Reinforcement of Injectable Hydrogel for Meniscus Tissue Engineering by Using Cellulose Nanofiber from Cassava Pulp

Overview

Journal Polymers (Basel)

Publisher MDPI

Date 2023 May 13

PMID 37177235

Authors

Rachasit Jeencham

Tulyapruek Tawonsawatruk

Piya-On Numpaisal

Yupaporn Ruksakulpiwat

Affiliations

Soon will be listed here.

Abstract

Injectable hydrogels can be applied to treat damaged meniscus in minimally invasive conditions. Generally, injectable hydrogels can be prepared from various polymers such as polycaprolactone (PCL) and poly (N-isopropylacrylamide) (PNIPAAm). Poly (ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) block copolymer-diacrylate (PEO-PPO-PEO-DA) is an interesting polymer due to its biodegradability and can be prepared as water-insoluble injectable hydrogel after curing with UV light at low intensity. However, mechanical and cell adhesion properties are not optimal for these hydrogels. For the improved mechanical performance of the injectable hydrogel, cellulose nanofiber (CNF) extracted from cassava pulp was used as a reinforcing filler in this study. In addition, gelatin methacrylate (GelMA), the denatured form of collagen was used to enhance cell adhesion. PEO-PPO-PEO-DA/CNF/GelMA injectable hydrogels were prepared with 2-hydroxy-1-(4-(hydroxy ethoxy) phenyl)-2-methyl-1-propanone as a photoinitiator and then cured with UV light, 365 nm at 6 mW/cm. Physicochemical characteristics of the hydrogels and hydrogels with CNF were studied in detail including morphology characterization, pore size diameter, porosity, mechanical properties, water uptake, and swelling. In addition, cell viability was also studied. CNF-reinforced injectable hydrogels were successfully prepared after curing with UV light within 10 min with a thickness of 2 mm. CNF significantly improved the mechanical characteristics of injectable hydrogels. The incorporation of GelMA into the injectable hydrogels improved the viability of human cartilage stem/progenitor cells. At optimum formulation, 12%PEO-PPO-PEO-DA/0.5%CNF/3%GelMA injectable hydrogels significantly promoted cell viability (>80%) and also showed good physicochemical properties, which met tissue engineering requirements. In summary, this work shows that these novel injectable hydrogels have the potential for meniscus tissue engineering.

Citing Articles

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References

An Y, Kim J, Yim H, Han W, Park Y, Park H . Meniscus regeneration with injectable Pluronic/PMMA-reinforced fibrin hydrogels in a rabbit segmental meniscectomy model. J Tissue Eng. 2021; 12:20417314211050141. PMC: 8552387. DOI: 10.1177/20417314211050141. View

Celikkin N, Mastrogiacomo S, Jaroszewicz J, Walboomers X, Swieszkowski W . Gelatin methacrylate scaffold for bone tissue engineering: The influence of polymer concentration. J Biomed Mater Res A. 2017; 106(1):201-209. DOI: 10.1002/jbm.a.36226. View

Li R, Zhou C, Chen J, Luo H, Li R, Chen D . Synergistic osteogenic and angiogenic effects of KP and QK peptides incorporated with an injectable and self-healing hydrogel for efficient bone regeneration. Bioact Mater. 2022; 18:267-283. PMC: 8961307. DOI: 10.1016/j.bioactmat.2022.02.011. View

Voisin H, Bergstrom L, Liu P, Mathew A . Nanocellulose-Based Materials for Water Purification. Nanomaterials (Basel). 2017; 7(3). PMC: 5388159. DOI: 10.3390/nano7030057. View

Wang F, Li Y, Shen Y, Wang A, Wang S, Xie T . The functions and applications of RGD in tumor therapy and tissue engineering. Int J Mol Sci. 2013; 14(7):13447-62. PMC: 3742196. DOI: 10.3390/ijms140713447. View

Kim J, An Y, Yim H, Han W, Park Y, Park H . Injectable Fibrin/Polyethylene Oxide Semi-IPN Hydrogel for a Segmental Meniscal Defect Regeneration. Am J Sports Med. 2021; 49(6):1538-1550. DOI: 10.1177/0363546521998021. View

Cui S, Zhang S, Coseri S . An injectable and self-healing cellulose nanofiber-reinforced alginate hydrogel for bone repair. Carbohydr Polym. 2022; 300:120243. DOI: 10.1016/j.carbpol.2022.120243. View

Bi S, Wang P, Hu S, Li S, Pang J, Zhou Z . Construction of physical-crosslink chitosan/PVA double-network hydrogel with surface mineralization for bone repair. Carbohydr Polym. 2019; 224:115176. DOI: 10.1016/j.carbpol.2019.115176. View

Jiang Y, Wang H, Wang X, Yu X, Li H, Tang K . Preparation of gelatin-based hydrogels with tunable mechanical properties and modulation on cell-matrix interactions. J Biomater Appl. 2021; 36(5):902-911. DOI: 10.1177/08853282211018567. View

10.

Noori A, Ashrafi S, Vaez-Ghaemi R, Hatamian-Zaremi A, Webster T . A review of fibrin and fibrin composites for bone tissue engineering. Int J Nanomedicine. 2017; 12:4937-4961. PMC: 5516781. DOI: 10.2147/IJN.S124671. View

11.

Li Y, Wang Y, Shen C, Meng Q . Non-swellable F127-DA hydrogel with concave microwells for formation of uniform-sized vascular spheroids. RSC Adv. 2022; 10(72):44494-44502. PMC: 9058638. DOI: 10.1039/d0ra06188c. View

12.

Wu J, Ding Q, Dutta A, Wang Y, Huang Y, Weng H . An injectable extracellular matrix derived hydrogel for meniscus repair and regeneration. Acta Biomater. 2015; 16:49-59. DOI: 10.1016/j.actbio.2015.01.027. View

13.

Liu Q, Liu J, Qin S, Pei Y, Zheng X, Tang K . High mechanical strength gelatin composite hydrogels reinforced by cellulose nanofibrils with unique beads-on-a-string morphology. Int J Biol Macromol. 2020; 164:1776-1784. DOI: 10.1016/j.ijbiomac.2020.08.044. View

14.

Wang B, Liu J, Niu D, Wu N, Yun W, Wang W . Mussel-Inspired Bisphosphonated Injectable Nanocomposite Hydrogels with Adhesive, Self-Healing, and Osteogenic Properties for Bone Regeneration. ACS Appl Mater Interfaces. 2021; 13(28):32673-32689. DOI: 10.1021/acsami.1c06058. View

15.

Doench I, Ahn Tran T, David L, Montembault A, Viguier E, Gorzelanny C . Cellulose Nanofiber-Reinforced Chitosan Hydrogel Composites for Intervertebral Disc Tissue Repair. Biomimetics (Basel). 2019; 4(1). PMC: 6477598. DOI: 10.3390/biomimetics4010019. View

16.

Aarstad O, Heggset E, Pedersen I, Bjornoy S, Syverud K, Strand B . Mechanical Properties of Composite Hydrogels of Alginate and Cellulose Nanofibrils. Polymers (Basel). 2019; 9(8). PMC: 6418825. DOI: 10.3390/polym9080378. View

17.

Numpaisal P, Rothrauff B, Gottardi R, Chien C, Tuan R . Rapidly dissociated autologous meniscus tissue enhances meniscus healing: An in vitro study. Connect Tissue Res. 2016; 58(3-4):355-365. DOI: 10.1080/03008207.2016.1245727. View

18.

Meng L, Shao C, Cui C, Xu F, Lei J, Yang J . Autonomous Self-Healing Silk Fibroin Injectable Hydrogels Formed via Surfactant-Free Hydrophobic Association. ACS Appl Mater Interfaces. 2019; 12(1):1628-1639. DOI: 10.1021/acsami.9b19415. View