Biosynthesis of Silver Nanoparticles Using the Biofilm Supernatant of PA75 and Evaluation of Their Antibacterial, Antibiofilm, and Antitumor Activities

Overview

Journal Int J Nanomedicine

Publisher Dove Medical Press

Specialty Biotechnology

Date 2023 May 16

PMID 37192897

Authors

Fengjun Xia

Xiaoyan Tao

Haichen Wang

Jian Shui

Changhang Min

Yubing Xia

Jun Li

Mengli Tang

Zhaojun Liu

Yongmei Hu

Huidan Luo

Mingxiang Zou

Affiliations

Soon will be listed here.

Abstract

Purpose: As an under-explored biomaterial, bacterial biofilms have a wide range of applications in the green synthesis of nanomaterials. The biofilm supernatant of PA75 was used to synthesize novel silver nanoparticles (AgNPs). BF75-AgNPs were found to possess several biological properties.

Methods: In this study, we biosynthesized BF75-AgNPs using biofilm supernatant as the reducing agent, stabilizer, and dispersant and investigated their biopotential in terms of antibacterial, antibiofilm, and antitumor activities.

Results: The synthesized BF75-AgNPs demonstrated a typical face-centered cubic crystal structure; they were well dispersed; and they were spherical with a size of 13.899 ± 4.036 nm. The average zeta potential of the BF75-AgNPs was -31.0 ± 8.1 mV. The BF75-AgNPs exhibited strong antibacterial activities against the methicillin-resistant (MRSA), extended-spectrum beta-lactamase (ESBL-EC), extensively drug-resistant (XDR-KP), and carbapenem-resistant (CR-PA). Moreover, the BF75-AgNPs had a strong bactericidal effect on XDR-KP at 1/2 × MIC, and the expression level of reactive oxygen species (ROS) in bacteria was significantly increased. A synergistic effect was observed when the BF75-AgNPs and colistin were used for the co-treatment of two colistin-resistant XDR-KP strains, with fractional inhibitory concentration index (FICI) values of 0.281 and 0.187, respectively. Furthermore, the BF75-AgNPs demonstrated a strong biofilm inhibition activity and mature biofilm bactericidal activity against XDR-KP. The BF75-AgNPs also exhibited a strong antitumor activity against melanoma cells and low cytotoxicity against normal epidermal cells. In addition, the BF75-AgNPs increased the proportion of apoptotic cells in two melanoma cell lines, and the proportion of late apoptotic cells increased with BF75-AgNP concentration.

Conclusion: This study suggests that BF75-AgNPs synthesized from biofilm supernatant have broad prospects for antibacterial, antibiofilm, and antitumor applications.

Citing Articles

Advances in silver nanoparticles: a comprehensive review on their potential as antimicrobial agents and their mechanisms of action elucidated by proteomics.

Rodrigues A, Batista J, Rodrigues M, Thipe V, Minarini L, Lopes P Front Microbiol. 2024; 15:1440065.

PMID: 39149204 PMC: 11325591. DOI: 10.3389/fmicb.2024.1440065.

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