Urinary Biomarkers and Point-of-Care Urinalysis Devices for Early Diagnosis and Management of Disease: A Review

Overview

Journal Biomedicines

Specialty Biomedical Engineering

Date 2023 May 16

PMID 37189669

Authors

Beatriz Sequeira-Antunes

Hugo Alexandre Ferreira

Affiliations

Soon will be listed here.

Abstract

Biosensing and microfluidics technologies are transforming diagnostic medicine by accurately detecting biomolecules in biological samples. Urine is a promising biological fluid for diagnostics due to its noninvasive collection and wide range of diagnostic biomarkers. Point-of-care urinalysis, which integrates biosensing and microfluidics, has the potential to bring affordable and rapid diagnostics into the home to continuing monitoring, but challenges still remain. As such, this review aims to provide an overview of biomarkers that are or could be used to diagnose and monitor diseases, including cancer, cardiovascular diseases, kidney diseases, and neurodegenerative disorders, such as Alzheimer's disease. Additionally, the different materials and techniques for the fabrication of microfluidic structures along with the biosensing technologies often used to detect and quantify biological molecules and organisms are reviewed. Ultimately, this review discusses the current state of point-of-care urinalysis devices and highlights the potential of these technologies to improve patient outcomes. Traditional point-of-care urinalysis devices require the manual collection of urine, which may be unpleasant, cumbersome, or prone to errors. To overcome this issue, the toilet itself can be used as an alternative specimen collection and urinalysis device. This review then presents several smart toilet systems and incorporated sanitary devices for this purpose.

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References

Whelan P, Nelson A, Kim C, Tabib C, Preminger G, Turner N . Investigating Risk Factors for Urine Culture Contamination in Outpatient Clinics: A New Avenue for Diagnostic Stewardship. Antimicrob Steward Healthc Epidemiol. 2022; 2(1). PMC: 9016366. DOI: 10.1017/ash.2021.260. View

Liu B, Zhang L, Lv L, Wang Y, Liu D, Zhang X . Application of antibody array technology in the analysis of urinary cytokine profiles in patients with chronic kidney disease. Am J Nephrol. 2006; 26(5):483-90. DOI: 10.1159/000096871. View

Bouatra S, Aziat F, Mandal R, Guo A, Wilson M, Knox C . The human urine metabolome. PLoS One. 2013; 8(9):e73076. PMC: 3762851. DOI: 10.1371/journal.pone.0073076. View

Uchida K, Gotoh A . Measurement of cystatin-C and creatinine in urine. Clin Chim Acta. 2002; 323(1-2):121-8. DOI: 10.1016/s0009-8981(02)00177-8. View

Mushi M, Alex V, Seugendo M, Silago V, Mshana S . C - reactive protein and urinary tract infection due to Gram-negative bacteria in a pediatric population at a tertiary hospital, Mwanza, Tanzania. Afr Health Sci. 2020; 19(4):3217-3224. PMC: 7040338. DOI: 10.4314/ahs.v19i4.45. View

Rysz J, Gluba-Brzozka A, Franczyk B, Jablonowski Z, Cialkowska-Rysz A . Novel Biomarkers in the Diagnosis of Chronic Kidney Disease and the Prediction of Its Outcome. Int J Mol Sci. 2017; 18(8). PMC: 5578092. DOI: 10.3390/ijms18081702. View

Yao F, Hong X, Li S, Zhang Y, Zhao Q, Du W . Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods. J Alzheimers Dis. 2018; 65(2):421-431. DOI: 10.3233/JAD-180261. View

Zhu X, Ebrahimi B, Eirin A, Woollard J, Tang H, Jordan K . Renal Vein Levels of MicroRNA-26a Are Lower in the Poststenotic Kidney. J Am Soc Nephrol. 2014; 26(6):1378-88. PMC: 4446869. DOI: 10.1681/ASN.2014030248. View

Kim Y, Huh I, Kim J, Park S, Ryu S, Kim K . Integration of Traditional and Metabolomics Biomarkers Identifies Prognostic Metabolites for Predicting Responsiveness to Nutritional Intervention against Oxidative Stress and Inflammation. Nutrients. 2017; 9(3). PMC: 5372896. DOI: 10.3390/nu9030233. View

10.

Lopez-Giacoman S, Madero M . Biomarkers in chronic kidney disease, from kidney function to kidney damage. World J Nephrol. 2015; 4(1):57-73. PMC: 4317628. DOI: 10.5527/wjn.v4.i1.57. View

11.

Heger Z, Cernei N, Gumulec J, Masarik M, Eckschlager T, Hrabec R . Determination of common urine substances as an assay for improving prostate carcinoma diagnostics. Oncol Rep. 2014; 31(4):1846-54. DOI: 10.3892/or.2014.3054. View

12.

Schlebusch T, Fichtner W, Mertig M, Leonhardt S . Unobtrusive and comprehensive health screening using an intelligent toilet system. Biomed Tech (Berl). 2014; 60(1):17-29. DOI: 10.1515/bmt-2013-0140. View

13.

Sununta S, Rattanarat P, Chailapakul O, Praphairaksit N . Microfluidic Paper-based Analytical Devices for Determination of Creatinine in Urine Samples. Anal Sci. 2018; 34(1):109-113. DOI: 10.2116/analsci.34.109. View

14.

Alix-Panabieres C, Pantel K . Liquid Biopsy: From Discovery to Clinical Application. Cancer Discov. 2021; 11(4):858-873. DOI: 10.1158/2159-8290.CD-20-1311. View

15.

Luka G, Ahmadi A, Najjaran H, Alocilja E, DeRosa M, Wolthers K . Microfluidics Integrated Biosensors: A Leading Technology towards Lab-on-a-Chip and Sensing Applications. Sensors (Basel). 2015; 15(12):30011-31. PMC: 4721704. DOI: 10.3390/s151229783. View

16.

Jin X, Yun S, Jeong P, Kim I, Kim W, Park S . Diagnosis of bladder cancer and prediction of survival by urinary metabolomics. Oncotarget. 2014; 5(6):1635-45. PMC: 4039236. DOI: 10.18632/oncotarget.1744. View

17.

Cimmino I, Bravaccini S, Cerchione C . Urinary Biomarkers in Tumors: An Overview. Methods Mol Biol. 2021; 2292:3-15. DOI: 10.1007/978-1-0716-1354-2_1. View

18.

Feng S, Yang Y, Yang J, Gao Y, Cao J, Li Z . Urinary sediment CCL5 messenger RNA as a potential prognostic biomarker of diabetic nephropathy. Clin Kidney J. 2022; 15(3):534-544. PMC: 8862108. DOI: 10.1093/ckj/sfab186. View

19.

Selvaraju V, Ayine P, Fadamiro M, Babu J, Brown M, Geetha T . Urinary Biomarkers of Inflammation and Oxidative Stress Are Elevated in Obese Children and Correlate with a Marker of Endothelial Dysfunction. Oxid Med Cell Longev. 2019; 2019:9604740. PMC: 6817929. DOI: 10.1155/2019/9604740. View

20.

Miller I, Peters S, Overmyer K, Paulson B, Westphall M, Coon J . Real-time health monitoring through urine metabolomics. NPJ Digit Med. 2019; 2:109. PMC: 6848197. DOI: 10.1038/s41746-019-0185-y. View