Effect of Ascorbic Acid and Epidermal Growth Factor in a Rat Tibia Defect

Overview

Journal Acta Cir Bras

Specialty General Surgery

Date 2023 May 3

PMID 37132758

Authors

Victor Manuel Dominguez-Hernandez

Cecilia Hernandez-Flores

Alfonso Delgado

Rene Valdez-Mijares

Victor M Araujo-Monsalvo

Olivia Hernandez-Gonzalez

Affiliations

Soon will be listed here.

Abstract

Purpose: Bone repair aims to restore the anatomical, biomechanical, and functional integrity of the affected structure. Here we study the effects of ascorbic acid (AA) and epidermal growth factor (EGF) applied in a single dose and in combination on the repair of a noncritical bone defect model.

Methods: Twenty-four rats were divided into four groups: an intact G-1 control group, and three groups that underwent a noncritical bone defect in the right tibia: G-2 treated with AA, G-3 treated with EGF, and G-4 treated with AA in combination with EGF. After 21 days of treatment, rats were sacrificed, the tibias were dissected and a destructive biomechanical analysis of three-point flexion test was performed in a universal testing machine; the values of stiffness, resistance, maximum energy, and energy at maximum load were statistically compared.

Results: G-3 and G-4 recovered the biomechanical properties of strength and stiffness of an intact tibia 3 weeks after their application. Not so the energy and energy at maximum load. For G-2, only the stiffness of an intact tibia was recovered.

Conclusions: EGF and AA-EGF applied to a noncritical bone defect in the rat tibia favors the recovery of bone resistance and stiffness.

Citing Articles

Investigation into the effect of epidermal growth factor and hyaluronic acid on fracture healing in a rat femoral fracture model.

Kuzuca B, Doral M, Mangiavini L, Sapmaz E, Maffulli N, Koken M Acta Orthop Traumatol Turc. 2023; 57(5):229-236.

PMID: 37909682 PMC: 10724737. DOI: 10.5152/j.aott.2023.22163.

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