Sp. Nov., a Novel Actinobacterium Isolated from Soils of Weathering Dolomite

Strain KLBMP 9083, a novel actinobacterium, was isolated from weathered soils collected from a karst area in Anshun, Guizhou Province, PR China. The taxonomic position of strain KLBMP 9083 was studied using the polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain KLBMP 9083 formed a stabilized monophyletic clade with its closest relative strain CGMCC 1.13856 (98.4 % 16S rRNA gene sequence similarity). The peptidoglycan hydrolysates contained alanine, glutamic acid, threonine and lysine. The polar lipids were composed of diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified phosphoglycolipid, an unidentified phospholipid and an unidentified glycolipid. The predominant menaquinones were MK-9(H) (87.1 %), MK-9(H) (7.3 %) and MK-9(H) (5.6 %). The major fatty acids (>10 %) were anteiso-C and iso-C. The genomic DNA G+C content was 72.3 mol%. The digital DNA-DNA hybridization and average nucleotide identity values between strain KLBMP 9083 and CGMCC 1.13856 were 23.4 and 79.9 %, respectively. On the basis of morphological, chemotaxonomic and phylogenetic characteristics, strain KLBMP 9083 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KLBMP 9083 (=CGMCC 4.7737=NBRC 115577).