Pavement Cells Distinguish Touch from Letting Go

Overview

Journal Nat Plants

Specialties Biology
Genetics

Date 2023 May 15

PMID 37188852

Authors

Alexander H Howell

Carsten Volkner

Patrick McGreevy

Kaare H Jensen

Rainer Waadt

Simon Gilroy

Hans-Henning Kunz

Winfried S Peters

Michael Knoblauch

Affiliations

Soon will be listed here.

Abstract

A micro-cantilever technique applied to individual leaf epidermis cells of intact Arabidopsis thaliana and Nicotiana tabacum synthesizing genetically encoded calcium indicators (R-GECO1 and GCaMP3) revealed that compressive forces induced local calcium peaks that preceded delayed, slowly moving calcium waves. Releasing the force evoked significantly faster calcium waves. Slow waves were also triggered by increased turgor and fast waves by turgor drops in pressure probe tests. The distinct characteristics of the wave types suggest different underlying mechanisms and an ability of plants to distinguish touch from letting go.

Citing Articles

Robust organ size in Arabidopsis is primarily governed by cell growth rather than cell division patterns.

Burda I, Brauns F, Clark F, Li C, Roeder A Development. 2024; 151(19).

PMID: 39324278 PMC: 11488635. DOI: 10.1242/dev.202531.

Straining the root on and off triggers local calcium signalling.

Audemar V, Guerringue Y, Frederick J, Vinet P, Melogno I, Babataheri A Proc Biol Sci. 2023; 290(2012):20231462.

PMID: 38052247 PMC: 10697804. DOI: 10.1098/rspb.2023.1462.

References

Chehab E, Eich E, Braam J . Thigmomorphogenesis: a complex plant response to mechano-stimulation. J Exp Bot. 2008; 60(1):43-56. DOI: 10.1093/jxb/ern315. View

Braam J, Davis R . Rain-, wind-, and touch-induced expression of calmodulin and calmodulin-related genes in Arabidopsis. Cell. 1990; 60(3):357-64. DOI: 10.1016/0092-8674(90)90587-5. View

Hamant O, Haswell E . Life behind the wall: sensing mechanical cues in plants. BMC Biol. 2017; 15(1):59. PMC: 5505048. DOI: 10.1186/s12915-017-0403-5. View

Landrein B, Ingram G . Connected through the force: mechanical signals in plant development. J Exp Bot. 2019; 70(14):3507-3519. DOI: 10.1093/jxb/erz103. View

Kurusu T, Nishikawa D, Yamazaki Y, Gotoh M, Nakano M, Hamada H . Plasma membrane protein OsMCA1 is involved in regulation of hypo-osmotic shock-induced Ca2+ influx and modulates generation of reactive oxygen species in cultured rice cells. BMC Plant Biol. 2012; 12:11. PMC: 3313898. DOI: 10.1186/1471-2229-12-11. View

Yuan F, Yang H, Xue Y, Kong D, Ye R, Li C . OSCA1 mediates osmotic-stress-evoked Ca2+ increases vital for osmosensing in Arabidopsis. Nature. 2014; 514(7522):367-71. DOI: 10.1038/nature13593. View

Basu D, Haswell E . The Mechanosensitive Ion Channel MSL10 Potentiates Responses to Cell Swelling in Arabidopsis Seedlings. Curr Biol. 2020; 30(14):2716-2728.e6. DOI: 10.1016/j.cub.2020.05.015. View

Finkler A, Ashery-Padan R, Fromm H . CAMTAs: calmodulin-binding transcription activators from plants to human. FEBS Lett. 2007; 581(21):3893-8. DOI: 10.1016/j.febslet.2007.07.051. View

Whalley H, Sargeant A, Steele J, Lacoere T, Lamb R, Saunders N . Transcriptomic analysis reveals calcium regulation of specific promoter motifs in Arabidopsis. Plant Cell. 2011; 23(11):4079-95. PMC: 3246331. DOI: 10.1105/tpc.111.090480. View

10.

Xu Y, Berkowitz O, Narsai R, De Clercq I, Hooi M, Bulone V . Mitochondrial function modulates touch signalling in Arabidopsis thaliana. Plant J. 2018; 97(4):623-645. DOI: 10.1111/tpj.14183. View

11.

Darwish E, Ghosh R, Ontiveros-Cisneros A, Tran H, Petersson M, De Milde L . Touch signaling and thigmomorphogenesis are regulated by complementary CAMTA3- and JA-dependent pathways. Sci Adv. 2022; 8(20):eabm2091. PMC: 9122320. DOI: 10.1126/sciadv.abm2091. View

12.

Matsumura M, Nomoto M, Itaya T, Aratani Y, Iwamoto M, Matsuura T . Mechanosensory trichome cells evoke a mechanical stimuli-induced immune response in Arabidopsis thaliana. Nat Commun. 2022; 13(1):1216. PMC: 8904797. DOI: 10.1038/s41467-022-28813-8. View

13.

Zhou L, Liu S, Wang P, Lu T, Xu F, Genin G . The Arabidopsis trichome is an active mechanosensory switch. Plant Cell Environ. 2016; 40(5):611-621. DOI: 10.1111/pce.12728. View

14.

Symonds V, Godoy A, Alconada T, Botto J, Juenger T, Casal J . Mapping quantitative trait loci in multiple populations of Arabidopsis thaliana identifies natural allelic variation for trichome density. Genetics. 2005; 169(3):1649-58. PMC: 1449524. DOI: 10.1534/genetics.104.031948. View

15.

Sato Y, Shimizu-Inatsugi R, Yamazaki M, Shimizu K, Nagano A . Plant trichomes and a single gene GLABRA1 contribute to insect community composition on field-grown Arabidopsis thaliana. BMC Plant Biol. 2019; 19(1):163. PMC: 6486987. DOI: 10.1186/s12870-019-1705-2. View

16.

Zhao Y, Araki S, Wu J, Teramoto T, Chang Y, Nakano M . An expanded palette of genetically encoded Ca²⁺ indicators. Science. 2011; 333(6051):1888-91. PMC: 3560286. DOI: 10.1126/science.1208592. View

17.

Hamilton E, Schlegel A, Haswell E . United in diversity: mechanosensitive ion channels in plants. Annu Rev Plant Biol. 2014; 66:113-37. PMC: 4470482. DOI: 10.1146/annurev-arplant-043014-114700. View

18.

Tatsumi H, Furuichi T, Nakano M, Toyota M, Hayakawa K, Sokabe M . Mechanosensitive channels are activated by stress in the actin stress fibres, and could be involved in gravity sensing in plants. Plant Biol (Stuttg). 2013; 16 Suppl 1:18-22. DOI: 10.1111/plb.12095. View

19.

Bellandi A, Papp D, Breakspear A, Joyce J, Johnston M, de Keijzer J . Diffusion and bulk flow of amino acids mediate calcium waves in plants. Sci Adv. 2022; 8(42):eabo6693. PMC: 9586480. DOI: 10.1126/sciadv.abo6693. View

20.

Johns S, Hagihara T, Toyota M, Gilroy S . The fast and the furious: rapid long-range signaling in plants. Plant Physiol. 2021; 185(3):694-706. PMC: 8133610. DOI: 10.1093/plphys/kiaa098. View