A Portable Electrochemical Immunosensor for Ovarian Cancer Uses Hierarchical Microporous Carbon Material from Waste Coffee Grounds

Overview

Journal Mikrochim Acta

Specialties Biotechnology
Chemistry

Date 2023 May 22

PMID 37213023

Authors

Suparat Cotchim

Panote Thavarungkul

Proespichaya Kanatharana

Thaweesak Thantipwan

Amorn Jiraseree-Amornkun

Rodtichoti Wannapob

Warakorn Limbut

Affiliations

Soon will be listed here.

Abstract

A simple label-free electrochemical immunosensor for ovarian cancer (OC) detection was developed using a hierarchical microporous carbon material fabricated from waste coffee grounds (WCG). The analysis method exploited near-field communication (NFC) and a smartphone-based potentiostat. Waste coffee grounds were pyrolyzed with potassium hydroxide and used to modify a screen-printed electrode. The modified screen-printed electrode was decorated with gold nanoparticles (AuNPs) to capture a specific antibody. The modification and immobilization processes were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The sensor had an effective dynamic range of 0.5 to 50.0 U mL of cancer antigen 125 (CA125) tumor marker with a correlation coefficient of 0.9995. The limit of detection (LOD) was 0.4 U mL. A comparison of the results obtained from human serum analysis with the proposed immunosensor and the results obtained from the clinical method confirmed the accuracy and precision of the proposed immunosensor.

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