Effect of Smilax Spp. and Phellinus Linteus Combination on Cytotoxicity and Cell Proliferation of Breast Cancer Cells

Overview

Journal BMC Complement Med Ther

Publisher Biomed Central

Specialty Rehabilitation Medicine

Date 2023 Jun 1

PMID 37264344

Authors

Kanwalat Chalertpet

Thanawitch Sangkheereeput

Prakaithip Somjit

Wichanee Bankeeree

Pattamawadee Yanatatsaneejit

Affiliations

Soon will be listed here.

Abstract

Background: Although the prevalence of breast cancer (BC) has been reduced in recent years, proficient therapeutic regimens should be further investigated with the aim of further reducing the mortality rate. To obtain more effective treatment, the present study aimed to observe the effects of PL synergistically combined with Smilax corbularia and S. glabra extracts (PSS) on BC cell lines, MCF7, T47D, MDA-MB-231, and MDA-MB-468.

Methods: The half-maximal inhibition (IC) concentrations of PSS and PL were determined in a dose- and time-dependent manner using MTT assay. The activity of PSS and PL on anti-BC proliferation was evaluated using BrdU assay, and colony formation assay. Moreover, cell cycle analysis and apoptosis induction as a result of PSS and PL exposure were investigated using propidium iodide (PI) staining and co-staining of annexin V DY634 and PI combined flow cytometric analysis, respectively. Finally, changes in the mRNA expression of genes involved in proliferative and apoptotic pathways (MKI67, HER2, EGFR, MDM2, TNFα, PI3KCA, KRAS, BAX, and CASP8) were explored using RT-qPCR following PSS and PL treatment.

Results: The PSS and PL extracts exhibited significant potential in BC cytotoxicity which were in were in dose- and time-dependent response. This inhibition of cell growth was due to the suppression of cell proliferation, the cell cycle arrest, and the induction of apoptosis. Additionally, an investigation of the underlying molecular mechanism revealed that PSS and PL are involved in downregulation of the MKI67, HER2, EGFR, MDM2, TNFα, and PI3KCA expression.

Conclusions: This present study has suggested that PSS and PL possess anti-BC proliferative activity mediated via the downregulation of genes participating in the relevant pathways. PSS or PL may be combined with other agents to alleviate the adverse side effects resulted from conventional chemotherapeutic drugs.

Citing Articles

A review on the cultivation, bioactive compounds, health-promoting factors and clinical trials of medicinal mushrooms Taiwanofungus camphoratus, Inonotus obliquus and Tropicoporus linteus.

Tee P, Krishnan T, Cheong X, Maniam S, Looi C, Ooi Y Fungal Biol Biotechnol. 2024; 11(1):7.

PMID: 38987829 PMC: 11238383. DOI: 10.1186/s40694-024-00176-3.

antioxidant and anticancer activities of extract combined with extract against breast cancer cell lines.

Syukriya A, Bankeeree W, Prasongsuk S, Yanatatsaneejit P Biomed Rep. 2023; 19(3):63.

PMID: 37614981 PMC: 10442767. DOI: 10.3892/br.2023.1645.

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