Knockout of and in Medaka () Reveals Their Roles in Regulating Vision-Guided Behavior and Eye Development

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 May 27

PMID 37240129

Authors

Ke Lu

Jiaqi Wu

Shulin Tang

Xiaodan Jia

Xu-Fang Liang

Affiliations

Soon will be listed here.

Abstract

The medaka () is an excellent vertebrate model for studying the development of the retina. Its genome database is complete, and the number of opsin genes is relatively small compared to zebrafish. Short wavelength sensitive 2 (), a G-protein-coupled receptor expressed in the retina, has been lost in mammals, but its role in eye development in fish is still poorly understood. In this study, we established a and knockout medaka model by CRISPR/Cas9 technology. We discovered that medaka and are mainly expressed in the eyes and may be regulated by growth differentiation factor 6a (). Compared with the WT, and mutant larvae displayed an increase in swimming speed during the changes from light to dark. We also observed that and larvae both swam faster than WT in the first 10 s of the 2 min light period. The enhanced vision-guided behavior in and medaka larvae may be related to the upregulation of phototransduction-related genes. Additionally, we also found that affects the expression of eye development genes, while is unaffected. Together, these findings indicate that and knockouts increase vision-guided behavior and phototransduction, but on the other hand, plays an important role in regulating eye development genes. This study provides data for further understanding of the role of and in medaka retina development.

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