TorsinA is Essential for the Timing and Localization of Neuronal Nuclear Pore Complex Biogenesis

Overview

Journal bioRxiv

Date 2023 May 10

PMID 37162852

Authors

Sumin Kim

Sebastien Phan

Thomas R Shaw

Mark H Ellisman

Sarah L Veatch

Sami J Barmada

Samuel S Pappas

William T Dauer

Affiliations

Soon will be listed here.

Abstract

Nuclear pore complexes (NPCs) regulate information transfer between the nucleus and cytoplasm. NPC defects are linked to several neurological diseases, but the processes governing NPC biogenesis and spatial organization are poorly understood. Here, we identify a temporal window of strongly upregulated NPC biogenesis during neuronal maturation. We demonstrate that the AAA+ protein torsinA, whose loss of function causes the neurodevelopmental movement disorder DYT-TOR1A (DYT1) dystonia, coordinates NPC spatial organization during this period without impacting total NPC density. Using a new mouse line in which endogenous Nup107 is Halo-Tagged, we find that torsinA is essential for correct localization of NPC formation. In the absence of torsinA, the inner nuclear membrane buds excessively at sites of mislocalized, nascent NPCs, and NPC assembly completion is delayed. Our work implies that NPC spatial organization and number are independently regulated and suggests that torsinA is critical for the normal localization and assembly kinetics of NPCs.

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