Bull Semen Obtained on Beef Farms by Electroejaculation: Sperm Quality in the First Two Hours of Storing with Different Extenders and Holding Temperatures

Overview

Journal Animals (Basel)

Date 2023 May 13

PMID 37174597

Authors

Santiago Pernas

Aitor Fernandez-Novo

Clara Barrajon-Masa

Patricia Mozas

Natividad Perez-Villalobos

Barbara Martin-Maldonado

Agustin Oliet

Susana Astiz

Sonia S Perez-Garnelo

Affiliations

Soon will be listed here.

Abstract

Sperm quality decreases over time, so bull semen may need to be preserved after field collection. However, the effect of handling such semen samples from commercial farms and placing them in very short-term storage has not been elucidated. Therefore, ejaculate from 25 bulls from 1 dairy and 14 beef cattle farms were collected under farm conditions and evaluated for semen quality during the first two hours after collection. Two commercial extenders (AndroMed and BIOXcell) and two different storage temperatures (5 °C and room temperature) were used to evaluate the influence on semen quality and sperm kinetics in ejaculates grouped into three evaluation times, based on time since collection (Time 1: <75 min, = 7; Time 2: 75-105 min, = 11; and Time 3: 105-120 min, = 7). Classical semen parameters, sperm motion kinetics by CASA and colony-forming units were assessed. The differences between both extenders in curvilinear and straight-line velocities (VCL and VSL) for the different time groups (Time 2 and Time 3) were statistically significant for < 0.05. AndroMed showed lower VSL, straightness and linearity in sperm compared to BIOXcell ( < 0.05). In conclusion, AndroMed induced more curvilinear movement, while BIOXcell stimulated straighter motility.

References

Celeghini E, Arruda R, de Andrade A, Nascimento J, Raphael C, Rodrigues P . Effects that bovine sperm cryopreservation using two different extenders has on sperm membranes and chromatin. Anim Reprod Sci. 2007; 104(2-4):119-31. DOI: 10.1016/j.anireprosci.2007.02.001. View

Contri A, Valorz C, Faustini M, Wegher L, Carluccio A . Effect of semen preparation on casa motility results in cryopreserved bull spermatozoa. Theriogenology. 2010; 74(3):424-35. DOI: 10.1016/j.theriogenology.2010.02.025. View

Silva N, Leao K, Padua J, Marques T, Neto F, Dode M . Effect of different cryopreservation extenders added with antioxidants on semen quality and in vitro embryo production efficiency in cattle. An Acad Bras Cienc. 2021; 93(3):e20191229. DOI: 10.1590/0001-3765202120191229. View

Kumar N, Singh S, Jayachandran C . Pharmacokinetics of enrofloxacin and its active metabolite ciprofloxacin and its interaction with diclofenac after intravenous administration in buffalo calves. Vet J. 2003; 165(3):302-6. DOI: 10.1016/s1090-0233(02)00248-4. View

Dias E, Campanholi S, Rossi G, Freitas DellAqua C, Junior J, Papa F . Evaluation of cooling and freezing systems of bovine semen. Anim Reprod Sci. 2018; 195:102-111. DOI: 10.1016/j.anireprosci.2018.05.012. View

Kaka A, Wahid H, Rosnina Y, Yimer N, Khumran A, Sarsaifi K . α-Linolenic acid supplementation in BioXcell® extender can improve the quality of post-cooling and frozen-thawed bovine sperm. Anim Reprod Sci. 2014; 153:1-7. DOI: 10.1016/j.anireprosci.2014.12.001. View

Murphy E, Murphy C, OMeara C, Dunne G, Eivers B, Lonergan P . A comparison of semen diluents on the in vitro and in vivo fertility of liquid bull semen. J Dairy Sci. 2016; 100(2):1541-1554. DOI: 10.3168/jds.2016-11646. View

Chaveiro A, Machado L, Frijters A, Engel B, Woelders H . Improvement of parameters of freezing medium and freezing protocol for bull sperm using two osmotic supports. Theriogenology. 2005; 65(9):1875-90. DOI: 10.1016/j.theriogenology.2005.10.017. View

Aitken R, Sutton M, Warner P, Richardson D . Relationship between the movement characteristics of human spermatozoa and their ability to penetrate cervical mucus and zona-free hamster oocytes. J Reprod Fertil. 1985; 73(2):441-9. DOI: 10.1530/jrf.0.0730441. View

10.

van Wagtendonk-de Leeuw A, Haring R, den Daas J . Fertility results using bovine semen cryopreserved with extenders based on egg yolk and soy bean extract. Theriogenology. 2000; 54(1):57-67. DOI: 10.1016/S0093-691X(00)00324-1. View

11.

Thomas C, Garner D, DeJarnette J, Marshall C . Effect of cryopreservation of bovine sperm organelle function and viability as determined by flow cytometry. Biol Reprod. 1998; 58(3):786-93. DOI: 10.1095/biolreprod58.3.786. View

12.

Taaffe P, OMeara C, Stiavnicka M, Byrne C, Eivers B, Lonergan P . Increasing the frequency of ejaculate collection in young dairy bulls increases semen production and field fertility. Theriogenology. 2022; 182:45-52. DOI: 10.1016/j.theriogenology.2022.01.030. View

13.

Singh A, Kumar A, Honparkhe M, Kaur S, Kaur H, Ghuman S . Comparison of in vitro and in vivo fertilizing potential of buffalo bull semen frozen in egg yolk-, soya bean lecithin- and liposome-based extenders. Reprod Domest Anim. 2017; 53(1):195-202. DOI: 10.1111/rda.13092. View

14.

Zuidema D, Kerns K, Sutovsky P . An Exploration of Current and Perspective Semen Analysis and Sperm Selection for Livestock Artificial Insemination. Animals (Basel). 2021; 11(12). PMC: 8698075. DOI: 10.3390/ani11123563. View

15.

Nagy A, Polichronopoulos T, Gaspardy A, Solti L, Cseh S . Correlation between bull fertility and sperm cell velocity parameters generated by computer-assisted semen analysis. Acta Vet Hung. 2015; 63(3):370-81. DOI: 10.1556/004.2015.035. View

16.

Shojaei H, Kroetsch T, Wilde R, Blondin P, Kastelic J, Thundathil J . Moribund sperm in frozen-thawed semen, and sperm motion end points post-thaw and post-swim-up, are related to fertility in Holstein AI bulls. Theriogenology. 2011; 77(5):940-51. DOI: 10.1016/j.theriogenology.2011.09.026. View

17.

Satake N, Edwards S, Tutt D, McGowan M, Boe-Hansen G . Investigation of in vitro measurable sperm attributes and their influence on electroejaculated bull semen with a fixed-time artificial insemination protocol in Australian Bos indicus cattle. Reprod Domest Anim. 2017; 53(2):414-422. DOI: 10.1111/rda.13121. View

18.

Hyakutake T, Mori K, Sato K . Effects of surrounding fluid on motility of hyperactivated bovine sperm. J Biomech. 2018; 71:183-189. DOI: 10.1016/j.jbiomech.2018.02.009. View

19.

Hyakutake T, Sugita K, Ujifuku S, Sakurai R, Murakami R, Hayamizu Y . Experimental study on the effect of flow in microfluidic channel on bovine sperm navigation. J Biomech. 2021; 118:110290. DOI: 10.1016/j.jbiomech.2021.110290. View

20.

Singh K, Confer A, Step D, Rizzi T, Wyckoff 3rd J, Weng H . Cytokine expression by pulmonary leukocytes from calves challenged with wild-type and leukotoxin-deficient Mannheimia haemolytica. Vet J. 2011; 192(1):112-9. DOI: 10.1016/j.tvjl.2011.05.015. View