Prevalence and Characterization of Isolated from Clinical Bovine Mastitis Cases in Ningxia Hui Autonomous Region of China

Overview

Journal Infect Drug Resist

Publisher Dove Medical Press

Date 2023 May 11

PMID 37168514

Authors

Zeyi Liang

Jiahao Shen

Jing Liu

Xu Sun

Yayuan Yang

Yanan Lv

Juanshan Zheng

Xiaoqing Mou

Hongsheng Li

Xuezhi Ding

Feng Yang

Affiliations

Soon will be listed here.

Abstract

Purpose: This study aimed to investigate the prevalence and genetic characterization of isolates from clinical bovine mastitis in Ningxia Hui Autonomous Region of China.

Methods: was identified by the polymerase-chain reaction of 16S rRNA gene and sequencing. Antimicrobial susceptibility was tested by the disk diffusion method. Genes of resistance and virulence were determined by the PCR.

Results: Overall, were confirmed from 32 of 2897 (1.1%) mastitis milk samples. These isolates showed high resistance to cefazolin (30/32, 93.8%) and chloramphenicol (28/32, 87.5%). A 12.5% (4/32) of the isolates displayed multidrug resistance (MDR). The most prevalent resistant genes found in were (32/32, 100%) and (24/32, 75.0%; , 14/32, 43.8%; , 8/32, 25.0%; , 2/32, 6.3%) for extended-spectrum beta-lactamase, (28/32, 87.5%) and (16/32, 50.0%) for chloramphenicol resistance, (2/32, 6.3%) for carbapenemases, and (28/32, 87.5%), (26/32, 81.3%), (26/32, 81.3%) and (20/32, 62.5%) for efflux pumps. Moreover, all isolates carried virulence genes , and , and most of them contained (30/32, 93.8%), (26/32, 81.3%), (26/32, 81.3%), (26/32, 81.3%), (24/32, 75.0%) and (24/32, 75.0%).

Conclusion: To our knowledge, this is the first report of genetic determinants for antimicrobial resistance and virulence in isolated from bovine mastitis cases in China. These findings are useful for developing strategies for prevention and treatment of bovine mastitis caused by in China.

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