Screening Fungal Strains Isolated from a Limestone Cave on Their Ability to Grow and Precipitate CaCO in an Environment Relevant to Concrete

Overview

Journal MicroPubl Biol

Specialty Biology

Date 2023 May 24

PMID 37223428

Authors

Aurelie Van Wylick

Simon Vandersanden

Karl Jonckheere

Hubert Rahier

Lars De Laet

Eveline Peeters

Affiliations

Soon will be listed here.

Abstract

Fungi-mediated self-healing concrete is a novel approach that promotes the precipitation of calcium carbonate (CaCO ) on fungal hyphae to heal the cracks in concrete. In this study, we set out to explore the potential of fungal species isolated from a limestone cave by investigating their ability to precipitate CaCO and to survive and grow in conditions relevant to concrete. Isolated strains belonging to the genera , and proved to be promising candidates for fungi-mediated self-healing concrete attributed to their growth properties and CaCO precipitation capabilities in the presence of cement.

Citing Articles

A Review of the Biotechnological Potential of Cave Fungi: A Toolbox for the Future.

Barbosa R, Felipe M, Silva L, Silva E, Silva S, Herculano P J Fungi (Basel). 2025; 11(2).

PMID: 39997439 PMC: 11856267. DOI: 10.3390/jof11020145.

Conditions for CaCO Biomineralization by with the Perspective of Developing Fungi-Mediated Self-Healing Concrete.

Van Wylick A, Rahier H, De Laet L, Peeters E Glob Chall. 2024; 8(1):2300160.

PMID: 38223894 PMC: 10784186. DOI: 10.1002/gch2.202300160.

References

Li Q, Csetenyi L, Gadd G . Biomineralization of metal carbonates by Neurospora crassa. Environ Sci Technol. 2014; 48(24):14409-16. DOI: 10.1021/es5042546. View

Van Wylick A, Monclaro A, Elsacker E, Vandelook S, Rahier H, De Laet L . A review on the potential of filamentous fungi for microbial self-healing of concrete. Fungal Biol Biotechnol. 2021; 8(1):16. PMC: 8600713. DOI: 10.1186/s40694-021-00122-7. View

Fahimizadeh M, Diane Abeyratne A, Mae L, Singh R, Pasbakhsh P . Biological Self-Healing of Cement Paste and Mortar by Non-Ureolytic Bacteria Encapsulated in Alginate Hydrogel Capsules. Materials (Basel). 2020; 13(17). PMC: 7504608. DOI: 10.3390/ma13173711. View

Tastan B . Clean up fly ash from coal burning plants by new isolated fungi Fusarium oxysporum and Penicillium glabrum. J Environ Manage. 2017; 200:46-52. DOI: 10.1016/j.jenvman.2017.05.062. View

CHRISTENSEN W . Urea Decomposition as a Means of Differentiating Proteus and Paracolon Cultures from Each Other and from Salmonella and Shigella Types. J Bacteriol. 1946; 52(4):461-6. PMC: 518212. DOI: 10.1128/jb.52.4.461-466.1946. View

Xie J, Strobel G, Xu W, Chen J, Ren H, An D . Fungi as Architects of the Rimstone Dams in Huanglong, NSD, Sichuan, China. Microb Ecol. 2016; 73(1):29-38. DOI: 10.1007/s00248-016-0841-6. View

Seifan M, Samani A, Berenjian A . Bioconcrete: next generation of self-healing concrete. Appl Microbiol Biotechnol. 2016; 100(6):2591-602. DOI: 10.1007/s00253-016-7316-z. View

Menon R, Luo J, Chen X, Zhou H, Liu Z, Zhou G . Screening of Fungi for Potential Application of Self-Healing Concrete. Sci Rep. 2019; 9(1):2075. PMC: 6375922. DOI: 10.1038/s41598-019-39156-8. View

Bellemain E, Carlsen T, Brochmann C, Coissac E, Taberlet P, Kauserud H . ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases. BMC Microbiol. 2010; 10:189. PMC: 2909996. DOI: 10.1186/1471-2180-10-189. View

10.

Rossi M, Oliveira V . Growth of the Ectomycorrhizal Fungus Pisolithus Microcarpus in different nutritional conditions. Braz J Microbiol. 2013; 42(2):624-32. PMC: 3769840. DOI: 10.1590/S1517-838220110002000027. View

11.

Park S, Cho Y, Jung D, Jo K, Lee E, Lee J . Microbial Diversity in Moonmilk of Baeg-nyong Cave, Korean CZO. Front Microbiol. 2020; 11:613. PMC: 7190796. DOI: 10.3389/fmicb.2020.00613. View