The Medicinal Activity of Lyophilized Aqueous Seed Extract of Lepidium Sativum L. in an Androgenic Alopecia Model

Overview

Journal Sci Rep

Specialty Science

Date 2023 May 11

PMID 37169776

Authors

Marzough Aziz Albalawi

Ahmed M Hafez

Seham S Elhawary

Nada K Sedky

Omnia F Hassan

Rofanda M Bakeer

Soha Abd El Hadi

Ahmed H El-Desoky

Sebaey Mahgoub

Fatma A Mokhtar

Affiliations

Soon will be listed here.

Abstract

This study evaluated the topical effect of Lepidium sativum lyophilized seed extract (LSLE) towards Sustanon-induced alopecia in male adult Wistar albino rats in vivo, compared to minoxidil topical reference standard drug (MRD). LC-MS/MS together with molecular networking was used to profile the metabolites of LSLE. LSLE treated group revealed significant changes in alopecia related biomarkers, perturbation of androgenic markers; decline in testosterone level and elevation in 5α-reductase (5-AR); decline in the cholesterol level. On the other hand, LSLE treated group showed improvement in vascular markers; CTGF, FGF and VEGF. Groups treated topically with minoxidil and LSLE showed significant improvement in hair length. LC-MS/MS profile of LSLE tentatively identified 17 constituents: mainly glucosinolates, flavonoid glycosides, alkaloids and phenolic acids. The results point to the potential role of LSLE in the treatment of alopecia through decreasing 5(alpha)-dihydrotestosterone levels. Molecular docking was attempted to evaluate the probable binding mode of identified compounds to androgen receptor (PDB code: 4K7A).

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