OL-FS13 Alleviates Cerebral Ischemia-reperfusion Injury by Inhibiting MiR-21-3p Expression

Overview

Journal Curr Neuropharmacol

Publisher Bentham Science Publishers

Date 2023 May 3

PMID 37132110

Authors

Naixin Liu

Yan Fan

Yilin Li

Yingxuan Zhang

Jiayi Li

Yinglei Wang

Zhuo Wang

Yixiang Liu

Yuansheng Li

Zijian Kang

Ying Peng

Zeqiong Ru

Meifeng Yang

Chengan Feng

Ying Wang

Xinwang Yang

Affiliations

Soon will be listed here.

Abstract

Background: OL-FS13, a neuroprotective peptide derived from Odorrana livida, can alleviate cerebral ischemia-reperfusion (CI/R) injury, although the specific underlying mechanism remains to be further explored.

Objective: The effect of miR-21-3p on the neural-protective effects of OL-FS13 was examined.

Methods: In this study, the multiple genome sequencing analysis, double luciferase experiment, RT-qPCR, and Western blotting were used to explore the mechanism of OL-FS13.

Results: Showed that over-expression of miR-21-3p against the protective effects of OL-FS13 on oxygen- glucose deprivation/re-oxygenation (OGD/R)-damaged pheochromocytoma (PC12) cells and in CI/R-injured rats. miR-21-3p was then found to target calcium/calmodulin-dependent protein kinase 2 (CAMKK2), and its overexpression inhibited the expression of CAMKK2 and phosphorylation of its downstream adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK), thereby inhibiting the therapeutic effects of OL-FS13 on OGD/R and CI/R. Inhibition of CAMKK2 also antagonized up-regulated of nuclear factor erythroid 2-related factor 2 (Nrf-2) by OL-FS13, thereby abolishing the antioxidant activity of the peptide.

Conclusion: Our results showed that OL-FS13 alleviated OGD/R and CI/R by inhibiting miR-21-3p to activate the CAMKK2/AMPK/Nrf-2 axis.

Citing Articles

The Role of microRNAs in Epigenetic Regulation of Signaling Pathways in Neurological Pathologies.

Tregub P, Ibrahimli I, Averchuk A, Salmina A, Litvitskiy P, Manasova Z Int J Mol Sci. 2023; 24(16).

PMID: 37629078 PMC: 10454825. DOI: 10.3390/ijms241612899.

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