Peptidomic Changes in Human Serous Colorectal Cancer Patients

Overview

Journal J Gastrointest Oncol

Date 2023 May 18

PMID 37201060

Authors

Jianghao Xu

Xingling Li

Wenlong Chen

Steven Yan Cheng

Jie Cheng

Cong Chen

Affiliations

Soon will be listed here.

Abstract

Background: Colorectal cancer (CRC) is the third most common cancer worldwide and one of the leading causes of cancer-related death. Peptidomics, considered a novel branch of proteomics, has an increasing range of applications in the screening, diagnosis, prognosis, and even monitoring of cancer. However, there is little information for peptidomics analysis in CRC.

Methods: In this study, a comparative peptidomic profiling was analyzed in 3 CRC tissue samples and 3 adjacent intestinal epithelial tissue samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Results: Among the total 133 nonredundant peptides identified, 59 were significantly differentially expressed in the CRC samples and benign colonic epithelium conditions [fold change (FC) >2, P<0.05]. Totals of 25 up-regulated and 34 down-regulated peptides were detected, respectively. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were applied to predict the possible functions of these relevant precursor proteins. To clearly identify the potential interaction network of peptide precursors, the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) was used to determine protein interactions and a possible central role in CRC.

Conclusions: Our results for the first time revealed the differentially expressed peptides between the serous CRC tissue and the adjacent intestinal epithelial tissue samples, and these prominently variable peptides might have an important potential role in occurrence and development of CRC.

Citing Articles

Clinical Peptidomics in Acute Leukemias: Current Advances and Future Perspectives.

Coutinho D, Freitas T, Silva Batista A, Quezado de Magalhaes M, Sabino A J Proteome Res. 2024; 23(12):5263-5273.

PMID: 39556650 PMC: 11629390. DOI: 10.1021/acs.jproteome.4c00807.

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