Ileocolonic Healing After Small Ileocecal Resection in Mice: NOD2 Deficiency Impairs Anastomotic Healing by Local Mechanisms

Overview

Journal J Clin Med

Specialty General Medicine

Date 2023 May 27

PMID 37240707

Authors

Maria B Witte

Johannes Saupe

Johannes Reiner

Karen Bannert

Clemens Schafmayer

Georg Lamprecht

Peggy Berlin

Affiliations

Soon will be listed here.

Abstract

Ileocecal resection (ICR) is frequently performed in Crohn's disease (CD). mutations are risk factors for CD. knockout (ko) mice show impaired anastomotic healing after extended ICR. We further investigated the role of NOD2 after limited ICR. C57B16/J (wt) and ko littermates underwent limited ICR including 1-2 cm terminal ileum and were randomly assigned to vehicle or MDP treatment. Bursting pressure was measured on POD 5, and the anastomosis was analyzed for matrix turn-over and granulation tissue. Wound fibroblasts from subcutaneously implanted sponges were used for comparison. The M1/M2 macrophage plasma cytokines were analyzed. Mortality was not different between groups. Bursting pressure was significantly decreased in ko mice. This was associated with less granulation tissue but was not affected by MDP. However, anastomotic leak (AL) rate tended to be lower in MDP-treated ko mice (29% vs. 11%, = 0.07). mRNA expression of , , () and was increased in ko mice, indicating increased matrix turn-over, specifically in the anastomosis. Systemic TNF-α expression was significantly lower in ko mice. Ileocolonic healing is impaired in ko mice after limited ICR by local mechanisms maybe including local dysbiosis.

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