Protective Role of Vitamin D3 in a Rat Model of Hyperthyroid-induced Cardiomyopathy

Overview

Journal J Tradit Complement Med

Specialty Rehabilitation Medicine

Date 2023 May 2

PMID 37128195

Authors

Heba Rady Salem

Ghada Adel Hegazy

Rania Abdallah

Reda Aa Abo-Elsoud

Affiliations

Soon will be listed here.

Abstract

Background And Aim: Several studies have reported the cardioprotective effect of vitamin D. Thus, this study aimed to investigate the possible cardioprotective effect of vitamin D3 in hyperthyroid-induced cardiomyopathy rat model.

Experimental Procedure: Rats were divided into 3 groups: control group; hyperthyroid group, rats were administrated l-thyroxine sodium daily for 4 weeks; and hyperthyroid + vitamin D3 treated group, rats were treated with l-thyroxine sodium for 4 weeks daily, and received the vitamin D3 for the same duration. After 4 weeks, electrocardiogram (ECG) was recorded. Then, blood samples were collected for biochemical analysis. After that, the final body weight was measured, and the rats were sacrificed. Finally, the hearts were excised, weighed and were prepared for histological examination by hematoxylin and eosin, and immunohistochemistry assessment of caspase-3 and proliferating cell nuclear antigen (PCNA).

Results: Hyperthyroid rats showed significant ECG changes, increased serum levels of cardiac biomarkers, fibroblast growth factor-23 (FGF23), malondialdehyde, antioxidant enzymes, tumor necrosis factor-alpha (TNF-α) and relative heart weight compared with the control rats. Vitamin D3 coadministration with l-thyroxine resulted in significant improvement in thyroid profile, ECG parameters, significant decrease of cardiac biomarkers, FGF23, malondialdehyde, TNF-α and relative heart weight, and significant decrease of the antioxidant enzymes compared with the hyperthyroid rats. The histological study was consistent with the biochemical results. Hyperthyroid rats showed upregulation of caspase-3 and PCNA in the myocardium compared with control group. Vitamin D3 treated rats showed downregulation of caspase-3 and PCNA.

Conclusion: Vitamin D3 provides cardioprotective effects in hyperthyroid rats.

Citing Articles

Cardioprotective effect of Vitamin D on cardiac hypertrophy through improvement of mitophagy and apoptosis in an experimental rat model of levothyroxine -induced hyperthyroidism.

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PMID: 39249564 DOI: 10.1007/s11033-024-09897-5.

SGLT2 inhibitor as a potential therapeutic approach in hyperthyroidism-induced cardiopulmonary injury in rats.

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References

Han S, Bai E, Jin G, He C, Guo X, Wang L . Expression and clinical significance of YAP, TAZ, and AREG in hepatocellular carcinoma. J Immunol Res. 2014; 2014:261365. PMC: 4016924. DOI: 10.1155/2014/261365. View

Bodey B, Bodey V, Siegel S, Nasir A, Coppola D, Hakam A . Immunocytochemical detection of members of the caspase cascade of apoptosis in high-grade astrocytomas. In Vivo. 2004; 18(5):593-602. View

Oliveros-Ruiz L, Vallejo M, Diez Canseco L, Cardenas M, Hermosillo J . Determinants of thyrotoxic cardiomyopathy recovery. Biomed Res Int. 2013; 2013:452709. PMC: 3780617. DOI: 10.1155/2013/452709. View

Park S, Cho M, Kim S, Rhee Y, Kang E, Ahn C . The adaptation and relationship of FGF-23 to changes in mineral metabolism in Graves' disease. Clin Endocrinol (Oxf). 2007; 66(6):854-8. DOI: 10.1111/j.1365-2265.2007.02824.x. View

Klein I, Ojamaa K . Thyroid hormone and the cardiovascular system. N Engl J Med. 2001; 344(7):501-9. DOI: 10.1056/NEJM200102153440707. View

Razvi S, Jabbar A, Pingitore A, Danzi S, Biondi B, Klein I . Thyroid Hormones and Cardiovascular Function and Diseases. J Am Coll Cardiol. 2018; 71(16):1781-1796. DOI: 10.1016/j.jacc.2018.02.045. View

Guler G, Dasdelen D, Baltaci S, Sivrikaya A, Baltaci A, Mogulkoc R . The effects of thyroid dysfunction on DNA damage and apoptosis in liver and heart tissues of rats. Horm Mol Biol Clin Investig. 2021; 43(1):47-53. DOI: 10.1515/hmbci-2021-0059. View

Gardner D, Chen S, Glenn D . Vitamin D and the heart. Am J Physiol Regul Integr Comp Physiol. 2013; 305(9):R969-77. PMC: 3840320. DOI: 10.1152/ajpregu.00322.2013. View

Costilla M, Macri Delbono R, Klecha A, Cremaschi G, Arcos M . Oxidative Stress Produced by Hyperthyroidism Status Induces the Antioxidant Enzyme Transcription through the Activation of the Nrf-2 Factor in Lymphoid Tissues of Balb/c Mice. Oxid Med Cell Longev. 2019; 2019:7471890. PMC: 6589208. DOI: 10.1155/2019/7471890. View

10.

Shimada T, Hasegawa H, Yamazaki Y, Muto T, Hino R, Takeuchi Y . FGF-23 is a potent regulator of vitamin D metabolism and phosphate homeostasis. J Bone Miner Res. 2004; 19(3):429-35. DOI: 10.1359/JBMR.0301264. View

11.

Bae S, Singh S, Yu H, Lee J, Cho B, Kang P . Vitamin D signaling pathway plays an important role in the development of heart failure after myocardial infarction. J Appl Physiol (1985). 2013; 114(8):979-87. PMC: 3633431. DOI: 10.1152/japplphysiol.01506.2012. View

12.

Chainy G, Sahoo D . Hormones and oxidative stress: an overview. Free Radic Res. 2019; 54(1):1-26. DOI: 10.1080/10715762.2019.1702656. View

13.

Siu C, Yeung C, Lau C, Kung A, Tse H . Incidence, clinical characteristics and outcome of congestive heart failure as the initial presentation in patients with primary hyperthyroidism. Heart. 2006; 93(4):483-7. PMC: 1861478. DOI: 10.1136/hrt.2006.100628. View

14.

Erben R . Physiological Actions of Fibroblast Growth Factor-23. Front Endocrinol (Lausanne). 2018; 9:267. PMC: 5985418. DOI: 10.3389/fendo.2018.00267. View

15.

Grais I, Sowers J . Thyroid and the heart. Am J Med. 2014; 127(8):691-8. PMC: 4318631. DOI: 10.1016/j.amjmed.2014.03.009. View

16.

Lu E, Ratnayake J, Rich A . Assessment of proliferating cell nuclear antigen (PCNA) expression at the invading front of oral squamous cell carcinoma. BMC Oral Health. 2019; 19(1):233. PMC: 6822390. DOI: 10.1186/s12903-019-0928-9. View

17.

Jabbar A, Pingitore A, Pearce S, Zaman A, Iervasi G, Razvi S . Thyroid hormones and cardiovascular disease. Nat Rev Cardiol. 2016; 14(1):39-55. DOI: 10.1038/nrcardio.2016.174. View

18.

Martineau A, Thummel K, Wang Z, Jolliffe D, Boucher B, Griffin S . Differential Effects of Oral Boluses of Vitamin D2 vs Vitamin D3 on Vitamin D Metabolism: A Randomized Controlled Trial. J Clin Endocrinol Metab. 2019; 104(12):5831-5839. PMC: 6797055. DOI: 10.1210/jc.2019-00207. View

19.

Osuna P, Udovcic M, Sharma M . Hyperthyroidism and the Heart. Methodist Debakey Cardiovasc J. 2017; 13(2):60-63. PMC: 5512680. DOI: 10.14797/mdcj-13-2-60. View

20.

Bielecka-Dabrowa A, Mikhailidis D, Rysz J, Banach M . The mechanisms of atrial fibrillation in hyperthyroidism. Thyroid Res. 2009; 2(1):4. PMC: 2680813. DOI: 10.1186/1756-6614-2-4. View