Comparative Transcriptomic and Evolutionary Analysis of FAD-like Genes of Brassica Species Revealed Their Role in Fatty Acid Biosynthesis and Stress Tolerance

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2023 May 12

PMID 37173631

Authors

Nabeel Shaheen

Uzair Muhammad Khan

Ayesha Farooq

Ummul Buneen Zafar

Sultan Habibullah Khan

Shakeel Ahmad

Muhammad Tehseen Azhar

Rana Muhammad Atif

Iqrar Ahmad Rana

Hyojin Seo

Affiliations

Soon will be listed here.

Abstract

Background: Fatty acid desaturases (FADs) are involved in regulating plant fatty acid composition by adding double bonds to growing hydrocarbon chain. Apart from regulating fatty acid composition FADs are of great importance, and are involved in stress responsiveness, plant development, and defense mechanisms. FADs have been extensively studied in crop plants, and are broadly classed into soluble and non-soluble fatty acids. However, FADs have not yet been characterized in Brassica carinata and its progenitors.

Results: Here we have performed comparative genome-wide identification of FADs and have identified 131 soluble and 28 non-soluble FADs in allotetraploid B. carinata and its diploid parents. Most soluble FAD proteins are predicted to be resided in endomembrane system, whereas FAB proteins were found to be localized in chloroplast. Phylogenetic analysis classed the soluble and non-soluble FAD proteins into seven and four clusters, respectively. Positive type of selection seemed to be dominant in both FADs suggesting the impact of evolution on these gene families. Upstream regions of both FADs were enriched in stress related cis-regulatory elements and among them ABRE type of elements were in abundance. Comparative transcriptomic data analysis output highlighted that FADs expression reduced gradually in mature seed and embryonic tissues. Moreover, under heat stress during seed and embryo development seven genes remained up-regulated regardless of external stress. Three FADs were only induced under elevated temperature whereas five genes were upregulated under Xanthomonas campestris stress suggesting their involvement in abiotic and biotic stress response.

Conclusions: The current study provides insights into the evolution of FADs and their role in B. carinata under stress conditions. Moreover, the functional characterization of stress-related genes would exploit their utilization in future breeding programs of B. carinata and its progenitors.

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