Pulp-Dentin Complex Regeneration with Cell Transplantation Technique Using Stem Cells Derived from Human Deciduous Teeth: Histological and Immunohistochemical Study in Immunosuppressed Rats

Overview

Journal Bioengineering (Basel)

Date 2023 May 27

PMID 37237680

Authors

Larissa Regina Kuntze Dos Santos

Andre Antonio Pelegrine

Carlos Eduardo da Silveira Bueno

Jose Ricardo Muniz Ferreira

Antonio Carlos Aloise

Carolina Pessoa Stringheta

Elizabeth Ferreira Martinez

Rina Andrea Pelegrine

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to histologically verify the performance of pulp-derived stem cells used in the pulp-dentin complex regeneration. Maxillary molars of 12 immunosuppressed rats were divided into two groups: the SC (stem cells) group, and the PBS (just standard phosphate-buffered saline) group. After pulpectomy and canal preparation, the teeth received the designated materials, and the cavities were sealed. After 12 weeks, the animals were euthanized, and the specimens underwent histological processing and qualitative evaluation of intracanal connective tissue, odontoblast-like cells, intracanal mineralized tissue, and periapical inflammatory infiltrate. Immunohistochemical evaluation was performed to detect dentin matrix protein 1 (DMP1). In the PBS group, an amorphous substance and remnants of mineralized tissue were observed throughout the canal, and abundant inflammatory cells were observed in the periapical region. In the SC group, an amorphous substance and remnants of mineralized tissue were observed throughout the canal; odontoblasts-like cells immunopositive for DMP1 and mineral plug were observed in the apical region of the canal; and a mild inflammatory infiltrate, intense vascularization, and neoformation of organized connective tissue were observed in the periapical region. In conclusion, the transplantation of human pulp stem cells promoted partial pulp tissue neoformation in adult rat molars.

Citing Articles

Conditioned Media from Human Pulp Stem Cell Cultures Improve Bone Regeneration in Rat Calvarial Critical-Size Defects.

Buss L, de Martin G, Martinez E, Filgueiras I, Magnabosco J, Alves B J Funct Biomater. 2023; 14(8).

PMID: 37623641 PMC: 10455841. DOI: 10.3390/jfb14080396.

References

Berthiaume F, Maguire T, Yarmush M . Tissue engineering and regenerative medicine: history, progress, and challenges. Annu Rev Chem Biomol Eng. 2012; 2:403-30. DOI: 10.1146/annurev-chembioeng-061010-114257. View

Souron J, Petiet A, Decup F, Tran X, Lesieur J, Poliard A . Pulp cell tracking by radionuclide imaging for dental tissue engineering. Tissue Eng Part C Methods. 2013; 20(3):188-97. PMC: 3936500. DOI: 10.1089/ten.TEC.2013.0148. View

Nishimura Y, Aida T, Taguchi Y . Advances in tissue engineering technology for kidney regeneration and construction. J Artif Organs. 2022; 25(3):191-194. DOI: 10.1007/s10047-022-01315-6. View

Fisher M, Belkin N, Milby A, Henning E, Bostrom M, Kim M . Cartilage repair and subchondral bone remodeling in response to focal lesions in a mini-pig model: implications for tissue engineering. Tissue Eng Part A. 2014; 21(3-4):850-60. PMC: 4333259. DOI: 10.1089/ten.TEA.2014.0384. View

de Oliveira T, Aloise A, Orosz J, de Mello e Oliveira R, de Carvalho P, Pelegrine A . Double Centrifugation Versus Single Centrifugation of Bone Marrow Aspirate Concentrate in Sinus Floor Elevation: A Pilot Study. Int J Oral Maxillofac Implants. 2016; 31(1):216-22. DOI: 10.11607/jomi.4170. View

Yu J, Wang Y, Deng Z, Tang L, Li Y, Shi J . Odontogenic capability: bone marrow stromal stem cells versus dental pulp stem cells. Biol Cell. 2007; 99(8):465-74. DOI: 10.1042/BC20070013. View

Chen H, Fu H, Wu X, Duan Y, Zhang S, Hu H . Regeneration of pulpo-dentinal-like complex by a group of unique multipotent CD24a stem cells. Sci Adv. 2020; 6(15):eaay1514. PMC: 7141825. DOI: 10.1126/sciadv.aay1514. View

Ostrovidov S, Salehi S, Costantini M, Suthiwanich K, Ebrahimi M, Banan Sadeghian R . 3D Bioprinting in Skeletal Muscle Tissue Engineering. Small. 2019; 15(24):e1805530. PMC: 6570559. DOI: 10.1002/smll.201805530. View

Nakashima M, Iohara K . Mobilized dental pulp stem cells for pulp regeneration: initiation of clinical trial. J Endod. 2014; 40(4 Suppl):S26-32. DOI: 10.1016/j.joen.2014.01.020. View

10.

Wubneh A, Tsekoura E, Ayranci C, Uludag H . Current state of fabrication technologies and materials for bone tissue engineering. Acta Biomater. 2018; 80:1-30. DOI: 10.1016/j.actbio.2018.09.031. View

11.

Shaikh M, Shahzad Z, Tash E, Janjua O, Khan M, Zafar M . Human Umbilical Cord Mesenchymal Stem Cells: Current Literature and Role in Periodontal Regeneration. Cells. 2022; 11(7). PMC: 8997495. DOI: 10.3390/cells11071168. View

12.

Smith A, Duncan H, Diogenes A, Simon S, Cooper P . Exploiting the Bioactive Properties of the Dentin-Pulp Complex in Regenerative Endodontics. J Endod. 2015; 42(1):47-56. DOI: 10.1016/j.joen.2015.10.019. View

13.

Correa S, Elias de Sousa J, Pasquali P, de Macedo L, Aloise A, Teixeira M . Use of Bone Allograft With or Without Bone Marrow Aspirate Concentrate in Appositional Reconstructions: A Tomographic and Histomorphometric Study. Implant Dent. 2017; 26(6):915-921. DOI: 10.1097/ID.0000000000000669. View

14.

Galler K, Widbiller M, Buchalla W, Eidt A, Hiller K, Hoffer P . EDTA conditioning of dentine promotes adhesion, migration and differentiation of dental pulp stem cells. Int Endod J. 2015; 49(6):581-90. DOI: 10.1111/iej.12492. View

15.

Versiani M, Alves F, Andrade-Junior C, Marceliano-Alves M, Provenzano J, Rocas I . Micro-CT evaluation of the efficacy of hard-tissue removal from the root canal and isthmus area by positive and negative pressure irrigation systems. Int Endod J. 2015; 49(11):1079-1087. DOI: 10.1111/iej.12559. View

16.

Zhu X, Zhang C, Huang G, Cheung G, Dissanayaka W, Zhu W . Transplantation of dental pulp stem cells and platelet-rich plasma for pulp regeneration. J Endod. 2012; 38(12):1604-9. DOI: 10.1016/j.joen.2012.09.001. View

17.

Lekhooa M, Walubo A, Du Plessis J, Matsabisa M . The development and use of a drug-induced immunosuppressed rat-model to screen Phela for mechanism of immune stimulation. J Ethnopharmacol. 2017; 206:8-18. DOI: 10.1016/j.jep.2017.04.031. View

18.

Nam H, Jeong H, Jo Y, Lee J, Ha D, Kim J . Multi-layered Free-form 3D Cell-printed Tubular Construct with Decellularized Inner and Outer Esophageal Tissue-derived Bioinks. Sci Rep. 2020; 10(1):7255. PMC: 7190629. DOI: 10.1038/s41598-020-64049-6. View

19.

Aloise A, Pelegrine A, Zimmermann A, de Mello e Oliveira R, Ferreira L . Repair of critical-size bone defects using bone marrow stem cells or autogenous bone with or without collagen membrane: a histomorphometric study in rabbit calvaria. Int J Oral Maxillofac Implants. 2015; 30(1):208-15. DOI: 10.11607/jomi.4010. View

20.

Jango H, Gras S, Christensen L, Lose G . Tissue-engineering with muscle fiber fragments improves the strength of a weak abdominal wall in rats. Int Urogynecol J. 2016; 28(2):223-229. DOI: 10.1007/s00192-016-3091-8. View