Incidence of Methanogenic Bacteria in a Sigmoidoscopy Population: an Association of Methanogenic Bacteria and Diverticulosis

Overview

Journal Gut

Specialty Gastroenterology

Date 1986 Jun 1

PMID 3721294

Citations 67

Authors

G A Weaver

J A Krause

T L Miller

M J Wolin

Affiliations

Soon will be listed here.

Abstract

This study determined the incidence and concentration of methane-producing bacteria in tap water enema samples of 130 individuals taken before sigmoidoscopy. The number of subjects classified in five major colonic groups were as follows: normal colon 36, diverticulosis 57, inflammatory bowel disease 11, colon polyps 34, and colon cancer 11. Some patients were placed in more than one category. Ninety four of the subjects or 72% had methanogenic bacteria ranging in concentration from 6 to about 3 X 10(10)/g dry weight of faeces. The predominant methanogen in all groups was Methanobrevibacter smithii. Chi-square analysis showed that the incidence of methanogens in concentrations of 10(7)/g dry weight of faeces or greater in patients with diverticulosis (58%) was significantly greater than in normal patients (25%). High methanogen concentrations are associated with excretion of methane in the breath.

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References

Bond Jr J, Engel R, Levitt M . Factors influencing pulmonary methane excretion in man. An indirect method of studying the in situ metabolism of the methane-producing colonic bacteria. J Exp Med. 1971; 133(3):572-88. PMC: 2138941. DOI: 10.1084/jem.133.3.572. View

McKay L, Eastwood M, Brydon W . Methane excretion in man--a study of breath, flatus, and faeces. Gut. 1985; 26(1):69-74. PMC: 1432392. DOI: 10.1136/gut.26.1.69. View

Haines A, Metz G, Dilawari J, Blendis L, Wiggins H . Breath-methane in patients with cancer of the large bowel. Lancet. 1977; 2(8036):481-3. DOI: 10.1016/s0140-6736(77)91605-1. View

Tadesse K, Eastwood M . Metabolism of dietary fibre components in man assessed by breath hydrogen and methane. Br J Nutr. 1978; 40(2):393-6. DOI: 10.1079/bjn19780136. View

Niu H, Schoeller D, KLEIN P . Improved gas chromatographic quantitation of breath hydrogen by normalization to respiratory carbon dioxide. J Lab Clin Med. 1979; 94(5):755-63. View

Balch W, Fox G, Magrum L, Woese C, WOLFE R . Methanogens: reevaluation of a unique biological group. Microbiol Rev. 1979; 43(2):260-96. PMC: 281474. DOI: 10.1128/mr.43.2.260-296.1979. View

Pitt P, de Bruijn K, Beeching M, Goldberg E, Blendis L . Studies on breath methane: the effect of ethnic origins and lactulose. Gut. 1980; 21(11):951-4. PMC: 1419292. DOI: 10.1136/gut.21.11.951. View

Wolin M . Fermentation in the rumen and human large intestine. Science. 1981; 213(4515):1463-8. DOI: 10.1126/science.7280665. View

McKay L, Brydon W, Eastwood M, Smith J . The influence of pentose on breath methane. Am J Clin Nutr. 1981; 34(12):2728-33. DOI: 10.1093/ajcn/34.12.2728. View

10.

Miller T, Wolin M, Conway de Macario E, Macario A . Isolation of Methanobrevibacter smithii from human feces. Appl Environ Microbiol. 1982; 43(1):227-32. PMC: 241804. DOI: 10.1128/aem.43.1.227-232.1982. View

11.

Miller T, Wolin M . Enumeration of Methanobrevibacter smithii in human feces. Arch Microbiol. 1982; 131(1):14-8. DOI: 10.1007/BF00451492. View

12.

Marthinsen D, Fleming S . Excretion of breath and flatus gases by humans consuming high-fiber diets. J Nutr. 1982; 112(6):1133-43. DOI: 10.1093/jn/112.6.1133. View

13.

KARLIN D, Jones R, Stroehlein J, Mastromarino A, Potter G . Breath methane excretion in patients with unresected colorectal cancer. J Natl Cancer Inst. 1982; 69(3):573-6. View

14.

Miller T, Wolin M . Stability of Methanobrevibacter smithii populations in the microbial flora excreted from the human large bowel. Appl Environ Microbiol. 1983; 45(1):317-8. PMC: 242276. DOI: 10.1128/aem.45.1.317-318.1983. View

15.

Bjorneklett A, JENSSEN E . Relationships between hydrogen (H2) and methane (CH4) production in man. Scand J Gastroenterol. 1982; 17(8):985-92. View

16.

Beaven J, Bjorneklett A, JENSSEN E, Blomhoff J, Skrede S . Pulmonary hydrogen and methane and plasma ammonia after the administration of lactulose or sorbitol. Scand J Gastroenterol. 1983; 18(3):343-7. DOI: 10.3109/00365528309181604. View

17.

Pique J, Pallares M, Cuso E, VILAR-BONET J, Gassull M . Methane production and colon cancer. Gastroenterology. 1984; 87(3):601-5. View

18.

Daniels L, Sparling R, Sprott G . The bioenergetics of methanogenesis. Biochim Biophys Acta. 1984; 768(2):113-63. DOI: 10.1016/0304-4173(84)90002-8. View

19.

Miller T, Weaver G, Wolin M . Methanogens and anaerobes in a colon segment isolated from the normal fecal stream. Appl Environ Microbiol. 1984; 48(2):449-50. PMC: 241538. DOI: 10.1128/aem.48.2.449-450.1984. View

20.

Metz G, Gassull M, Leeds A, Blendis L, Jenkins D . A simple method of measuring breath hydrogen in carbohydrate malabsorption by end-expiratory sampling. Clin Sci Mol Med. 1976; 50(3):237-40. DOI: 10.1042/cs0500237. View