Omics Analysis Unveils the Pathway Involved in the Anthocyanin Biosynthesis in Tomato Seedling and Fruits

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 May 27

PMID 37240046

Authors

Rui He

Kaizhe Liu

Shuchang Zhang

Jun Ju

Youzhi Hu

Yamin Li

Xiaojuan Liu

Houcheng Liu

Affiliations

Soon will be listed here.

Abstract

The purple tomato variety 'Indigo Rose' (InR) is favored due to its bright appearance, abundant anthocyanins and outstanding antioxidant capacity. is associated with anthocyanin biosynthesis in 'Indigo Rose' plants. However, residual anthocyanins still present in seedlings and fruit peel indicated there was an anthocyanin induction pathway that is independent of HY5 in plants. The molecular mechanism of anthocyanins formation in 'Indigo Rose' and mutants is unclear. In this study, we performed omics analysis to clarify the regulatory network underlying anthocyanin biosynthesis in seedling and fruit peel of 'Indigo Rose' and mutant. Results showed that the total amount of anthocyanins in both seedling and fruit of InR was significantly higher than those in the mutant, and most genes associated with anthocyanin biosynthesis exhibited higher expression levels in InR, suggesting that play pivotal roles in flavonoid biosynthesis both in tomato seedlings and fruit. Yeast two-hybrid (Y2H) results revealed that physically interacts with and , while could interact with protein. Unexpectedly, both and were found to interact with , and by yeast two-hybrid assay. Suppression of by virus-induced gene silencing (VIGS) retarded the purple coloration of the fruit peel, indicating an important role of in the regulation of anthocyanin accumulation. These results deepen the understanding of purple color formation in tomato seedlings and fruits in an -dependent or independent manner via excavating the genes involved in anthocyanin biosynthesis based on omics analysis.

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