MiR-217 Regulates Autophagy Through OPG/RANKL/RANK in Giant Cell Tumors

Overview

Journal J Orthop Surg Res

Publisher Biomed Central

Specialty Orthopedics

Date 2023 May 10

PMID 37165403

Authors

Chenyang Meng

Boyong Jiang

Wanlin Liu

Lianjuan Wang

Zhenqun Zhao

Rui Bai

Yan Zhao

Affiliations

Soon will be listed here.

Abstract

Background: Increasing evidence suggests that microRNAs (miRNAs) play a crucial role in cancer development and progression. Our previous study showed remarkably lower levels of miR-217 in GCT cells and tissues, and miR-217 re-expression inhibited the occurrence and development of GCT in vitro; however, the associated mechanisms remain unknown. Thus, this study aimed to explore the mechanisms underlying the proliferation inhibitory effect of miR-217 in GCT cells.

Methods: The proliferative potential of the GCT cells was measured with an MTT assay and BrdU straining. Changes in GCT cell migration and invasion was assessed by a transwell assay. Finally, Western blot and RT-PCR assays were employed to evaluate OPG/RANKL/RANK signaling pathway-related protein expression.

Results: The excessive upregulation of miR-217 markedly suppressed GCT cell proliferation and tumorigenesis both in vitro and in vivo. miR-217 overexpression could inhibit the OPG/RANKL/RANK signaling pathway in vitro and in vivo. Furthermore, ALP activity was significantly decreased in GCT cells following miR-217 treatment. Importantly, miR-217 could inhibit autophagy-related protein expression and autophagosome/autolysosome formation in GCT cells and tissues.

Conclusion: These results suggest that miR-217 upregulation could inhibit the occurrence and development of GCT by blocking autophagy. These findings offer an effective therapeutic target to improve the survival rates of patients with CGT in the future.

Citing Articles

[miRNA Is Involved in the Pathogenesis of Multiple Diseases by Targeting Osteoprotegerin].

Zhou D, Yang D Sichuan Da Xue Xue Bao Yi Xue Ban. 2024; 55(3):777-782.

PMID: 38948285 PMC: 11211783. DOI: 10.12182/20240560607.

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