Enhanced Resistance of Against Pv. Suggests Negative Regulation of Plant Basal Defense and Systemic Acquired Resistance by Encoding NAD(P)-Binding Rossmann-Fold in

Overview

Journal Antioxidants (Basel)

Date 2023 May 27

PMID 37237855

Authors

Tiba Nazar Al Azzawi

Murtaza Khan

Bong-Gyu Mun

Sang-Uk Lee

Muhammad Imran

Adil Hussain

Nkulu Kabange Rolly

Da-Sol Lee

Sajid Ali

In-Jung Lee

Byung-Wook Yun

Affiliations

Soon will be listed here.

Abstract

Nitric oxide (NO) regulates several biological and physiological processes in plants. This study investigated the role of (), encoding an NAD(P)-binding Rossmann-fold superfamily, in the growth and immunity of . was pooled from the CySNO transcriptome as a NO-responsive gene. Seeds of the knockout () and overexpression plants were evaluated for their response to oxidative [(hydrogen peroxide (HO) and methyl viologen (MV)] or nitro-oxidative [(S-nitroso-L-cysteine (CySNO) and S-nitroso glutathione (GSNO)] stress. Results showed that the root and shoot growth of (KO) and (OE) exhibited differential phenotypic responses under oxidative and nitro-oxidative stress and normal growth conditions. To investigate the role of the target gene in plant immunity, the biotrophic bacterial pathogen pv. DC3000 virulent ( DC3000 ) was used to assess the basal defense, while the DC3000 avirulent (B) strain was used to investigate -gene-mediated resistance and systemic acquired resistance (SAR). Data revealed that negatively regulated basal defense, -gene-mediated resistance, and SAR. Furthermore, the eFP browser indicated that the expression of is detected in several plant organs, with the highest expression observed in germinating seeds. All results put together suggest that could be involved in plant growth, as well as basal defense and SAR, in response to bacterial pathogens in .

References

Hussain A, Mun B, Imran Q, Lee S, Adamu T, Shahid M . Nitric Oxide Mediated Transcriptome Profiling Reveals Activation of Multiple Regulatory Pathways in Arabidopsis thaliana. Front Plant Sci. 2016; 7:975. PMC: 4926318. DOI: 10.3389/fpls.2016.00975. View

Khan M, Ali S, Azzawi T, Saqib S, Ullah F, Ayaz A . The Key Roles of ROS and RNS as a Signaling Molecule in Plant-Microbe Interactions. Antioxidants (Basel). 2023; 12(2). PMC: 9952064. DOI: 10.3390/antiox12020268. View

Letunic I, Khedkar S, Bork P . SMART: recent updates, new developments and status in 2020. Nucleic Acids Res. 2020; 49(D1):D458-D460. PMC: 7778883. DOI: 10.1093/nar/gkaa937. View

Khan M, Al Azawi T, Pande A, Mun B, Lee D, Hussain A . The Role of Nitric Oxide-Induced in Growth and Disease Resistance in . Front Plant Sci. 2021; 12:685156. PMC: 8285060. DOI: 10.3389/fpls.2021.685156. View

Tuteja N, Chandra M, Tuteja R, Misra M . Nitric Oxide as a Unique Bioactive Signaling Messenger in Physiology and Pathophysiology. J Biomed Biotechnol. 2004; 2004(4):227-237. PMC: 555773. DOI: 10.1155/S1110724304402034. View

Syed Nabi R, Tayade R, Imran Q, Hussain A, Shahid M, Yun B . Functional Insight of Nitric-Oxide Induced DUF Genes in . Front Plant Sci. 2020; 11:1041. PMC: 7378322. DOI: 10.3389/fpls.2020.01041. View

Yun B, Feechan A, Yin M, Saidi N, Le Bihan T, Yu M . S-nitrosylation of NADPH oxidase regulates cell death in plant immunity. Nature. 2011; 478(7368):264-8. DOI: 10.1038/nature10427. View

Xue Y, Liu Z, Gao X, Jin C, Wen L, Yao X . GPS-SNO: computational prediction of protein S-nitrosylation sites with a modified GPS algorithm. PLoS One. 2010; 5(6):e11290. PMC: 2892008. DOI: 10.1371/journal.pone.0011290. View

Khan M, Imran Q, Shahid M, Mun B, Lee S, Khan M . Nitric oxide- induced AtAO3 differentially regulates plant defense and drought tolerance in Arabidopsis thaliana. BMC Plant Biol. 2020; 19(1):602. PMC: 6937950. DOI: 10.1186/s12870-019-2210-3. View

10.

Pande A, Mun B, Lee D, Khan M, Lee G, Hussain A . NO Network for Plant-Microbe Communication Underground: A Review. Front Plant Sci. 2021; 12:658679. PMC: 8010196. DOI: 10.3389/fpls.2021.658679. View

11.

Hussain A, Yun B, Kim J, Gupta K, Hyung N, Loake G . Novel and conserved functions of S-nitrosoglutathione reductase in tomato. J Exp Bot. 2019; 70(18):4877-4886. PMC: 6760305. DOI: 10.1093/jxb/erz234. View

12.

Huang X, Von Rad U, Durner J . Nitric oxide induces transcriptional activation of the nitric oxide-tolerant alternative oxidase in Arabidopsis suspension cells. Planta. 2002; 215(6):914-23. DOI: 10.1007/s00425-002-0828-z. View

13.

Muday G, Brown-Harding H . Nervous system-like signaling in plant defense. Science. 2018; 361(6407):1068-1069. DOI: 10.1126/science.aau9813. View

14.

Rolly N, Imran Q, Shahid M, Imran M, Khan M, Lee S . Drought-induced AtbZIP62 transcription factor regulates drought stress response in Arabidopsis. Plant Physiol Biochem. 2020; 156:384-395. DOI: 10.1016/j.plaphy.2020.09.013. View

15.

Bari R, Jones J . Role of plant hormones in plant defence responses. Plant Mol Biol. 2008; 69(4):473-88. DOI: 10.1007/s11103-008-9435-0. View

16.

Ali M, Kim I, Bilal S, Shahzad R, Saeed M, Adhikari B . Effects of Bacterial Fermentation on the Biochemical Constituents and Antioxidant Potential of Fermented and Unfermented Soybeans Using Probiotic Bacillus subtilis (KCTC 13241). Molecules. 2017; 22(12). PMC: 6149969. DOI: 10.3390/molecules22122200. View

17.

Kim Y, Park S, Gilmour S, Thomashow M . Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis. Plant J. 2013; 75(3):364-76. DOI: 10.1111/tpj.12205. View

18.

Gao Z, Wang Y, Chen G, Zhang A, Yang S, Shang L . The indica nitrate reductase gene OsNR2 allele enhances rice yield potential and nitrogen use efficiency. Nat Commun. 2019; 10(1):5207. PMC: 6858341. DOI: 10.1038/s41467-019-13110-8. View

19.

Turkan I . ROS and RNS: key signalling molecules in plants. J Exp Bot. 2018; 69(14):3313-3315. PMC: 6009602. DOI: 10.1093/jxb/ery198. View

20.

Letunic I, Bork P . 20 years of the SMART protein domain annotation resource. Nucleic Acids Res. 2017; 46(D1):D493-D496. PMC: 5753352. DOI: 10.1093/nar/gkx922. View