Mouse Model of Latent Cryptococcal Infection and Reactivation

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2023 May 5

PMID 37145277

Authors

Ko Sato

Kazuyoshi Kawakami

Affiliations

Soon will be listed here.

Abstract

AbstractCryptococcus neoformans is an opportunistic fungal pathogen that frequently causes fatal meningoencephalitis in patients with impaired immune responses. This fungus, an intracellularly growing microbe, evades host immunity, leading to a latent infection (latent C. neoformans infection: LCNI), and cryptococcal disease is developed by its reactivation when host immunity is suppressed. Elucidation of the pathophysiology of LCNI is difficult due to the lack of mouse models. Here we show the established methods for LCNI and reactivation.

References

Lim T, Murphy J . Transfer of immunity to cryptococcosis by T-enriched splenic lymphocytes from Cryptococcus neoformans-sensitized mice. Infect Immun. 1980; 30(1):5-11. PMC: 551268. DOI: 10.1128/iai.30.1.5-11.1980. View

Koguchi Y, Kawakami K . Cryptococcal infection and Th1-Th2 cytokine balance. Int Rev Immunol. 2002; 21(4-5):423-38. DOI: 10.1080/08830180213274. View

Sato K, Kawakami K . Recognition of Cryptococcus neoformans by Pattern Recognition Receptors and its Role in Host Defense to This Infection. Med Mycol J. 2017; 58(3):J83-J90. DOI: 10.3314/mmj.17.011. View

Arora S, Olszewski M, Tsang T, McDonald R, Toews G, Huffnagle G . Effect of cytokine interplay on macrophage polarization during chronic pulmonary infection with Cryptococcus neoformans. Infect Immun. 2011; 79(5):1915-26. PMC: 3088136. DOI: 10.1128/IAI.01270-10. View

Tohyama M, Kawakami K, Futenma M, Saito A . Enhancing effect of oxygen radical scavengers on murine macrophage anticryptococcal activity through production of nitric oxide. Clin Exp Immunol. 1996; 103(3):436-41. PMC: 2200379. DOI: 10.1111/j.1365-2249.1996.tb08299.x. View

Hardison S, Ravi S, Wozniak K, Young M, Olszewski M, Wormley Jr F . Pulmonary infection with an interferon-gamma-producing Cryptococcus neoformans strain results in classical macrophage activation and protection. Am J Pathol. 2010; 176(2):774-85. PMC: 2808084. DOI: 10.2353/ajpath.2010.090634. View

Muller U, Stenzel W, Kohler G, Werner C, Polte T, Hansen G . IL-13 induces disease-promoting type 2 cytokines, alternatively activated macrophages and allergic inflammation during pulmonary infection of mice with Cryptococcus neoformans. J Immunol. 2007; 179(8):5367-77. DOI: 10.4049/jimmunol.179.8.5367. View

Zhang Y, Wang F, Tompkins K, McNamara A, Jain A, Moore B . Robust Th1 and Th17 immunity supports pulmonary clearance but cannot prevent systemic dissemination of highly virulent Cryptococcus neoformans H99. Am J Pathol. 2009; 175(6):2489-500. PMC: 2789623. DOI: 10.2353/ajpath.2009.090530. View

Murdock B, Huffnagle G, Olszewski M, Osterholzer J . Interleukin-17A enhances host defense against cryptococcal lung infection through effects mediated by leukocyte recruitment, activation, and gamma interferon production. Infect Immun. 2013; 82(3):937-48. PMC: 3957981. DOI: 10.1128/IAI.01477-13. View

10.

Szymczak W, Sellers R, Pirofski L . IL-23 dampens the allergic response to Cryptococcus neoformans through IL-17-independent and -dependent mechanisms. Am J Pathol. 2012; 180(4):1547-59. PMC: 3349902. DOI: 10.1016/j.ajpath.2011.12.038. View

11.

Hardison S, Wozniak K, Kolls J, Wormley Jr F . Interleukin-17 is not required for classical macrophage activation in a pulmonary mouse model of Cryptococcus neoformans infection. Infect Immun. 2010; 78(12):5341-51. PMC: 2981312. DOI: 10.1128/IAI.00845-10. View

12.

Wozniak K, Hole C, Yano J, Fidel P, Wormley F . Characterization of IL-22 and antimicrobial peptide production in mice protected against pulmonary Cryptococcus neoformans infection. Microbiology (Reading). 2014; 160(Pt 7):1440-1452. PMC: 4076872. DOI: 10.1099/mic.0.073445-0. View

13.

Sato K, Yamamoto H, Nomura T, Kasamatsu J, Miyasaka T, Tanno D . Production of IL-17A at Innate Immune Phase Leads to Decreased Th1 Immune Response and Attenuated Host Defense against Infection with . J Immunol. 2020; 205(3):686-698. DOI: 10.4049/jimmunol.1901238. View

14.

Feldmesser M, Tucker S, Casadevall A . Intracellular parasitism of macrophages by Cryptococcus neoformans. Trends Microbiol. 2001; 9(6):273-8. DOI: 10.1016/s0966-842x(01)02035-2. View

15.

Goldman D, Lee S, Mednick A, Montella L, Casadevall A . Persistent Cryptococcus neoformans pulmonary infection in the rat is associated with intracellular parasitism, decreased inducible nitric oxide synthase expression, and altered antibody responsiveness to cryptococcal polysaccharide. Infect Immun. 2000; 68(2):832-8. PMC: 97212. DOI: 10.1128/IAI.68.2.832-838.2000. View

16.

Tucker S, Casadevall A . Replication of Cryptococcus neoformans in macrophages is accompanied by phagosomal permeabilization and accumulation of vesicles containing polysaccharide in the cytoplasm. Proc Natl Acad Sci U S A. 2002; 99(5):3165-70. PMC: 122490. DOI: 10.1073/pnas.052702799. View

17.

Levitz S, Nong S, Seetoo K, Harrison T, Speizer R, Simons E . Cryptococcus neoformans resides in an acidic phagolysosome of human macrophages. Infect Immun. 1999; 67(2):885-90. PMC: 96400. DOI: 10.1128/IAI.67.2.885-890.1999. View

18.

Zaragoza O, Chrisman C, Castelli M, Frases S, Cuenca-Estrella M, Rodriguez-Tudela J . Capsule enlargement in Cryptococcus neoformans confers resistance to oxidative stress suggesting a mechanism for intracellular survival. Cell Microbiol. 2008; 10(10):2043-57. PMC: 4405381. DOI: 10.1111/j.1462-5822.2008.01186.x. View

19.

Vecchiarelli A, Pericolini E, Gabrielli E, Kenno S, Perito S, Cenci E . Elucidating the immunological function of the Cryptococcus neoformans capsule. Future Microbiol. 2013; 8(9):1107-16. DOI: 10.2217/fmb.13.84. View

20.

Alvarez M, Casadevall A . Phagosome extrusion and host-cell survival after Cryptococcus neoformans phagocytosis by macrophages. Curr Biol. 2006; 16(21):2161-5. DOI: 10.1016/j.cub.2006.09.061. View