. 2019; 12(1):566. PMC: 6882168. DOI: 10.1186/s13071-019-3814-z. View

11.

Nash A, Auchtung T, Wong M, Smith D, Gesell J, Ross M . The gut mycobiome of the Human Microbiome Project healthy cohort. Microbiome. 2017; 5(1):153. PMC: 5702186. DOI: 10.1186/s40168-017-0373-4. View

12.

Jinatham V, Maxamhud S, Popluechai S, Tsaousis A, Gentekaki E . One Health Approach in a Rural Community of Northern Thailand: Prevalence, Subtypes and Novel Transmission Routes. Front Microbiol. 2021; 12:746340. PMC: 8696170. DOI: 10.3389/fmicb.2021.746340. View

13.

Ramirez J, Sanchez A, Hernandez C, Florez C, Bernal M, Giraldo J . Geographic distribution of human Blastocystis subtypes in South America. Infect Genet Evol. 2016; 41:32-35. DOI: 10.1016/j.meegid.2016.03.017. View

14.

Maloney J, Lombard J, Urie N, Shivley C, Santin M . Zoonotic and genetically diverse subtypes of Blastocystis in US pre-weaned dairy heifer calves. Parasitol Res. 2018; 118(2):575-582. DOI: 10.1007/s00436-018-6149-3. View

15.

Herwaldt B, de Arroyave K, Wahlquist S, De Merida A, Lopez A, Juranek D . Multiyear prospective study of intestinal parasitism in a cohort of Peace Corps volunteers in Guatemala. J Clin Microbiol. 2001; 39(1):34-42. PMC: 87675. DOI: 10.1128/JCM.39.1.34-42.2001. View

16.

Ihekweazu F, Versalovic J . Development of the Pediatric Gut Microbiome: Impact on Health and Disease. Am J Med Sci. 2018; 356(5):413-423. PMC: 6268214. DOI: 10.1016/j.amjms.2018.08.005. View

17.

Moreno Y, Moreno-Mesonero L, Amoros I, Perez R, Morillo J, Alonso J . Multiple identification of most important waterborne protozoa in surface water used for irrigation purposes by 18S rRNA amplicon-based metagenomics. Int J Hyg Environ Health. 2017; 221(1):102-111. DOI: 10.1016/j.ijheh.2017.10.008. View

18.

Aykur M, Camyar A, Turk B, Sin A, Dagci H . Evaluation of association with subtypes and alleles of Blastocystis with chronic spontaneous urticaria. Acta Trop. 2022; 231:106455. DOI: 10.1016/j.actatropica.2022.106455. View

19.

Higuera A, Herrera G, Jimenez P, Garcia-Corredor D, Pulido-Medellin M, Bulla-Castaneda D . Identification of Multiple Subtypes in Domestic Animals From Colombia Using Amplicon-Based Next Generation Sequencing. Front Vet Sci. 2021; 8:732129. PMC: 8421793. DOI: 10.3389/fvets.2021.732129. View

20.

Maloney J, da Cunha M, Molokin A, Cury M, Santin M . Next-generation sequencing reveals wide genetic diversity of Blastocystis subtypes in chickens including potentially zoonotic subtypes. Parasitol Res. 2021; 120(6):2219-2231. DOI: 10.1007/s00436-021-07170-3. View