Antioxidant Capacity and Cytotoxic Effect of an Optimized Extract of Isabella Grape () on Breast Cancer Cells

Overview

Journal Heliyon

Specialty Social Sciences

Date 2023 Jun 1

PMID 37260897

Authors

M Daniela Velez

Maria A Llano-Ramirez

Carolina Ramon

Jessica Rojas

Carolina Bedoya

Sandra Arango-Varela

Gloria A Santa-Gonzalez

Maritza Gil

Affiliations

Soon will be listed here.

Abstract

The phenolic profile of Isabella grape () offers beneficial properties to human health and makes it a functional food product. In order to better understand the phenolic compounds found in this grape variety and the biological effect they induce on breast cancer cells, an ultrasound-assisted extraction was carried out. During the extraction of polyphenols from Isabella grapes organically grown in Antioquia (Colombia), parameters such as frequency (33 kHz and 40 kHz), time and solvent were optimized to finally obtain a crude extract with antioxidant properties (Oxygen Radical Absorbance Capacity, ORAC: 293.22 ± 34.73 μmol of Trolox/g of sample), associated with a total polyphenol content (TPC) of 43.14 ± 5.00 mg GAE/g sample and a total anthocyanin content composed of 17.69 ± 2.59 mg of malvidin-3-glucoside/100 g of sample. MCF-7 breast cancer cells were treated with different concentrations of the optimized extract, and results show a decrease in cell viability related to mitochondrial membrane depolarization, ROS increase, and chromatin condensation. To determine the possible death induction mechanism, molecular docking was simulated to predict the molecular interactions between the most abundant phenolic compounds in Isabella grape and the main apoptosis-related proteins. The results obtained from and experiments were consistent with each other, suggesting that the phenolic compounds found in Isabella grape can be considered potential adjuvant chemopreventive agents for the treatment of breast cancer.

Citing Articles

Antioxidant and Anticancer Activity of Extracts in Breast Cell Lines.

Tsantila E, Esslinger N, Christou M, Papageorgis P, Neophytou C Life (Basel). 2024; 14(2).

PMID: 38398737 PMC: 10890198. DOI: 10.3390/life14020228.

References

Crowley L, Marfell B, Waterhouse N . Analyzing Cell Death by Nuclear Staining with Hoechst 33342. Cold Spring Harb Protoc. 2016; 2016(9). DOI: 10.1101/pdb.prot087205. View

Schlachterman A, Valle F, Wall K, Azios N, Castillo L, Morell L . Combined resveratrol, quercetin, and catechin treatment reduces breast tumor growth in a nude mouse model. Transl Oncol. 2008; 1(1):19-27. PMC: 2510765. DOI: 10.1593/tlo.07100. View

AlYammahi J, Hai A, Krishnamoorthy R, Arumugham T, Hasan S, Banat F . Ultrasound-assisted extraction of highly nutritious date sugar from date palm (Phoenix dactylifera) fruit powder: Parametric optimization and kinetic modeling. Ultrason Sonochem. 2022; 88:106107. PMC: 9356216. DOI: 10.1016/j.ultsonch.2022.106107. View

Krych J, Gebicka L . Catalase is inhibited by flavonoids. Int J Biol Macromol. 2013; 58:148-53. DOI: 10.1016/j.ijbiomac.2013.03.070. View

Maldonado-Celisa M, Roussia S, Foltzer-Jourdainne C, Gosse F, Lobstein A, Habold C . Modulation by polyamines of apoptotic pathways triggered by procyanidins in human metastatic SW620 cells. Cell Mol Life Sci. 2008; 65(9):1425-34. PMC: 11131771. DOI: 10.1007/s00018-008-8023-4. View

Gonzalez-Centeno M, Knoerzer K, Sabarez H, Simal S, Rossello C, Femenia A . Effect of acoustic frequency and power density on the aqueous ultrasonic-assisted extraction of grape pomace (Vitis vinifera L.) - a response surface approach. Ultrason Sonochem. 2014; 21(6):2176-84. DOI: 10.1016/j.ultsonch.2014.01.021. View

Singletary K, Jung K, Giusti M . Anthocyanin-rich grape extract blocks breast cell DNA damage. J Med Food. 2007; 10(2):244-51. DOI: 10.1089/jmf.2006.258. View

de Souza V, Fujita A, Thomazini M, da Silva E, Lucon Jr J, Genovese M . Functional properties and stability of spray-dried pigments from Bordo grape (Vitis labrusca) winemaking pomace. Food Chem. 2014; 164:380-6. DOI: 10.1016/j.foodchem.2014.05.049. View

Silva F, de Cassia Ramos do Egypto Queiroga R, de Souza E, Voss G, da Silva Campelo Borges G, Dos Santos Lima M . Incorporation of phenolic-rich ingredients from integral valorization of Isabel grape improves the nutritional, functional and sensory characteristics of probiotic goat milk yogurt. Food Chem. 2021; 369:130957. DOI: 10.1016/j.foodchem.2021.130957. View

10.

Kushwaha P, Vardhan P, Kapewangolo P, Shuaib M, Prajapati S, Singh A . Bulbine frutescens phytochemical inhibits notch signaling pathway and induces apoptosis in triple negative and luminal breast cancer cells. Life Sci. 2019; 234:116783. DOI: 10.1016/j.lfs.2019.116783. View

11.

Paul S, Chakrabarty S, Ghosh S, Nag D, Das A, Dastidar D . Targeting cellular microtubule by phytochemical apocynin exhibits autophagy-mediated apoptosis to inhibit lung carcinoma progression and tumorigenesis. Phytomedicine. 2019; 67:153152. DOI: 10.1016/j.phymed.2019.153152. View

12.

Alkahtani H, Alanazi M, Aleanizy F, Alqahtani F, Alhoshani A, Alanazi F . Synthesis, anticancer, apoptosis-inducing activities and EGFR and VEGFR2 assay mechanistic studies of 5,5-diphenylimidazolidine-2,4-dione derivatives: Molecular docking studies. Saudi Pharm J. 2019; 27(5):682-693. PMC: 6598223. DOI: 10.1016/j.jsps.2019.04.003. View

13.

Fu J, Zhang L, Li W, Zhang Y, Zhang Y, Liu F . Application of metabolomics for revealing the interventional effects of functional foods on metabolic diseases. Food Chem. 2021; 367:130697. DOI: 10.1016/j.foodchem.2021.130697. View

14.

DAbrosca B, Lavorgna M, Scognamiglio M, Russo C, Graziani V, Piscitelli C . 2D-NMR investigation and in vitro evaluation of antioxidant, antigenotoxic and estrogenic/antiestrogenic activities of strawberry grape. Food Chem Toxicol. 2017; 105:52-60. DOI: 10.1016/j.fct.2017.03.050. View

15.

Nandi A, Yan L, Jana C, Das N . Role of Catalase in Oxidative Stress- and Age-Associated Degenerative Diseases. Oxid Med Cell Longev. 2019; 2019:9613090. PMC: 6885225. DOI: 10.1155/2019/9613090. View

16.

Das A, Majumder D, Saha C . Correlation of binding efficacies of DNA to flavonoids and their induced cellular damage. J Photochem Photobiol B. 2017; 170:256-262. DOI: 10.1016/j.jphotobiol.2017.04.019. View

17.

Deng G, Xu D, Li S, Li H . Optimization of Ultrasound-Assisted Extraction of Natural Antioxidants from Sugar Apple (Annona squamosa L.) Peel Using Response Surface Methodology. Molecules. 2015; 20(11):20448-59. PMC: 6332377. DOI: 10.3390/molecules201119708. View

18.

Lin D, Xiao M, Zhao J, Li Z, Xing B, Li X . An Overview of Plant Phenolic Compounds and Their Importance in Human Nutrition and Management of Type 2 Diabetes. Molecules. 2016; 21(10). PMC: 6274266. DOI: 10.3390/molecules21101374. View

19.

Helwa A, El-Dydamony N, A Radwan R, Abdelraouf S, Abdelnaby R . Novel antiproliferative agents bearing morpholinopyrimidine scaffold as PI3K inhibitors and apoptosis inducers; design, synthesis and molecular docking. Bioorg Chem. 2020; 102:104051. DOI: 10.1016/j.bioorg.2020.104051. View

20.

Boland M, Chourasia A, Macleod K . Mitochondrial dysfunction in cancer. Front Oncol. 2013; 3:292. PMC: 3844930. DOI: 10.3389/fonc.2013.00292. View