Preventive Effect and Mechanism of Tibetan Tea Extract on Thrombosis in Arachidonic Acid-induced Zebrafish Determined Via RNA-seq Transcriptome Profiles

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2023 May 19

PMID 37205684

Authors

Ning Wang

Chaohua Lan

Huiqiang Lu

Linman Li

Dalong Liao

Kewei Xu

Haiyan Sun

Yongqing Tang

Yumeng Wang

Jie Mei

Mengting Wei

Tao Wu

Hui Zhu

Affiliations

Soon will be listed here.

Abstract

Thrombosis is a key pathological event in cardiovascular diseases and is also the most important targeting process for their clinical management. In this study, arachidonic acid (AA) was used to induce thrombus formation in zebrafish larvae. Blood flow, red blood cell (RBCs) aggregation and cellular oxidative stress were measured to evaluate the antithrombotic effect of Tibetan tea (TT). Meanwhile, the potential molecular mechanism was further explored by transcriptome sequencing (RNA-seq). The results indicated that TT could significantly restore heart RBCs intensity of thrombotic zebrafish, whilst decreasing RBCs accumulation in the caudal vein. The transcriptome analysis revealed that the preventive effect of TT on thrombosis could be mostly attributed to changes in lipid metabolism related signaling pathways, such as fatty acid metabolism, glycerollipid metabolism, ECM-receptor interaction and steroid biosynthesis signaling pathway. This study demonstrated that Tibetan tea could alleviate thrombosis by reducing oxidative stress levels and regulating lipid metabolism.

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