HPTLC Analysis and Chemical Composition of Selected Essential Oils

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2023 May 13

PMID 37175338

Authors

Aime Vazquez

Nurhayat Tabanca

Paul E Kendra

Affiliations

Soon will be listed here.

Abstract

Tea tree oil (TTO) is a volatile essential oil obtained by distillation, mainly from the Australian native plant (Maiden & Betche) Cheel (Myrtaceae). In this study, a comparative analysis of the chemical constituents of seven tea tree oils () and four other spp. oils (, (M), two chemotypes of , (M and M), and (M)) was carried out using gas chromatography-mass spectrometry (GC-MS) and high-performance thin-layer chromatography (HPTLC). Among the seven TTOs, terpinen-4-ol (37.66-44.28%), γ-terpinene (16.42-20.75%), α-terpinene (3.47-12.62%), α-terpineol (3.11-4.66%), and terpinolene (2.75-4.19%) were the most abundant compounds. On the other hand, the most abundant compounds of the other oils varied, such as 1,8-cineole (64.63%) in M oil, (E)-nerolidol (48.40%) and linalool (33.30%) in M oil, 1,8-cineole (52.20%) in M oil, and linalool (38.19%) and 1,8-cineole (27.57%) in M oil. HPTLC fingerprinting of oils enabled the discrimination of TTO oils from other spp. oils. Variation was observed in the profile of the R values among EOs. The present study shows that HPTLC is one of the best ways to identify and evaluate the quality control in authenticating TTOs, other EOs, or EOs from other species within the Myrtaceae.

Citing Articles

Development of a Combined 2D-MGD TLC/HPTLC Method for the Separation of Terpinen-4-ol and α-Terpineol from Tea Tree, , Essential Oil.

Vazquez A, Tabanca N Biomolecules. 2025; 15(1).

PMID: 39858541 PMC: 11764153. DOI: 10.3390/biom15010147.

Application of Microwave-Assisted Water Extraction (MAWE) to Fully Realize Various Physiological Activities of Leaf Extract.

Lin T, Leu J, Lai Y, Chang Y, Chung Y, Liu H Plants (Basel). 2024; 13(23).

PMID: 39683155 PMC: 11644774. DOI: 10.3390/plants13233362.

Antifungal Activity of Tea Tree ( Cheel) Essential Oils against the Main Onychomycosis-Causing Dermatophytes.

Mingorance Alvarez E, Villar Rodriguez J, Lopez Ripado O, Mayordomo R J Fungi (Basel). 2024; 10(10).

PMID: 39452627 PMC: 11508421. DOI: 10.3390/jof10100675.

References

Hammer K, Carson C, Riley T . In-vitro activity of essential oils, in particular Melaleuca alternifolia (tea tree) oil and tea tree oil products, against Candida spp. J Antimicrob Chemother. 1998; 42(5):591-5. DOI: 10.1093/jac/42.5.591. View

Sampson B, Tabanca N, Kirimer N, Demirci B, Baser K, Khan I . Insecticidal activity of 23 essential oils and their major compounds against adult Lipaphis pseudobrassicae (Davis) (Aphididae: Homoptera). Pest Manag Sci. 2005; 61(11):1122-8. DOI: 10.1002/ps.1100. View

Baglyas M, Ott P, Garadi Z, Glavnik V, Beni S, Vovk I . High-performance thin-layer chromatography - antibacterial assay first reveals bioactive clerodane diterpenes in giant goldenrod (Solidago gigantea Ait.). J Chromatogr A. 2022; 1677:463308. DOI: 10.1016/j.chroma.2022.463308. View

Tabanca N, Masi M, Epsky N, Nocera P, Cimmino A, Kendra P . Laboratory Evaluation of Natural and Synthetic Aromatic Compounds as Potential Attractants for Male Mediterranean fruit Fly, . Molecules. 2019; 24(13). PMC: 6651369. DOI: 10.3390/molecules24132409. View

Man A, Santacroce L, Jacob R, Mare A, Man L . Antimicrobial Activity of Six Essential Oils Against a Group of Human Pathogens: A Comparative Study. Pathogens. 2019; 8(1). PMC: 6471180. DOI: 10.3390/pathogens8010015. View

Yasin M, Younis A, Javed T, Akram A, Ahsan M, Shabbir R . River Tea Tree Oil: Composition, Antimicrobial and Antioxidant Activities, and Potential Applications in Agriculture. Plants (Basel). 2021; 10(10). PMC: 8540646. DOI: 10.3390/plants10102105. View

Silva J, Meixner M, McPheron B, Steck G, Sheppard W . Recent Mediterranean fruit fly (Diptera: Tephritidae) infestations in Florida--a genetic perspective. J Econ Entomol. 2004; 96(6):1711-8. DOI: 10.1093/jee/96.6.1711. View

Tabanca N, Nalbantsoy A, Kendra P, Demirci F, Demirci B . Chemical Characterization and Biological Activity of the Mastic Gum Essential Oils of Var. from Turkey. Molecules. 2020; 25(9). PMC: 7248992. DOI: 10.3390/molecules25092136. View

Kairey L, Agnew T, Bowles E, Barkla B, Wardle J, Lauche R . Efficacy and safety of (tea tree) oil for human health-A systematic review of randomized controlled trials. Front Pharmacol. 2023; 14:1116077. PMC: 10080088. DOI: 10.3389/fphar.2023.1116077. View

10.

Chouhan S, Sharma K, Guleria S . Antimicrobial Activity of Some Essential Oils-Present Status and Future Perspectives. Medicines (Basel). 2017; 4(3). PMC: 5622393. DOI: 10.3390/medicines4030058. View

11.

Tabanca N, Niogret J, Kendra P, Epsky N . TLC-Based Bioassay to Isolate Kairomones from Tea Tree Essential Oil That Attract Male Mediterranean Fruit Flies, (Wiedemann). Biomolecules. 2020; 10(5). PMC: 7277685. DOI: 10.3390/biom10050683. View

12.

Carson C, Hammer K, Riley T . Melaleuca alternifolia (Tea Tree) oil: a review of antimicrobial and other medicinal properties. Clin Microbiol Rev. 2006; 19(1):50-62. PMC: 1360273. DOI: 10.1128/CMR.19.1.50-62.2006. View

13.

Sakhanokho H, Sampson B, Tabanca N, Wedge D, Demirci B, Baser K . Chemical composition, antifungal and insecticidal activities of Hedychium essential oils. Molecules. 2013; 18(4):4308-27. PMC: 6270349. DOI: 10.3390/molecules18044308. View

14.

Kurtca M, Tumen I, Keskin H, Tabanca N, Yang X, Demirci B . Chemical Composition of Essential Oils from Leaves and Fruits of and Their Attractancy and Toxicity to Two Economically Important Tephritid Fruit Fly Species, and . Molecules. 2021; 26(24). PMC: 8704769. DOI: 10.3390/molecules26247504. View

15.

Luu-Dam N, Tabanca N, Estep A, Nguyen D, Kendra P . Insecticidal and Attractant Activities of Leaf Essential Oil against Two Major Pests from Diptera: (Culicidae) and (Tephritidae). Molecules. 2021; 26(8). PMC: 8072556. DOI: 10.3390/molecules26082311. View

16.

Lis-Balchin M, Hart S, Deans S . Pharmacological and antimicrobial studies on different tea-tree oils (Melaleuca alternifolia, Leptospermum scoparium or Manuka and Kunzea ericoides or Kanuka), originating in Australia and New Zealand. Phytother Res. 2000; 14(8):623-9. DOI: 10.1002/1099-1573(200012)14:8<623::aid-ptr763>3.0.co;2-z. View

17.

VANDENDOOL H, KRATZ P . A GENERALIZATION OF THE RETENTION INDEX SYSTEM INCLUDING LINEAR TEMPERATURE PROGRAMMED GAS-LIQUID PARTITION CHROMATOGRAPHY. J Chromatogr. 1963; 11:463-71. DOI: 10.1016/s0021-9673(01)80947-x. View

18.

Do T, Trettin I, De Vaumas R, Canigueral S, Valder C, Reich E . Proposal for a standardised method for the identification of essential oils by HPTLC. Pharmeur Bio Sci Notes. 2021; 2021:157-166. View

19.

Do T, Schmid M, Phanse M, Charegaonkar A, Sprecher H, Obkircher M . Development of the first universal mixture for use in system suitability tests for High-Performance Thin Layer Chromatography. J Chromatogr A. 2021; 1638:461830. DOI: 10.1016/j.chroma.2020.461830. View

20.

Ali A, Murphy C, Demirci B, Wedge D, Sampson B, Khan I . Insecticidal and biting deterrent activity of rose-scented geranium (Pelargonium spp.) essential oils and individual compounds against Stephanitis pyrioides and Aedes aegypti. Pest Manag Sci. 2013; 69(12):1385-92. DOI: 10.1002/ps.3518. View