Effects of Different Fertilization Conditions and Different Geographical Locations on the Diversity and Composition of the Rhizosphere Microbiota of Qingke ( L.) Plants in Different Growth Stages

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2023 May 22

PMID 37213511

Authors

Lei Wang

Handong Wang

Meijin Liu

Jinqing Xu

Haiyan Bian

Tongrui Chen

En You

Chao Deng

Youhai Wei

Tianyu Yang

Yuhu Shen

Affiliations

Soon will be listed here.

Abstract

Introduction: The excessive use of chemical fertilizer causes increasing environmental and food security crisis. Organic fertilizer improves physical and biological activities of soil. Rhizosphere microbiota, which consist of highly diverse microorganisms, play an important role in soil quality. However, there is limited information about the effects of different fertilization conditions on the growth of Qingke plants and composition of the rhizosphere microbiota of the plants.

Methods: In this study, we characterized the rhizosphere microbiota of Qingke plants grown in three main Qingke-producing areas (Tibet, Qinghai, and Gansu). In each of the three areas, seven different fertilization conditions (m1-m7, m1: Unfertilized; m2: Farmer Practice; m3: 75% Farmer Practice; m4: 75% Farmer Practice +25% Organic manure; m5: 50% Farmer Practice; m6: 50% Farmer Practice +50% Organic manure; m7: 100% Organic manure) were applied. The growth and yields of the Qingke plants were also compared under the seven fertilization conditions.

Results: There were significant differences in alpha diversity indices among the three areas. In each area, differences in fertilization conditions and differences in the growth stages of Qingke plants resulted in differences in the beta diversity of the rhizosphere microbiota. Meanwhile, in each area, fertilization conditions, soil depths, and the growth stages of Qingke plants significantly affected the relative abundance of the top 10 phyla and the top 20 bacterial genera. For most of microbial pairs established through network analysis, the significance of their correlations in each of the microbial co-occurrence networks of the three experimental sites was different. Moreover, in each of the three networks, there were significant differences in relative abundance and genera among most nodes (i.e., the genera , , , , and ). The soil chemical properties (i.e., TN, TP, SOM, AN, AK, CEC, Ca, and K) were positively or negatively correlated with the relative abundance of the top 30 genera derived from the three main Qingke-producing areas ( < 0.05). Fertilization conditions markedly influenced the height of a Qingke plant, the number of spikes in a Qingke plant, the number of kernels in a spike, and the fresh weight of a Qingke plant. Considering the yield, the most effective fertilization conditions for Qingke is combining application 50% chemical fertilizer and 50% organic manure.

Conclusion: The results of the present study can provide theoretical basis for practice of reducing the use of chemical fertilizer in agriculture.

Citing Articles

Deciphering key factors in pathogen-suppressive microbiome assembly in the rhizosphere.

Andargie Y, Lee G, Jeong M, Tagele S, Shin J Front Plant Sci. 2023; 14:1301698.

PMID: 38116158 PMC: 10728675. DOI: 10.3389/fpls.2023.1301698.

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