Isolation and Characterization of a Mouse Y Chromosomal Repetitive Sequence

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1986 Jun 1

PMID 3721197

Citations 21

Authors

Y Nishioka

E Lamothe

Affiliations

Soon will be listed here.

Abstract

The Y chromosome plays a dominant role in mammalian sex determination, and characterization of this chromosome is essential to understand the mechanism responsible for testicular differentiation. Male mouse genomic DNA fragments, cloned into pBR322, were screened for the presence of Bkm (a female snake satellite DNA)-related sequences, and we obtained a clone (AC11) having a DNA fragment from the mouse Y chromosome. In addition to a Bkm-related sequence, this fragment contained a Y chromosomal repetitive sequence. DNA isolated from the XX sex-reversed male genome produced a hybridization pattern indistinguishable to that obtained with normal female DNA, suggesting that the AC11 sequence is not contained within the Y chromosomal DNA present in the sex-reversed male genome. Based on the hybridization patterns against mouse Y chromosomal DNA, AC11 classified 16 inbred laboratory strains into two categories; those with the Mus musculus musculus type Y chromosome and those with the M.m. domesticus type Y chromosome. Three European subspecies of Mus musculus (M.m. brevirostris, M.m. poschiavinus and M.m. praetextus) possessed the M.m. domesticus type Y chromosome, whereas the Japanese mouse, M.m. molossinus, had the M.m. musculus type Y chromosome. The survey was also extended to six other species that belong to the genus Mus, of which M. spretus and M. hortulamus showed significant amounts of AC11-related sequences in their Y chromosomes. The male-specific accumulation of AC11-related sequences was not found in M. caroli, M. cookii, M. pahari or M. platythrix. This marked difference among Mus species indicates that the amplification of AC11-related sequences in the mouse Y chromosome was a recent evolutionary event.

Citing Articles

Sequence and Structural Diversity of Mouse Y Chromosomes.

Morgan A, Pardo-Manuel de Villena F Mol Biol Evol. 2017; 34(12):3186-3204.

PMID: 29029271 PMC: 5850875. DOI: 10.1093/molbev/msx250.

Sequencing the mouse Y chromosome reveals convergent gene acquisition and amplification on both sex chromosomes.

Soh Y, Alfoldi J, Pyntikova T, Brown L, Graves T, Minx P Cell. 2014; 159(4):800-13.

PMID: 25417157 PMC: 4260969. DOI: 10.1016/j.cell.2014.09.052.

Rapid evolution of mouse Y centromere repeat DNA belies recent sequence stability.

Pertile M, Graham A, Choo K, Kalitsis P Genome Res. 2009; 19(12):2202-13.

PMID: 19737860 PMC: 2792177. DOI: 10.1101/gr.092080.109.

Live offspring from mice lacking the Y chromosome long arm gene complement.

Yamauchi Y, Riel J, Wong S, Ojarikre O, Burgoyne P, Ward M Biol Reprod. 2009; 81(2):353-61.

PMID: 19420387 PMC: 2849819. DOI: 10.1095/biolreprod.109.076307.

Identification of novel Y chromosome encoded transcripts by testis transcriptome analysis of mice with deletions of the Y chromosome long arm.

Toure A, Clemente E, Ellis P, Mahadevaiah S, Ojarikre O, Ball P Genome Biol. 2005; 6(12):R102.

PMID: 16356265 PMC: 1414076. DOI: 10.1186/gb-2005-6-12-r102.

References

White B, Tjio J . A mouse translocation with 38 and 39 chromosomes but normal N. F. Hereditas. 1967; 58(3):284-96. DOI: 10.1111/j.1601-5223.1967.tb02157.x. View

Bennett K, Hill R, Pietras D, Houston J, Heath J, Hastie N . Most highly repeated dispersed DNA families in the mouse genome. Mol Cell Biol. 1984; 4(8):1561-71. PMC: 368948. DOI: 10.1128/mcb.4.8.1561-1571.1984. View

Virgo N, Miller J . Hereditary vasopressin-resistant diabetes insipidus in SWV mice. Can J Physiol Pharmacol. 1974; 52(5):995-1011. DOI: 10.1139/y74-131. View

Rice M, OBrien S . Genetic variance of laboratory outbred Swiss mice. Nature. 1980; 283(5743):157-61. DOI: 10.1038/283157a0. View

Singh L, Purdom I, Jones K . Sex chromosome associated satellite DNA: evolution and conservation. Chromosoma. 1980; 79(2):137-57. DOI: 10.1007/BF01175181. View

Singh L, Jones K . Sex reversal in the mouse (Mus musculus) is caused by a recurrent nonreciprocal crossover involving the x and an aberrant y chromosome. Cell. 1982; 28(2):205-16. DOI: 10.1016/0092-8674(82)90338-5. View

Soriano P, Cuny G, Strauss F, Bernardi G . Sequence organization and genomic distribution of the major family of interspersed repeats of mouse DNA. Proc Natl Acad Sci U S A. 1982; 79(2):355-9. PMC: 345730. DOI: 10.1073/pnas.79.2.355. View

Eicher E, Washburn L, Whitney 3rd J, Morrow K . Mus poschiavinus Y chromosome in the C57BL/6J murine genome causes sex reversal. Science. 1982; 217(4559):535-7. DOI: 10.1126/science.7089579. View

Yonekawa H, Moriwaki K, Gotoh O, Miyashita N, Migita S, Bonhomme F . Origins of laboratory mice deduced from restriction patterns of mitochondrial DNA. Differentiation. 1982; 22(3):222-6. DOI: 10.1111/j.1432-0436.1982.tb01255.x. View

10.

Bishop C, Guellaen G, Geldwerth D, Voss R, Fellous M, Weissenbach J . Single-copy DNA sequences specific for the human Y chromosome. Nature. 1983; 303(5920):831-2. DOI: 10.1038/303831a0. View

11.

DOERFLER W . DNA methylation and gene activity. Annu Rev Biochem. 1983; 52:93-124. DOI: 10.1146/annurev.bi.52.070183.000521. View

12.

Guellaen G, Casanova M, Bishop C, Geldwerth D, Andre G, Fellous M . Human XX males with Y single-copy DNA fragments. Nature. 1984; 307(5947):172-3. DOI: 10.1038/307172a0. View

13.

Singh L, Phillips C, Jones K . The conserved nucleotide sequences of Bkm, which define Sxr in the mouse, are transcribed. Cell. 1984; 36(1):111-20. DOI: 10.1016/0092-8674(84)90079-5. View

14.

Eicher E, Washburn L . Inherited sex reversal in mice: identification of a new primary sex-determining gene. J Exp Zool. 1983; 228(2):297-304. DOI: 10.1002/jez.1402280213. View

15.

Lamar E, Palmer E . Y-encoded, species-specific DNA in mice: evidence that the Y chromosome exists in two polymorphic forms in inbred strains. Cell. 1984; 37(1):171-7. DOI: 10.1016/0092-8674(84)90312-x. View

16.

Gropp A, Winking H, ZECH L, Muller H . Robertsonian chromosomal variation and identification of metacentric chromosomes in feral mice. Chromosoma. 1972; 39(3):265-88. DOI: 10.1007/BF00290787. View