Assessing the Hepatoprotective Effects of Hesperidin on Liver-associated Disorders in Albino Rats with Experimentally Induced Obesity and Type II Diabetes: A Histological and Biochemical Study

Overview

Journal Heliyon

Specialty Social Sciences

Date 2023 May 22

PMID 37215885

Authors

Ihab Shafek Atta

Mohamed R Elnady

Ali G Alghamdi

Ahmed Hassan Alghamdi

Alaa A Aboulata

Ibrahim M Shatla

Affiliations

Soon will be listed here.

Abstract

Aim: Determination of curative effects of HSP on the liver in T2DM rats through biochemical and histopathological studies.

Methods: Animals. Fifty rats were enrolled. 10 rats were fed a normal diet (control group), and the remaining 40 rats received a high-fat diet (HFD) for 8 weeks. The HFD-fed rats were grouped into Group II: 10 rats, and Group III: 10 rats received HSP 100 mg/kg. Group IV: 10 rats received a single dose of streptozotocin (STZ), 30 mg/kg, and Group V: 10 rats received STZ and HSP. Body weight, Blood glucose, insulin level, liver enzymes, lipid profile, oxidative stress, TNF-α, NF-κB, and liver biopsy were estimated.

Results: there is improvement in the histological profile of the steatosis in HFD-fed rats treated with HSP either in group III or in group V (received STZ) along with amelioration in blood glucose, insulin, liver enzymes, lipid profile, oxidative profile, TNF-α, and NF-κB.

Conclusion: HSP in this STZ model revealed an improvement in steatosis, biochemical markers, and histologic findings. By studying these factors, we expected to identify the prospective targets for intervention that could help improve outcomes for individuals with obesity and diabetes-related liver diseases.

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References

Lee J, Won Jun D, Kim E, Jeon H, Nam H, Saeed W . Histologic and Metabolic Derangement in High-Fat, High-Fructose, and Combination Diet Animal Models. ScientificWorldJournal. 2015; 2015:306326. PMC: 4451512. DOI: 10.1155/2015/306326. View

El-Shahawy A, Abdel-Moneim A, Ebeid A, Eldin Z, Zanaty M . A novel layered double hydroxide-hesperidin nanoparticles exert antidiabetic, antioxidant and anti-inflammatory effects in rats with diabetes. Mol Biol Rep. 2021; 48(6):5217-5232. DOI: 10.1007/s11033-021-06527-2. View

Younossi Z, Koenig A, Abdelatif D, Fazel Y, Henry L, Wymer M . Global epidemiology of nonalcoholic fatty liver disease-Meta-analytic assessment of prevalence, incidence, and outcomes. Hepatology. 2015; 64(1):73-84. DOI: 10.1002/hep.28431. View

Williams R, Karuranga S, Malanda B, Saeedi P, Basit A, Besancon S . Global and regional estimates and projections of diabetes-related health expenditure: Results from the International Diabetes Federation Diabetes Atlas, 9th edition. Diabetes Res Clin Pract. 2020; 162:108072. DOI: 10.1016/j.diabres.2020.108072. View

Nishikimi M, Appaji N, Yagi K . The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res Commun. 1972; 46(2):849-54. DOI: 10.1016/s0006-291x(72)80218-3. View

Wang Y, Singh R, Lefkowitch J, Rigoli R, Czaja M . Tumor necrosis factor-induced toxic liver injury results from JNK2-dependent activation of caspase-8 and the mitochondrial death pathway. J Biol Chem. 2006; 281(22):15258-67. PMC: 3668334. DOI: 10.1074/jbc.M512953200. View

Sharma B, Balomajumder C, Roy P . Hypoglycemic and hypolipidemic effects of flavonoid rich extract from Eugenia jambolana seeds on streptozotocin induced diabetic rats. Food Chem Toxicol. 2008; 46(7):2376-83. DOI: 10.1016/j.fct.2008.03.020. View

Galicia-Garcia U, Benito-Vicente A, Jebari S, Larrea-Sebal A, Siddiqi H, Uribe K . Pathophysiology of Type 2 Diabetes Mellitus. Int J Mol Sci. 2020; 21(17). PMC: 7503727. DOI: 10.3390/ijms21176275. View

Barriere D, Noll C, Roussy G, Lizotte F, Kessai A, Kirby K . Combination of high-fat/high-fructose diet and low-dose streptozotocin to model long-term type-2 diabetes complications. Sci Rep. 2018; 8(1):424. PMC: 5765114. DOI: 10.1038/s41598-017-18896-5. View

10.

Jung U, Lee M, Park Y, Kang M, Choi M . Effect of citrus flavonoids on lipid metabolism and glucose-regulating enzyme mRNA levels in type-2 diabetic mice. Int J Biochem Cell Biol. 2006; 38(7):1134-45. DOI: 10.1016/j.biocel.2005.12.002. View

11.

Gandhi G, Stalin A, Balakrishna K, Ignacimuthu S, Paulraj M, Vishal R . Insulin sensitization via partial agonism of PPARγ and glucose uptake through translocation and activation of GLUT4 in PI3K/p-Akt signaling pathway by embelin in type 2 diabetic rats. Biochim Biophys Acta. 2012; 1830(1):2243-55. DOI: 10.1016/j.bbagen.2012.10.016. View

12.

Pandey P, Sayyed U, Tiwari R, Siddiqui M, Pathak N, Bajpai P . Hesperidin Induces ROS-Mediated Apoptosis along with Cell Cycle Arrest at G2/M Phase in Human Gall Bladder Carcinoma. Nutr Cancer. 2018; 71(4):676-687. DOI: 10.1080/01635581.2018.1508732. View

13.

Esmail M, Anwar S, Kandeil M, El-Zanaty A, Abdel-Gabbar M . Effect of Nigella sativa, atorvastatin, or L-Carnitine on high fat diet-induced obesity in adult male Albino rats. Biomed Pharmacother. 2021; 141:111818. DOI: 10.1016/j.biopha.2021.111818. View

14.

Lee J, Han Y, Wang W, Jo H, Kim H, Kim S . Phytochemicals in Cancer Immune Checkpoint Inhibitor Therapy. Biomolecules. 2021; 11(8). PMC: 8393583. DOI: 10.3390/biom11081107. View

15.

Ohshima K, Mogi M, Jing F, Iwanami J, Tsukuda K, Min L . Roles of interleukin 17 in angiotensin II type 1 receptor-mediated insulin resistance. Hypertension. 2011; 59(2):493-9. DOI: 10.1161/HYPERTENSIONAHA.111.183178. View

16.

Qi W, Lin C, Fan K, Chen Z, Liu L, Feng X . Hesperidin inhibits synovial cell inflammation and macrophage polarization through suppression of the PI3K/AKT pathway in complete Freund's adjuvant-induced arthritis in mice. Chem Biol Interact. 2019; 306:19-28. DOI: 10.1016/j.cbi.2019.04.002. View

17.

Cao H, Hininger-Favier I, Kelly M, Benaraba R, Dawson H, Coves S . Green tea polyphenol extract regulates the expression of genes involved in glucose uptake and insulin signaling in rats fed a high fructose diet. J Agric Food Chem. 2007; 55(15):6372-8. DOI: 10.1021/jf070695o. View

18.

Wilcox L, Borradaile N, de Dreu L, Huff M . Secretion of hepatocyte apoB is inhibited by the flavonoids, naringenin and hesperetin, via reduced activity and expression of ACAT2 and MTP. J Lipid Res. 2001; 42(5):725-34. View

19.

Momen Y, Hussein R, Kandeil M . Involvement of PI3K/Akt pathway in the protective effect of hesperidin against a chemically induced liver cancer in rats. J Biochem Mol Toxicol. 2019; 33(6):e22305. DOI: 10.1002/jbt.22305. View

20.

Saeedi P, Petersohn I, Salpea P, Malanda B, Karuranga S, Unwin N . Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the International Diabetes Federation Diabetes Atlas, 9 edition. Diabetes Res Clin Pract. 2019; 157:107843. DOI: 10.1016/j.diabres.2019.107843. View